THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM
Aim of the study: Honey has been historically recognized as a traditional therapeutic agent for microbial infections due to its biologically active compounds, which confer antibacterial, anti-inflammatory, and antioxidant properties. This study aims to chemically characterize and evaluate the antimi...
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| Format: | Article |
| Language: | English |
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Romanian Society of Oral Rehabilitation
2025-06-01
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| Series: | Romanian Journal of Oral Rehabilitation |
| Online Access: | https://rjor.ro/the-effect-of-linden-honey-of-oral-microbiom-and-biofilm/ |
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| author | Alexandru Nan Marioara Nicoleta Caraba Daniela Puscasiu Cristian Sebastian Vlad Laria-Maria Trusculescu Ion Valeriu Caraba Matin Asad Salehi Gabi Dumitrescu |
| author_facet | Alexandru Nan Marioara Nicoleta Caraba Daniela Puscasiu Cristian Sebastian Vlad Laria-Maria Trusculescu Ion Valeriu Caraba Matin Asad Salehi Gabi Dumitrescu |
| author_sort | Alexandru Nan |
| collection | DOAJ |
| description | Aim of the study: Honey has been historically recognized as a traditional therapeutic agent for microbial infections due to its biologically active compounds, which confer antibacterial, anti-inflammatory, and antioxidant properties. This study aims to chemically characterize and evaluate the antimicrobial potential of linden honey sourced from an ecological apiary in western Romania. Materials and methods: Physico-chemical analyses were conducted to determine key parameters, including impurity levels, water content, acidity, pH, and reducing sugar concentration, all of which were found to be within the established regulatory standards. The total phenolic content was assessed to confirm the antioxidant properties of the honey. Microbiological evaluations were performed to determine inhibition rates and biofilm formation inhibition capacity against both Gram-positive and Gram-negative bacterial strains, including standard strains and those isolated from patients. The anti-biofilm potential was assessed using 2,3,5-triphenyl-tetrazolium chloride (TTC), while anti-biofilm activity was quantified via the crystal violet assay. Results: The antimicrobial and anti-biofilm effects of linden honey were observed across all tested bacterial strains, with variations depending on the honey concentration and bacterial type. The honey exhibited both bacteriostatic and bacteriolytic effects, influenced by concentration and bacterial susceptibility. Notably, the antibacterial efficacy differed when tested on standardized bacterial strains, patient-isolated strains, and antibiotic-resistant strains. Conclusions: The chemical composition of linden honey played a significant role in determining its antimicrobial and anti-biofilm efficacy. The findings support the potential of linden honey as a natural antimicrobial agent, with its effects being concentration-dependent and influenced by bacterial strain characteristics. These results contribute to the growing body of research on the therapeutic applications of honey in combating bacterial infections and biofilm formation.
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| format | Article |
| id | doaj-art-195348d1e7fa4d9d991b2f7a16d5e250 |
| institution | Kabale University |
| issn | 2066-7000 2601-4661 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Romanian Society of Oral Rehabilitation |
| record_format | Article |
| series | Romanian Journal of Oral Rehabilitation |
| spelling | doaj-art-195348d1e7fa4d9d991b2f7a16d5e2502025-08-20T04:01:57ZengRomanian Society of Oral RehabilitationRomanian Journal of Oral Rehabilitation2066-70002601-46612025-06-01172415110.62610/RJOR.2025.2.17.3THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILMAlexandru Nan0Marioara Nicoleta Caraba1Daniela Puscasiu2Cristian Sebastian Vlad3Laria-Maria Trusculescu4Ion Valeriu Caraba5Matin Asad Salehi6Gabi Dumitrescu7Doctoral School “Engineering of Vegetable and Animal Resources”, University of Life Sciences “King Mihai I” from TimişoaraCellular and Molecular Biology Department, “Victor Babes” University of Medicine and Pharmacy of TimisoaraCellular and Molecular Biology Department, “Victor Babes” University of Medicine and Pharmacy of Timisoara"Biochemistry and Pharmacology Department, “Victor Babes” University of Medicine and Pharmacy of Timisoara""Department of Management and Communication in Dental Medicine, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy of Timisoara""Faculty of Bioengineering of Animal Resources, University of Life Sciences “King Mihai I” from Timisoara"Doctoral School of Dental Medicine, University of Medicine and Pharmacy, 9 Revolutiei 1989 Bv., 300041, Timisoara, RomaniaFaculty of Bioengineering of Animal Resources, University of Life Sciences “King Mihai I” from TimisoaraAim of the study: Honey has been historically recognized as a traditional therapeutic agent for microbial infections due to its biologically active compounds, which confer antibacterial, anti-inflammatory, and antioxidant properties. This study aims to chemically characterize and evaluate the antimicrobial potential of linden honey sourced from an ecological apiary in western Romania. Materials and methods: Physico-chemical analyses were conducted to determine key parameters, including impurity levels, water content, acidity, pH, and reducing sugar concentration, all of which were found to be within the established regulatory standards. The total phenolic content was assessed to confirm the antioxidant properties of the honey. Microbiological evaluations were performed to determine inhibition rates and biofilm formation inhibition capacity against both Gram-positive and Gram-negative bacterial strains, including standard strains and those isolated from patients. The anti-biofilm potential was assessed using 2,3,5-triphenyl-tetrazolium chloride (TTC), while anti-biofilm activity was quantified via the crystal violet assay. Results: The antimicrobial and anti-biofilm effects of linden honey were observed across all tested bacterial strains, with variations depending on the honey concentration and bacterial type. The honey exhibited both bacteriostatic and bacteriolytic effects, influenced by concentration and bacterial susceptibility. Notably, the antibacterial efficacy differed when tested on standardized bacterial strains, patient-isolated strains, and antibiotic-resistant strains. Conclusions: The chemical composition of linden honey played a significant role in determining its antimicrobial and anti-biofilm efficacy. The findings support the potential of linden honey as a natural antimicrobial agent, with its effects being concentration-dependent and influenced by bacterial strain characteristics. These results contribute to the growing body of research on the therapeutic applications of honey in combating bacterial infections and biofilm formation. https://rjor.ro/the-effect-of-linden-honey-of-oral-microbiom-and-biofilm/ |
| spellingShingle | Alexandru Nan Marioara Nicoleta Caraba Daniela Puscasiu Cristian Sebastian Vlad Laria-Maria Trusculescu Ion Valeriu Caraba Matin Asad Salehi Gabi Dumitrescu THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM Romanian Journal of Oral Rehabilitation |
| title | THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM |
| title_full | THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM |
| title_fullStr | THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM |
| title_full_unstemmed | THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM |
| title_short | THE EFFECT OF LINDEN HONEY OF ORAL MICROBIOM AND BIOFILM |
| title_sort | effect of linden honey of oral microbiom and biofilm |
| url | https://rjor.ro/the-effect-of-linden-honey-of-oral-microbiom-and-biofilm/ |
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