Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials
Cello-oligosaccharides (COS) are products of the preliminary hydrolysis of cellulose. They have been the subject of significant research and application potential across various fields, including food, feed, and biotechnology. This study explored an eco-friendly, efficient process for producing COS...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2025-06-01
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| Series: | BioResources |
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| Online Access: | https://ojs.bioresources.com/index.php/BRJ/article/view/24409 |
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| author | Hong Chen Wenbo Chen Fanqin Yang Gang Xu Yin Cao Shanglian Hu Bo Zhao |
| author_facet | Hong Chen Wenbo Chen Fanqin Yang Gang Xu Yin Cao Shanglian Hu Bo Zhao |
| author_sort | Hong Chen |
| collection | DOAJ |
| description | Cello-oligosaccharides (COS) are products of the preliminary hydrolysis of cellulose. They have been the subject of significant research and application potential across various fields, including food, feed, and biotechnology. This study explored an eco-friendly, efficient process for producing COS from bamboo biomass. Subsequently, the optimal hydrolysis conditions using microcrystalline cellulose as the substrate were determined to establish the best process for converting bamboo cellulose into COS. The resulting hydrolyzate was analyzed, with cellobiose content (mg/L) serving as the response variable to identify the optimal conditions for pH, hydrolysis temperature, enzyme addition amount, substrate addition amount, reaction time, and inhibitor addition amount. Finally, various bamboo pretreatment technologies and cellulose hydrolysis methods were integrated to determine the most suitable hydrolysis technology for bamboo cellulose. The results of this study demonstrate that enzymatic hydrolysis can be employed as a production method to convert bamboo cellulose to COS. |
| format | Article |
| id | doaj-art-194a5f035ddc44fb8fa4bd9efcbf6c34 |
| institution | Kabale University |
| issn | 1930-2126 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj-art-194a5f035ddc44fb8fa4bd9efcbf6c342025-08-20T03:24:39ZengNorth Carolina State UniversityBioResources1930-21262025-06-01203593259513190Enzymatic Preparation of Cello-oligosaccharides Using Bamboo MaterialsHong Chen0Wenbo Chen1Fanqin Yang2Gang Xu3Yin Cao4Shanglian Hu5Bo Zhao6School of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Bamboo Research Institute, Southwest University of Science and Technology, Mianyang 621010, ChinaCello-oligosaccharides (COS) are products of the preliminary hydrolysis of cellulose. They have been the subject of significant research and application potential across various fields, including food, feed, and biotechnology. This study explored an eco-friendly, efficient process for producing COS from bamboo biomass. Subsequently, the optimal hydrolysis conditions using microcrystalline cellulose as the substrate were determined to establish the best process for converting bamboo cellulose into COS. The resulting hydrolyzate was analyzed, with cellobiose content (mg/L) serving as the response variable to identify the optimal conditions for pH, hydrolysis temperature, enzyme addition amount, substrate addition amount, reaction time, and inhibitor addition amount. Finally, various bamboo pretreatment technologies and cellulose hydrolysis methods were integrated to determine the most suitable hydrolysis technology for bamboo cellulose. The results of this study demonstrate that enzymatic hydrolysis can be employed as a production method to convert bamboo cellulose to COS.https://ojs.bioresources.com/index.php/BRJ/article/view/24409bamboocello-oligosaccharidesenzymolysispretreatmenttechnology optimization |
| spellingShingle | Hong Chen Wenbo Chen Fanqin Yang Gang Xu Yin Cao Shanglian Hu Bo Zhao Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials BioResources bamboo cello-oligosaccharides enzymolysis pretreatment technology optimization |
| title | Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials |
| title_full | Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials |
| title_fullStr | Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials |
| title_full_unstemmed | Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials |
| title_short | Enzymatic Preparation of Cello-oligosaccharides Using Bamboo Materials |
| title_sort | enzymatic preparation of cello oligosaccharides using bamboo materials |
| topic | bamboo cello-oligosaccharides enzymolysis pretreatment technology optimization |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/24409 |
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