Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats
Exertional heat stroke (EHS) is a life-threatening condition characterized by hyperthermia and multi-organ dysfunction, often associated with intestinal barrier disruption. This study evaluated the protective effects of Huoxiang Zhengqi Dropping Pills (HXZQD) against EHS in a rat model. HXZQD was ad...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1534713/full |
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| author | Lei Li Lei Li Lei Li Jun Ma Jun Ma Zeshi Li Zeshi Li Juelin Chen Jiawei Zhou Yawei Wang Yankun Pei Yitong Gong Jianyao You Yangyang Cao Man Wang Jikuai Chen Wenjun Chang Weiyi Ma Hanyu Zhu Chuhan Xiang Shuogui Xu Qing Song Qing Song |
| author_facet | Lei Li Lei Li Lei Li Jun Ma Jun Ma Zeshi Li Zeshi Li Juelin Chen Jiawei Zhou Yawei Wang Yankun Pei Yitong Gong Jianyao You Yangyang Cao Man Wang Jikuai Chen Wenjun Chang Weiyi Ma Hanyu Zhu Chuhan Xiang Shuogui Xu Qing Song Qing Song |
| author_sort | Lei Li |
| collection | DOAJ |
| description | Exertional heat stroke (EHS) is a life-threatening condition characterized by hyperthermia and multi-organ dysfunction, often associated with intestinal barrier disruption. This study evaluated the protective effects of Huoxiang Zhengqi Dropping Pills (HXZQD) against EHS in a rat model. HXZQD was administered via oral gavage at low, medium, and high doses, followed by EHS induction through exercise under high-temperature and high-humidity conditions. The findings revealed that high-dose HXZQD significantly delayed the onset of EHS, reduced core body temperature elevations, and mitigated multi-organ injury, as evidenced by biochemical markers and histopathological examination. This study showed that HXZQD alleviated EHS-induced intestinal damage by preserving barrier proteins (ZO-1, Occludin, and Ecadherin) and maintaining intestinal barrier integrity. Transmission electron microscopy confirmed the preservation of tight junction structures. Further analysis indicated that HXZQD modulated the MAPK/NF-κB signaling pathways, inhibiting heat stress-induced activation and reducing inflammation. Additionally, HXZQD positively regulated gut microbiota, increasing the proportion of beneficial Lactococcus and decreasing harmful Streptococcus. These findings suggest that HXZQD maintains intestinal homeostasis during EHS by preserving barrier function and modulating gut microbiota, offering a promising preventive approach for EHS management. |
| format | Article |
| id | doaj-art-194211904f944ce09150f64a5d565822 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-194211904f944ce09150f64a5d5658222025-01-07T06:41:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15347131534713Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in ratsLei Li0Lei Li1Lei Li2Jun Ma3Jun Ma4Zeshi Li5Zeshi Li6Juelin Chen7Jiawei Zhou8Yawei Wang9Yankun Pei10Yitong Gong11Jianyao You12Yangyang Cao13Man Wang14Jikuai Chen15Wenjun Chang16Weiyi Ma17Hanyu Zhu18Chuhan Xiang19Shuogui Xu20Qing Song21Qing Song22Heat Stroke Treatment and Research Center of Chinese PLA General Hospital, Sanya, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, The Second Naval Hospital of Southern Theater Command of PLA, Sanya, ChinaHeat Stroke Treatment and Research Center of Chinese PLA General Hospital, Sanya, ChinaDepartment of Critical Care Medicine, Hainan Hospital, Chinese PLA General Hospital, Sanya, ChinaHeat Stroke Treatment and Research Center of Chinese PLA General Hospital, Sanya, ChinaDepartment of Critical Care Medicine, Hainan Hospital, Chinese PLA General Hospital, Sanya, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Emergency, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Rehabilitation and Traditional Chinese Medicine, Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Health Toxicology, Faculty of Naval Medicine, Naval Medical University, Shanghai, ChinaFaculty of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Emergency, First Medical Center of Chinese PLA General Hospital, Beijing, ChinaNational Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases Research, Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Beijing, China0Department of Cardiology, The Affiliated Huaihai Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, ChinaDepartment of Critical Care Medicine, Hainan Hospital, Chinese PLA General Hospital, Sanya, ChinaHeat Stroke Treatment and Research Center of Chinese PLA General Hospital, Sanya, ChinaDepartment of Critical Care Medicine, Hainan Hospital, Chinese PLA General Hospital, Sanya, ChinaExertional heat stroke (EHS) is a life-threatening condition characterized by hyperthermia and multi-organ dysfunction, often associated with intestinal barrier disruption. This study evaluated the protective effects of Huoxiang Zhengqi Dropping Pills (HXZQD) against EHS in a rat model. HXZQD was administered via oral gavage at low, medium, and high doses, followed by EHS induction through exercise under high-temperature and high-humidity conditions. The findings revealed that high-dose HXZQD significantly delayed the onset of EHS, reduced core body temperature elevations, and mitigated multi-organ injury, as evidenced by biochemical markers and histopathological examination. This study showed that HXZQD alleviated EHS-induced intestinal damage by preserving barrier proteins (ZO-1, Occludin, and Ecadherin) and maintaining intestinal barrier integrity. Transmission electron microscopy confirmed the preservation of tight junction structures. Further analysis indicated that HXZQD modulated the MAPK/NF-κB signaling pathways, inhibiting heat stress-induced activation and reducing inflammation. Additionally, HXZQD positively regulated gut microbiota, increasing the proportion of beneficial Lactococcus and decreasing harmful Streptococcus. These findings suggest that HXZQD maintains intestinal homeostasis during EHS by preserving barrier function and modulating gut microbiota, offering a promising preventive approach for EHS management.https://www.frontiersin.org/articles/10.3389/fphar.2024.1534713/fullexertional heat strokeheat stressheat tolerancedietary supplementintestinal barriergut microbiota |
| spellingShingle | Lei Li Lei Li Lei Li Jun Ma Jun Ma Zeshi Li Zeshi Li Juelin Chen Jiawei Zhou Yawei Wang Yankun Pei Yitong Gong Jianyao You Yangyang Cao Man Wang Jikuai Chen Wenjun Chang Weiyi Ma Hanyu Zhu Chuhan Xiang Shuogui Xu Qing Song Qing Song Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats Frontiers in Pharmacology exertional heat stroke heat stress heat tolerance dietary supplement intestinal barrier gut microbiota |
| title | Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats |
| title_full | Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats |
| title_fullStr | Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats |
| title_full_unstemmed | Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats |
| title_short | Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut microbiota in rats |
| title_sort | huoxiang zhengqi dropping pills alleviate exertional heat stroke induced multiple organ injury through sustaining intestinal homeostasis via regulating mapk nf κb pathway and gut microbiota in rats |
| topic | exertional heat stroke heat stress heat tolerance dietary supplement intestinal barrier gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1534713/full |
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