Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties
Abstract The production of value-added bioproducts from renewable carbon sources has gained increasing attention in recent years. The aim of this study was to develop economical approaches for the production of prebiotic XOS with defined degree of polymerization (DP) by Bacillus subtilis MR42 from c...
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| Format: | Article |
| Language: | English |
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BMC
2025-06-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-025-02756-3 |
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| author | Lusha Wei Shuo Sun Yuan Chen Xin An Hanning Wu Yifei Wu |
| author_facet | Lusha Wei Shuo Sun Yuan Chen Xin An Hanning Wu Yifei Wu |
| author_sort | Lusha Wei |
| collection | DOAJ |
| description | Abstract The production of value-added bioproducts from renewable carbon sources has gained increasing attention in recent years. The aim of this study was to develop economical approaches for the production of prebiotic XOS with defined degree of polymerization (DP) by Bacillus subtilis MR42 from corn stalks. The MR42 strain has been genetically modified to delete the xynC gene, which enables it to convert xylan into specific XOS without generating xylose as a byproduct. Under optimal conditions, a high yield of XOS (703.4 mg/g xylan) was obtained. The purified XOS were further characterized using various techniques including TLC, HPLC, ESI-MS and 1HNMR spectroscopy. The analysis revealed that the XOS primarily consisted of xylotriose (257 ± 0.14 mg/g XOS), xylotetraose (267 ± 0.24 mg/g XOS) and xylopentaose (289 ± 0.29 mg/g XOS), accounting for 81.3% of the total XOS. The prebiotic effect of XOS was evaluated by examining its impact on the growth of Lactobacillus, the production of short-chain fatty acids, and its antioxidant activity. B. subtilis MR42 showed a promising ability to produce XOS of DP 3–5, without the generation of xylose. |
| format | Article |
| id | doaj-art-193dc0a97fb640c08f72d6ce43aa7351 |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-193dc0a97fb640c08f72d6ce43aa73512025-08-20T03:47:16ZengBMCMicrobial Cell Factories1475-28592025-06-0124111410.1186/s12934-025-02756-3Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic propertiesLusha Wei0Shuo Sun1Yuan Chen2Xin An3Hanning Wu4Yifei Wu5College of Food Engineering and Nutritional Science, Shaanxi Normal UniversityCollege of Food Science and Technology, Northwest UniversityCollege of Food Science and Technology, Northwest UniversityCollege of Food Science and Technology, Northwest UniversityDepartment of Geology, Northwest UniversityCollege of Food Science and Technology, Northwest UniversityAbstract The production of value-added bioproducts from renewable carbon sources has gained increasing attention in recent years. The aim of this study was to develop economical approaches for the production of prebiotic XOS with defined degree of polymerization (DP) by Bacillus subtilis MR42 from corn stalks. The MR42 strain has been genetically modified to delete the xynC gene, which enables it to convert xylan into specific XOS without generating xylose as a byproduct. Under optimal conditions, a high yield of XOS (703.4 mg/g xylan) was obtained. The purified XOS were further characterized using various techniques including TLC, HPLC, ESI-MS and 1HNMR spectroscopy. The analysis revealed that the XOS primarily consisted of xylotriose (257 ± 0.14 mg/g XOS), xylotetraose (267 ± 0.24 mg/g XOS) and xylopentaose (289 ± 0.29 mg/g XOS), accounting for 81.3% of the total XOS. The prebiotic effect of XOS was evaluated by examining its impact on the growth of Lactobacillus, the production of short-chain fatty acids, and its antioxidant activity. B. subtilis MR42 showed a promising ability to produce XOS of DP 3–5, without the generation of xylose.https://doi.org/10.1186/s12934-025-02756-3XylooligosaccharidesBacillus subtilisCorn stalksLactobacillus |
| spellingShingle | Lusha Wei Shuo Sun Yuan Chen Xin An Hanning Wu Yifei Wu Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties Microbial Cell Factories Xylooligosaccharides Bacillus subtilis Corn stalks Lactobacillus |
| title | Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties |
| title_full | Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties |
| title_fullStr | Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties |
| title_full_unstemmed | Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties |
| title_short | Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties |
| title_sort | harnessing the power of bacillus subtilis mr42 direct production of defined xylooligosaccharides xos from corn stalks with enhanced prebiotic properties |
| topic | Xylooligosaccharides Bacillus subtilis Corn stalks Lactobacillus |
| url | https://doi.org/10.1186/s12934-025-02756-3 |
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