Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil
Introduction: Extraintestinal pathogenic Escherichia coli (ExPEC) is associated with various diseases such as urinary tract infections, neonatal meningitis and septicemia. There are many virulence factors (VF) encoded by genes in ExPEC, including papC, papG, ecpA, iroN, fyuA, iutA, ompTp, tsh, hlyF,...
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The Journal of Infection in Developing Countries
2015-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/6683 |
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| author | Paula Signolfi Cyoia Gabriela Regina Rodrigues Erick Kenji Nishio Leonardo Pinto Medeiros Vanessa Lumi Koga Ana Paula Dier Pereira Eliana Carolina Vespero Sébastien Houle Charles M Dozois Gerson Nakazato Renata K. T. Kobayashi |
| author_facet | Paula Signolfi Cyoia Gabriela Regina Rodrigues Erick Kenji Nishio Leonardo Pinto Medeiros Vanessa Lumi Koga Ana Paula Dier Pereira Eliana Carolina Vespero Sébastien Houle Charles M Dozois Gerson Nakazato Renata K. T. Kobayashi |
| author_sort | Paula Signolfi Cyoia |
| collection | DOAJ |
| description | Introduction: Extraintestinal pathogenic Escherichia coli (ExPEC) is associated with various diseases such as urinary tract infections, neonatal meningitis and septicemia. There are many virulence factors (VF) encoded by genes in ExPEC, including papC, papG, ecpA, iroN, fyuA, iutA, ompTp, tsh, hlyF, hlyA and iss. These virulence genes may be present in pathogenicity islands (PAI) or plasmids.
Methodology: In this study, we analyzed the presence of VF encoding genes, PAI sequences and phylogenetic groups of 96 ExPEC strains isolated from the urine and blood of patients at the University Hospital of Londrina, and we compared them with 50 faecal commensal strains from healthy individuals.
Results: The VF fyuA (65.60%) was detected in pathogenic strains and commensal strains (46%). A comparison of the distribution of ExPEC and commensal strains in the phylogenetic groups showed that more ExPEC strains belonged to group B2 whereas more of the commensal isolates belonged to group A. The distribution of the seven PAI sequences between commensal strains and ExPEC strains showed that PAI IV536 was common in both ExPEC and commensal isolates.
Conclusions: These results showed that the ExPEC strains that belonged to group B2 had more PAI sequences compared to those of the other groups, especially group B1, which had virulence genes but the lowest percentage of PAI sequences, which leads us to conclude that the virulence of ExPEC strains characterized as B2 is likely attributed to PAI encoded genes, whereas the virulence of ExPEC strains belonging to phylogenetic group B1 is likely due to plasmid encoded virulence genes.
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| format | Article |
| id | doaj-art-1933fedd90044c8ba99f92de9aaeeb57 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-1933fedd90044c8ba99f92de9aaeeb572025-08-20T02:27:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-10-0191010.3855/jidc.6683Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from BrazilPaula Signolfi Cyoia0Gabriela Regina Rodrigues1Erick Kenji Nishio2Leonardo Pinto Medeiros3Vanessa Lumi Koga4Ana Paula Dier Pereira5Eliana Carolina Vespero6Sébastien Houle7Charles M Dozois8Gerson Nakazato9Renata K. T. Kobayashi10Biological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilBiological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilBiological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilBiological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilBiological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilClinical Microbiology Laboratory, Clinical Analysis and Toxicological, University Hospital, Universidade Estadual de Londrina, Londrina, Paraná, BrazilClinical Microbiology Laboratory, Clinical Analysis and Toxicological, University Hospital, Universidade Estadual de Londrina, Londrina, Paraná, BrazilInstitut National de la Recherche Scientifique, Institut Armand-Frappier (INRS), Laval, Quebec, CanadaInstitut National de la Recherche Scientifique, Institut Armand-Frappier (INRS), Laval, Quebec, CanadaBiological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilBiological Sciences Center, Universidade Estadual de Londrina, Londrina, Paraná, BrazilIntroduction: Extraintestinal pathogenic Escherichia coli (ExPEC) is associated with various diseases such as urinary tract infections, neonatal meningitis and septicemia. There are many virulence factors (VF) encoded by genes in ExPEC, including papC, papG, ecpA, iroN, fyuA, iutA, ompTp, tsh, hlyF, hlyA and iss. These virulence genes may be present in pathogenicity islands (PAI) or plasmids. Methodology: In this study, we analyzed the presence of VF encoding genes, PAI sequences and phylogenetic groups of 96 ExPEC strains isolated from the urine and blood of patients at the University Hospital of Londrina, and we compared them with 50 faecal commensal strains from healthy individuals. Results: The VF fyuA (65.60%) was detected in pathogenic strains and commensal strains (46%). A comparison of the distribution of ExPEC and commensal strains in the phylogenetic groups showed that more ExPEC strains belonged to group B2 whereas more of the commensal isolates belonged to group A. The distribution of the seven PAI sequences between commensal strains and ExPEC strains showed that PAI IV536 was common in both ExPEC and commensal isolates. Conclusions: These results showed that the ExPEC strains that belonged to group B2 had more PAI sequences compared to those of the other groups, especially group B1, which had virulence genes but the lowest percentage of PAI sequences, which leads us to conclude that the virulence of ExPEC strains characterized as B2 is likely attributed to PAI encoded genes, whereas the virulence of ExPEC strains belonging to phylogenetic group B1 is likely due to plasmid encoded virulence genes. https://jidc.org/index.php/journal/article/view/6683ExPECcommensal Escherichia colivirulence factorsPAI sequencesphylogenetic groups |
| spellingShingle | Paula Signolfi Cyoia Gabriela Regina Rodrigues Erick Kenji Nishio Leonardo Pinto Medeiros Vanessa Lumi Koga Ana Paula Dier Pereira Eliana Carolina Vespero Sébastien Houle Charles M Dozois Gerson Nakazato Renata K. T. Kobayashi Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil Journal of Infection in Developing Countries ExPEC commensal Escherichia coli virulence factors PAI sequences phylogenetic groups |
| title | Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil |
| title_full | Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil |
| title_fullStr | Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil |
| title_full_unstemmed | Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil |
| title_short | Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil |
| title_sort | presence of virulence genes and pathogenicity islands in extraintestinal pathogenic escherichia coli isolates from brazil |
| topic | ExPEC commensal Escherichia coli virulence factors PAI sequences phylogenetic groups |
| url | https://jidc.org/index.php/journal/article/view/6683 |
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