DNA calorimetric force spectroscopy at single base pair resolution
Abstract DNA hybridization is a fundamental molecular reaction with wide-ranging applications in biotechnology. The knowledge of the temperature dependence of the thermodynamic parameters of duplex formation is crucial for quantitative predictions throughout the DNA stability range. It is commonly a...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-03-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57340-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849390308345774080 |
|---|---|
| author | P. Rissone M. Rico-Pasto S. B. Smith F. Ritort |
| author_facet | P. Rissone M. Rico-Pasto S. B. Smith F. Ritort |
| author_sort | P. Rissone |
| collection | DOAJ |
| description | Abstract DNA hybridization is a fundamental molecular reaction with wide-ranging applications in biotechnology. The knowledge of the temperature dependence of the thermodynamic parameters of duplex formation is crucial for quantitative predictions throughout the DNA stability range. It is commonly assumed that enthalpies and entropies are temperature independent, and heat capacity changes ΔC p equal zero. However, it has been known that this assumption is a poor approximation for a long time. Here, we combine single-DNA mechanical unzipping experiments using a temperature jump optical trap with a tailored statistical analysis to derive the ten heat-capacity change parameters of the nearest-neighbor model. Calorimetric force spectroscopy establishes a groundbreaking approach to studying nucleic acids that can be further extended to chemically modified DNA, RNA, and DNA/RNA hybrid structures. |
| format | Article |
| id | doaj-art-192de5857f0e4769b5a14367c448cc71 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-192de5857f0e4769b5a14367c448cc712025-08-20T03:41:42ZengNature PortfolioNature Communications2041-17232025-03-0116111010.1038/s41467-025-57340-5DNA calorimetric force spectroscopy at single base pair resolutionP. Rissone0M. Rico-Pasto1S. B. Smith2F. Ritort3Small Biosystems Lab, Condensed Matter Physics Departement, Universitat de BarcelonaUnit of Biophysics and Bioengineering, Department of Biomedicine, School of Medicine and Health Sciences, Universitat de BarcelonaSteven B. Smith EngineeringSmall Biosystems Lab, Condensed Matter Physics Departement, Universitat de BarcelonaAbstract DNA hybridization is a fundamental molecular reaction with wide-ranging applications in biotechnology. The knowledge of the temperature dependence of the thermodynamic parameters of duplex formation is crucial for quantitative predictions throughout the DNA stability range. It is commonly assumed that enthalpies and entropies are temperature independent, and heat capacity changes ΔC p equal zero. However, it has been known that this assumption is a poor approximation for a long time. Here, we combine single-DNA mechanical unzipping experiments using a temperature jump optical trap with a tailored statistical analysis to derive the ten heat-capacity change parameters of the nearest-neighbor model. Calorimetric force spectroscopy establishes a groundbreaking approach to studying nucleic acids that can be further extended to chemically modified DNA, RNA, and DNA/RNA hybrid structures.https://doi.org/10.1038/s41467-025-57340-5 |
| spellingShingle | P. Rissone M. Rico-Pasto S. B. Smith F. Ritort DNA calorimetric force spectroscopy at single base pair resolution Nature Communications |
| title | DNA calorimetric force spectroscopy at single base pair resolution |
| title_full | DNA calorimetric force spectroscopy at single base pair resolution |
| title_fullStr | DNA calorimetric force spectroscopy at single base pair resolution |
| title_full_unstemmed | DNA calorimetric force spectroscopy at single base pair resolution |
| title_short | DNA calorimetric force spectroscopy at single base pair resolution |
| title_sort | dna calorimetric force spectroscopy at single base pair resolution |
| url | https://doi.org/10.1038/s41467-025-57340-5 |
| work_keys_str_mv | AT prissone dnacalorimetricforcespectroscopyatsinglebasepairresolution AT mricopasto dnacalorimetricforcespectroscopyatsinglebasepairresolution AT sbsmith dnacalorimetricforcespectroscopyatsinglebasepairresolution AT fritort dnacalorimetricforcespectroscopyatsinglebasepairresolution |