Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats
An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically he...
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| Language: | English |
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Wiley
2016-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2016/2638371 |
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| author | Poonam Shakya A. K. Sharma Naveen Kumar Remya Vellachi Dayamon D. Mathew Prasoon Dubey Kiranjeet Singh Sonal Shrivastava Sameer Shrivastava S. K. Maiti Anwarul Hasan K. P. Singh |
| author_facet | Poonam Shakya A. K. Sharma Naveen Kumar Remya Vellachi Dayamon D. Mathew Prasoon Dubey Kiranjeet Singh Sonal Shrivastava Sameer Shrivastava S. K. Maiti Anwarul Hasan K. P. Singh |
| author_sort | Poonam Shakya |
| collection | DOAJ |
| description | An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats. |
| format | Article |
| id | doaj-art-192bb0a048704ebc8c770ab1369ff785 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-192bb0a048704ebc8c770ab1369ff7852025-08-20T03:55:45ZengWileyScientifica2090-908X2016-01-01201610.1155/2016/26383712638371Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in RatsPoonam Shakya0A. K. Sharma1Naveen Kumar2Remya Vellachi3Dayamon D. Mathew4Prasoon Dubey5Kiranjeet Singh6Sonal Shrivastava7Sameer Shrivastava8S. K. Maiti9Anwarul Hasan10K. P. Singh11Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaDivision of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaCentre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, IndiaAn acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.http://dx.doi.org/10.1155/2016/2638371 |
| spellingShingle | Poonam Shakya A. K. Sharma Naveen Kumar Remya Vellachi Dayamon D. Mathew Prasoon Dubey Kiranjeet Singh Sonal Shrivastava Sameer Shrivastava S. K. Maiti Anwarul Hasan K. P. Singh Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats Scientifica |
| title | Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats |
| title_full | Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats |
| title_fullStr | Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats |
| title_full_unstemmed | Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats |
| title_short | Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats |
| title_sort | bubaline cholecyst derived extracellular matrix for reconstruction of full thickness skin wounds in rats |
| url | http://dx.doi.org/10.1155/2016/2638371 |
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