Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock
Water temperature significantly affects the physiological balance of aquatic organisms like crustaceans, and heat shock proteins (HSPs) are crucial for stress resistance and pathogen defense. This study conducted a genome-wide analysis to explore the functional characteristics of the <i>Hsp70&...
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2025-07-01
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| author | Xin Zhang Xiuhong Cai Shirui Yue Zhangxuan Chen Yulong Sun Lei Cheng Yewen Xi Shunchang Wang |
| author_facet | Xin Zhang Xiuhong Cai Shirui Yue Zhangxuan Chen Yulong Sun Lei Cheng Yewen Xi Shunchang Wang |
| author_sort | Xin Zhang |
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| description | Water temperature significantly affects the physiological balance of aquatic organisms like crustaceans, and heat shock proteins (HSPs) are crucial for stress resistance and pathogen defense. This study conducted a genome-wide analysis to explore the functional characteristics of the <i>Hsp70</i> gene family in <i>Procambarus clarkii</i>. Fifteen <i>Hsp70</i> family members were identified, with several genes showing upregulation under non-lethal heat shock (NLHS) and pathogen challenges. RNA-Seq and qPCR analyses confirmed increased expression of certain <i>PcHsp70s</i> during NLHS, indicating NLHS activation of the <i>Hsp70</i> family to enhance immune regulation. dsRNA-mediated silencing of <i>Hsp70</i> led to downregulation of TLR pathway genes (e.g., <i>TLR1</i>, <i>TLR6</i>), suggesting <i>Hsp70</i> regulates the TLR signaling pathway for immune responses. These findings reveal that NLHS-induced <i>Hsp70</i> upregulation improves pathogen resistance, offering insights for addressing temperature fluctuations and disease outbreaks in aquaculture to optimize management practices. |
| format | Article |
| id | doaj-art-192abfa85434457ebd061184582e5e18 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-192abfa85434457ebd061184582e5e182025-08-20T03:55:48ZengMDPI AGAnimals2076-26152025-07-011514215010.3390/ani15142150Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat ShockXin Zhang0Xiuhong Cai1Shirui Yue2Zhangxuan Chen3Yulong Sun4Lei Cheng5Yewen Xi6Shunchang Wang7School of Biological Engineering, Huainan Normal University, Huainan 232001, ChinaSchool of Biological Engineering, Huainan Normal University, Huainan 232001, ChinaSchool of Biological Engineering, Huainan Normal University, Huainan 232001, ChinaSchool of Biological Engineering, Huainan Normal University, Huainan 232001, ChinaDepartment of Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Biological Engineering, Huainan Normal University, Huainan 232001, ChinaAnhui Provincial General Station of Aquatic Technology Extension, Hefei 230091, ChinaSchool of Biological Engineering, Huainan Normal University, Huainan 232001, ChinaWater temperature significantly affects the physiological balance of aquatic organisms like crustaceans, and heat shock proteins (HSPs) are crucial for stress resistance and pathogen defense. This study conducted a genome-wide analysis to explore the functional characteristics of the <i>Hsp70</i> gene family in <i>Procambarus clarkii</i>. Fifteen <i>Hsp70</i> family members were identified, with several genes showing upregulation under non-lethal heat shock (NLHS) and pathogen challenges. RNA-Seq and qPCR analyses confirmed increased expression of certain <i>PcHsp70s</i> during NLHS, indicating NLHS activation of the <i>Hsp70</i> family to enhance immune regulation. dsRNA-mediated silencing of <i>Hsp70</i> led to downregulation of TLR pathway genes (e.g., <i>TLR1</i>, <i>TLR6</i>), suggesting <i>Hsp70</i> regulates the TLR signaling pathway for immune responses. These findings reveal that NLHS-induced <i>Hsp70</i> upregulation improves pathogen resistance, offering insights for addressing temperature fluctuations and disease outbreaks in aquaculture to optimize management practices.https://www.mdpi.com/2076-2615/15/14/2150non-lethal heat shock<i>Hsp70</i> gene familyimmune regulation<i>Procambarus clarkii</i> |
| spellingShingle | Xin Zhang Xiuhong Cai Shirui Yue Zhangxuan Chen Yulong Sun Lei Cheng Yewen Xi Shunchang Wang Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock Animals non-lethal heat shock <i>Hsp70</i> gene family immune regulation <i>Procambarus clarkii</i> |
| title | Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock |
| title_full | Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock |
| title_fullStr | Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock |
| title_short | Genome-Wide Identification and Expression Analysis of <i>Hsp70</i> Gene Family of <i>Procambarus clarkii</i> Reveals Its Immune Role in Response to Bacterial Challenge After Non-Lethal Heat Shock |
| title_sort | genome wide identification and expression analysis of i hsp70 i gene family of i procambarus clarkii i reveals its immune role in response to bacterial challenge after non lethal heat shock |
| topic | non-lethal heat shock <i>Hsp70</i> gene family immune regulation <i>Procambarus clarkii</i> |
| url | https://www.mdpi.com/2076-2615/15/14/2150 |
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