Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa
ABSTRACT The single polar flagellar motor in Pseudomonas aeruginosa is equipped with two stator systems, MotAB and MotCD, both driven by H+ ions. The torque-speed relationship for flagellar motors with two stator systems has not been explored previously. In this study, we developed a method that uti...
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American Society for Microbiology
2024-12-01
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| Series: | mBio |
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| Online Access: | https://journals.asm.org/doi/10.1128/mbio.00745-24 |
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| author | Haolin Wu Zhengyu Wu Maojin Tian Rongjing Zhang Junhua Yuan |
| author_facet | Haolin Wu Zhengyu Wu Maojin Tian Rongjing Zhang Junhua Yuan |
| author_sort | Haolin Wu |
| collection | DOAJ |
| description | ABSTRACT The single polar flagellar motor in Pseudomonas aeruginosa is equipped with two stator systems, MotAB and MotCD, both driven by H+ ions. The torque-speed relationship for flagellar motors with two stator systems has not been explored previously. In this study, we developed a method that utilizes optical trapping and fluorescence labeling to measure the torque-speed relationships for the wild-type P. aeruginosa motor with dual stators and mutant strains with a single stator system, revealing surprising differences in them. Moreover, we found that the MotAB stators exhibit slip-bond behavior in load dependence, contrasting with the catch-bond behavior of the MotCD stators and Escherichia coli stators. Further examination of the solvent isotope and pH effects on the torque-speed relationships of these stator systems provided additional insights into their dynamics. Interestingly, we discovered that the torque of the wild-type motor is similar to the combined torque of motors with MotAB or MotCD stators, indicating an additive contribution from the two stator types in the wild-type motors. These findings underscore the enhanced adaptability of P. aeruginosa to a wide range of external environments with varying load conditions.IMPORTANCEWe developed a novel method to measure the flagellar motor torque-speed relationship by trapping a swimming bacterium using optical tweezers. Using the P. aeruginosa flagellar motor as a model system to investigate motor dynamics with dual stator types, we measured the torque-speed relationships for wild-type motors with dual stator types and mutants with a single type. We found drastic differences that stem from the varying load dependencies of stator stability. These variations enable bacteria to rapidly adjust their stator composition in response to external load conditions. Interestingly, we observed that the torque of the wild-type motor is akin to the cumulative torque of motors with either stator type, indicating an additive contribution from the two stator types in wild-type motors. The methodology we established here can be readily employed to study motor dynamics in other flagellated bacteria. |
| format | Article |
| id | doaj-art-1925c75afa1649929add7eae584bdee4 |
| institution | OA Journals |
| issn | 2150-7511 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj-art-1925c75afa1649929add7eae584bdee42025-08-20T01:58:59ZengAmerican Society for MicrobiologymBio2150-75112024-12-01151210.1128/mbio.00745-24Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosaHaolin Wu0Zhengyu Wu1Maojin Tian2Rongjing Zhang3Junhua Yuan4Hefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of China, Hefei, Anhui, ChinaHefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of China, Hefei, Anhui, ChinaCenter of Translational Medicine, Zibo Central Hospital Affiliated to Binzhou Medical University, Zibo, Shandong, ChinaHefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of China, Hefei, Anhui, ChinaHefei National Research Center for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of China, Hefei, Anhui, ChinaABSTRACT The single polar flagellar motor in Pseudomonas aeruginosa is equipped with two stator systems, MotAB and MotCD, both driven by H+ ions. The torque-speed relationship for flagellar motors with two stator systems has not been explored previously. In this study, we developed a method that utilizes optical trapping and fluorescence labeling to measure the torque-speed relationships for the wild-type P. aeruginosa motor with dual stators and mutant strains with a single stator system, revealing surprising differences in them. Moreover, we found that the MotAB stators exhibit slip-bond behavior in load dependence, contrasting with the catch-bond behavior of the MotCD stators and Escherichia coli stators. Further examination of the solvent isotope and pH effects on the torque-speed relationships of these stator systems provided additional insights into their dynamics. Interestingly, we discovered that the torque of the wild-type motor is similar to the combined torque of motors with MotAB or MotCD stators, indicating an additive contribution from the two stator types in the wild-type motors. These findings underscore the enhanced adaptability of P. aeruginosa to a wide range of external environments with varying load conditions.IMPORTANCEWe developed a novel method to measure the flagellar motor torque-speed relationship by trapping a swimming bacterium using optical tweezers. Using the P. aeruginosa flagellar motor as a model system to investigate motor dynamics with dual stator types, we measured the torque-speed relationships for wild-type motors with dual stator types and mutants with a single type. We found drastic differences that stem from the varying load dependencies of stator stability. These variations enable bacteria to rapidly adjust their stator composition in response to external load conditions. Interestingly, we observed that the torque of the wild-type motor is akin to the cumulative torque of motors with either stator type, indicating an additive contribution from the two stator types in wild-type motors. The methodology we established here can be readily employed to study motor dynamics in other flagellated bacteria.https://journals.asm.org/doi/10.1128/mbio.00745-24optical tweezersbacterial motilitybacterial flagellar motorload dependenceslip bondcatch bond |
| spellingShingle | Haolin Wu Zhengyu Wu Maojin Tian Rongjing Zhang Junhua Yuan Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa mBio optical tweezers bacterial motility bacterial flagellar motor load dependence slip bond catch bond |
| title | Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa |
| title_full | Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa |
| title_fullStr | Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa |
| title_full_unstemmed | Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa |
| title_short | Torque-speed relationship of the flagellar motor with dual-stator systems in Pseudomonas aeruginosa |
| title_sort | torque speed relationship of the flagellar motor with dual stator systems in pseudomonas aeruginosa |
| topic | optical tweezers bacterial motility bacterial flagellar motor load dependence slip bond catch bond |
| url | https://journals.asm.org/doi/10.1128/mbio.00745-24 |
| work_keys_str_mv | AT haolinwu torquespeedrelationshipoftheflagellarmotorwithdualstatorsystemsinpseudomonasaeruginosa AT zhengyuwu torquespeedrelationshipoftheflagellarmotorwithdualstatorsystemsinpseudomonasaeruginosa AT maojintian torquespeedrelationshipoftheflagellarmotorwithdualstatorsystemsinpseudomonasaeruginosa AT rongjingzhang torquespeedrelationshipoftheflagellarmotorwithdualstatorsystemsinpseudomonasaeruginosa AT junhuayuan torquespeedrelationshipoftheflagellarmotorwithdualstatorsystemsinpseudomonasaeruginosa |