Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats
Background. Pedalium murex (P. murex) is used in folk medicine for treatment of male infertility. However, scientific data on its safety are limited. Objective. This study was carried out to assess the acute and repeated dose 28-day oral toxicity of the aqueous extracts from P. murex leafy stem and...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2023/2962905 |
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| author | Gérard Bessan Dossou-Agoin Maxime Machioud Sangaré-Oumar Téniola Isabelle Sacramento Mariette Sindété Egnon Jacques Hougbénou-Houngla Nounagnon Darius Tossavi Simon Azonbakin Adam Gbankoto |
| author_facet | Gérard Bessan Dossou-Agoin Maxime Machioud Sangaré-Oumar Téniola Isabelle Sacramento Mariette Sindété Egnon Jacques Hougbénou-Houngla Nounagnon Darius Tossavi Simon Azonbakin Adam Gbankoto |
| author_sort | Gérard Bessan Dossou-Agoin |
| collection | DOAJ |
| description | Background. Pedalium murex (P. murex) is used in folk medicine for treatment of male infertility. However, scientific data on its safety are limited. Objective. This study was carried out to assess the acute and repeated dose 28-day oral toxicity of the aqueous extracts from P. murex leafy stem and fruit in Wistar rats. Methods. The acute toxicity test was performed according to the line 423 of the Organization for Economic Cooperation and Development (OECD) guidelines. The rats were randomly divided into three groups (n = 3). The control group received distilled water, while the experimental groups were given at a single dose, 5000 mg/kg of each extract. The repeated dose 28-day oral toxicity was performed according to the line 407 of the OECD guidelines. 35 rats divided into 7 groups of 5 male rats each were daily treated for 28 days with each extract at 200 mg/kg, 400 mg/kg, and 800 mg/kg, respectively. The in-life parameters were recorded during the follow-up. At the end of this study, organ weights, hematology, biochemistry, and histology parameters were analyzed. Results. In the acute oral toxicity test, there was no morbidity or mortality related to the treatments. Both extracts belong therefore to category 5 of the globally harmonized system (GHS) of classification. In the repeated dose 28-day oral toxicity test, both extracts did not alter animal’s behavior. However, both extract administration led to proteinuria and renal damages. Conclusion. P. murex leafy stem and fruit aqueous extracts exhibited potential nephrotoxicity. Therefore, care should be taken when they are used over an extended period. |
| format | Article |
| id | doaj-art-1910cab5a11a4f4383960304be4a7bbd |
| institution | Kabale University |
| issn | 1687-8205 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-1910cab5a11a4f4383960304be4a7bbd2025-02-03T01:29:28ZengWileyJournal of Toxicology1687-82052023-01-01202310.1155/2023/2962905Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar RatsGérard Bessan Dossou-Agoin0Maxime Machioud Sangaré-Oumar1Téniola Isabelle Sacramento2Mariette Sindété3Egnon Jacques Hougbénou-Houngla4Nounagnon Darius Tossavi5Simon Azonbakin6Adam Gbankoto7Experimental Physiology and Pharmacology LaboratoryExperimental Physiology and Pharmacology LaboratorySchool of Management and Operation of Livestock SystemsExperimental Physiology and Pharmacology LaboratoryExperimental Physiology and Pharmacology LaboratoryExperimental Physiology and Pharmacology LaboratoryHistologyExperimental Physiology and Pharmacology LaboratoryBackground. Pedalium murex (P. murex) is used in folk medicine for treatment of male infertility. However, scientific data on its safety are limited. Objective. This study was carried out to assess the acute and repeated dose 28-day oral toxicity of the aqueous extracts from P. murex leafy stem and fruit in Wistar rats. Methods. The acute toxicity test was performed according to the line 423 of the Organization for Economic Cooperation and Development (OECD) guidelines. The rats were randomly divided into three groups (n = 3). The control group received distilled water, while the experimental groups were given at a single dose, 5000 mg/kg of each extract. The repeated dose 28-day oral toxicity was performed according to the line 407 of the OECD guidelines. 35 rats divided into 7 groups of 5 male rats each were daily treated for 28 days with each extract at 200 mg/kg, 400 mg/kg, and 800 mg/kg, respectively. The in-life parameters were recorded during the follow-up. At the end of this study, organ weights, hematology, biochemistry, and histology parameters were analyzed. Results. In the acute oral toxicity test, there was no morbidity or mortality related to the treatments. Both extracts belong therefore to category 5 of the globally harmonized system (GHS) of classification. In the repeated dose 28-day oral toxicity test, both extracts did not alter animal’s behavior. However, both extract administration led to proteinuria and renal damages. Conclusion. P. murex leafy stem and fruit aqueous extracts exhibited potential nephrotoxicity. Therefore, care should be taken when they are used over an extended period.http://dx.doi.org/10.1155/2023/2962905 |
| spellingShingle | Gérard Bessan Dossou-Agoin Maxime Machioud Sangaré-Oumar Téniola Isabelle Sacramento Mariette Sindété Egnon Jacques Hougbénou-Houngla Nounagnon Darius Tossavi Simon Azonbakin Adam Gbankoto Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats Journal of Toxicology |
| title | Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats |
| title_full | Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats |
| title_fullStr | Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats |
| title_full_unstemmed | Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats |
| title_short | Acute and Repeated Dose 28-Day Oral Toxicity Study of the Aqueous Extracts from the Leafy Stem and Fruit of Pedalium murex D.Royen EX.L in Wistar Rats |
| title_sort | acute and repeated dose 28 day oral toxicity study of the aqueous extracts from the leafy stem and fruit of pedalium murex d royen ex l in wistar rats |
| url | http://dx.doi.org/10.1155/2023/2962905 |
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