Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools

This study develops a green and sustainable analytical method using cellulose paper sorptive extraction (CPSE) coupled with gas chromatography-mass spectrometry (GC–MS) to determine dextromethorphan, tramadol, clozapine, and zolpidem (Ambien) in urine. The method involves immersing five 1″ × 1″ cell...

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Main Authors: Torki A. Zughaibi, Ahmed I. Al-Asmari
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Green Analytical Chemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772577424000879
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author Torki A. Zughaibi
Ahmed I. Al-Asmari
author_facet Torki A. Zughaibi
Ahmed I. Al-Asmari
author_sort Torki A. Zughaibi
collection DOAJ
description This study develops a green and sustainable analytical method using cellulose paper sorptive extraction (CPSE) coupled with gas chromatography-mass spectrometry (GC–MS) to determine dextromethorphan, tramadol, clozapine, and zolpidem (Ambien) in urine. The method involves immersing five 1″ × 1″ cellulose papers in diluted urine (0.5 mL urine diluted to 10 mL) to adsorb the drugs at 250 rpm for 45 min, followed by desorption with 2 mL methanol at 200 rpm for 10 min and subsequent GC–MS analysis. Key parameters, including the number and size of filter papers, elution solvent type and volume, extraction and elution times and speeds, sample pH, and ionic strength, were extensively optimized. Validation according to SWGTOX guidelines demonstrated excellent linearity (0.05–1 µg mL-1, R² > 0.999), high precision (RSD < 11.1 %), and accuracy (85.1–113.6 %). Sensitivity was high, with LODs ranging from 0.010- 0.013 µg mL-1 and LOQs from 0.033 to 0.043 µg mL-1. The method was successfully applied to blind urine samples, confirming its practical utility in forensic toxicology. Greenness assessment using ComplexMoGAPI and practicality evaluation using Blue Applicability Grade Index (BAGI) yielded high scores (81 and 70), underscoring the method's environmental sustainability and fitness for purpose, positioning it as an eco-friendly alternative to conventional extraction methods.
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spelling doaj-art-190ea58c1c2f462eb4784b42237d943d2025-08-20T02:50:16ZengElsevierGreen Analytical Chemistry2772-57742025-03-011210017810.1016/j.greeac.2024.100178Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI toolsTorki A. Zughaibi0Ahmed I. Al-Asmari1Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia; King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia; Corresponding author.Special Toxicological Analysis Section, Pathology and Laboratory Medicine Department, King Faisal Specialist Hospital and Research CenterThis study develops a green and sustainable analytical method using cellulose paper sorptive extraction (CPSE) coupled with gas chromatography-mass spectrometry (GC–MS) to determine dextromethorphan, tramadol, clozapine, and zolpidem (Ambien) in urine. The method involves immersing five 1″ × 1″ cellulose papers in diluted urine (0.5 mL urine diluted to 10 mL) to adsorb the drugs at 250 rpm for 45 min, followed by desorption with 2 mL methanol at 200 rpm for 10 min and subsequent GC–MS analysis. Key parameters, including the number and size of filter papers, elution solvent type and volume, extraction and elution times and speeds, sample pH, and ionic strength, were extensively optimized. Validation according to SWGTOX guidelines demonstrated excellent linearity (0.05–1 µg mL-1, R² > 0.999), high precision (RSD < 11.1 %), and accuracy (85.1–113.6 %). Sensitivity was high, with LODs ranging from 0.010- 0.013 µg mL-1 and LOQs from 0.033 to 0.043 µg mL-1. The method was successfully applied to blind urine samples, confirming its practical utility in forensic toxicology. Greenness assessment using ComplexMoGAPI and practicality evaluation using Blue Applicability Grade Index (BAGI) yielded high scores (81 and 70), underscoring the method's environmental sustainability and fitness for purpose, positioning it as an eco-friendly alternative to conventional extraction methods.http://www.sciencedirect.com/science/article/pii/S2772577424000879Cellulose paper sorptive extraction (CPSE)Green sample preparationForensic drug analysisGreen analytical chemistry
spellingShingle Torki A. Zughaibi
Ahmed I. Al-Asmari
Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools
Green Analytical Chemistry
Cellulose paper sorptive extraction (CPSE)
Green sample preparation
Forensic drug analysis
Green analytical chemistry
title Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools
title_full Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools
title_fullStr Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools
title_full_unstemmed Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools
title_short Study of adsorption potential of cellulose paper for abusable prescription drug analysis: A practical and sustainable approach validated by BAGI and ComplexMoGAPI tools
title_sort study of adsorption potential of cellulose paper for abusable prescription drug analysis a practical and sustainable approach validated by bagi and complexmogapi tools
topic Cellulose paper sorptive extraction (CPSE)
Green sample preparation
Forensic drug analysis
Green analytical chemistry
url http://www.sciencedirect.com/science/article/pii/S2772577424000879
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