Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family

Transposable elements (TEs) have the ability to move and amplify inside the host genome, making them a pivotal source of genome plasticity. Presently, only 4 TE clades (all classified as Class I retrotransposons) have been identified in trypanosomatids. We predicted repeat content and manually curat...

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Main Authors: Percy Omar Tullume-Vergara, Adriana Ludwig, Vyacheslav Yurchenko, Adriano Cappellazzo Coelho, Elizabeth Magiolo Coser, Marco Aurélio Krieger, Marta M.G. Teixeira, Jeffrey Jon Shaw, Joao M.P. Alves
Format: Article
Language:English
Published: Cambridge University Press
Series:Parasitology
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Online Access:https://www.cambridge.org/core/product/identifier/S0031182025100231/type/journal_article
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author Percy Omar Tullume-Vergara
Adriana Ludwig
Vyacheslav Yurchenko
Adriano Cappellazzo Coelho
Elizabeth Magiolo Coser
Marco Aurélio Krieger
Marta M.G. Teixeira
Jeffrey Jon Shaw
Joao M.P. Alves
author_facet Percy Omar Tullume-Vergara
Adriana Ludwig
Vyacheslav Yurchenko
Adriano Cappellazzo Coelho
Elizabeth Magiolo Coser
Marco Aurélio Krieger
Marta M.G. Teixeira
Jeffrey Jon Shaw
Joao M.P. Alves
author_sort Percy Omar Tullume-Vergara
collection DOAJ
description Transposable elements (TEs) have the ability to move and amplify inside the host genome, making them a pivotal source of genome plasticity. Presently, only 4 TE clades (all classified as Class I retrotransposons) have been identified in trypanosomatids. We predicted repeat content and manually curated TEs across the genomes of 57 trypanosomatids, shedding light on their proportions, diversity and dynamics. Our analysis yielded 214 TE consensus sequence models across the dataset, with abundance ranging from 0.1% to 7.2%. We found evidence of recent transposon activity in most species, with notable bursts in the Vickermania, Lafontella, Porcisia and Angomonas spp., along with Leishmania (Mundinia) chancei, L. (M.) orientalis and L. (M.) procaviensis. We confirmed that the 4 TE clades have colonized virtually all lineages of trypanosomatids, potentially playing a role in shaping their genome architecture. The effort of this work culminated in the establishment of the Trypanosomatid TE Database 1.0, a resource designed to standardize the TE annotation process that can serve as a foundation for future studies on trypanosomatid TEs.
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series Parasitology
spelling doaj-art-19078d41b5224c689bbfeee3726d5c1b2025-08-20T03:21:16ZengCambridge University PressParasitology0031-18201469-816111610.1017/S0031182025100231Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae familyPercy Omar Tullume-Vergara0Adriana Ludwig1Vyacheslav Yurchenko2https://orcid.org/0000-0003-4765-3263Adriano Cappellazzo Coelho3Elizabeth Magiolo Coser4Marco Aurélio Krieger5Marta M.G. Teixeira6Jeffrey Jon Shaw7Joao M.P. Alves8https://orcid.org/0000-0001-5914-2087Department of Parasitology, Institute for Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilDepartment of Evolution, Ecology and Behaviour, University of Liverpool, Liverpool, UKLife Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech RepublicDepartamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, BrazilDepartamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, BrazilVice Presidency of Production and Innovation in Health (VPPIS), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, BrazilDepartment of Parasitology, Institute for Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilDepartment of Parasitology, Institute for Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilDepartment of Parasitology, Institute for Biomedical Sciences, University of Sao Paulo, Sao Paulo, BrazilTransposable elements (TEs) have the ability to move and amplify inside the host genome, making them a pivotal source of genome plasticity. Presently, only 4 TE clades (all classified as Class I retrotransposons) have been identified in trypanosomatids. We predicted repeat content and manually curated TEs across the genomes of 57 trypanosomatids, shedding light on their proportions, diversity and dynamics. Our analysis yielded 214 TE consensus sequence models across the dataset, with abundance ranging from 0.1% to 7.2%. We found evidence of recent transposon activity in most species, with notable bursts in the Vickermania, Lafontella, Porcisia and Angomonas spp., along with Leishmania (Mundinia) chancei, L. (M.) orientalis and L. (M.) procaviensis. We confirmed that the 4 TE clades have colonized virtually all lineages of trypanosomatids, potentially playing a role in shaping their genome architecture. The effort of this work culminated in the establishment of the Trypanosomatid TE Database 1.0, a resource designed to standardize the TE annotation process that can serve as a foundation for future studies on trypanosomatid TEs.https://www.cambridge.org/core/product/identifier/S0031182025100231/type/journal_articleCREINGImobilomeSLACSTATEtransposable elementstrypanosomatidsVIPER
spellingShingle Percy Omar Tullume-Vergara
Adriana Ludwig
Vyacheslav Yurchenko
Adriano Cappellazzo Coelho
Elizabeth Magiolo Coser
Marco Aurélio Krieger
Marta M.G. Teixeira
Jeffrey Jon Shaw
Joao M.P. Alves
Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family
Parasitology
CRE
INGI
mobilome
SLACS
TATE
transposable elements
trypanosomatids
VIPER
title Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family
title_full Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family
title_fullStr Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family
title_full_unstemmed Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family
title_short Comparative analysis of the mobilome yields new insights into its diversity, dynamics and evolution in parasites of the Trypanosomatidae family
title_sort comparative analysis of the mobilome yields new insights into its diversity dynamics and evolution in parasites of the trypanosomatidae family
topic CRE
INGI
mobilome
SLACS
TATE
transposable elements
trypanosomatids
VIPER
url https://www.cambridge.org/core/product/identifier/S0031182025100231/type/journal_article
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