Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry
Escherichia coli is commensal bacteria in the intestinal of broiler chickens with low virulence. However, mostly pathogenic and cause colibacillosis illness. Inappropriate use of antibiotics in broiler chickens has been widely reported. This research aims to determine the presence of E. coli contami...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Indonesian |
| Published: |
Universitas Gadjah Mada
2024-12-01
|
| Series: | Jurnal Sain Veteriner |
| Online Access: | https://jurnal.ugm.ac.id/jsv/article/view/92624 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594192354770944 |
|---|---|
| author | Fatia Nasyna Afifa Kantamaryana Roostita L Balia Wendry Setiyadi Putranto |
| author_facet | Fatia Nasyna Afifa Kantamaryana Roostita L Balia Wendry Setiyadi Putranto |
| author_sort | Fatia Nasyna Afifa Kantamaryana |
| collection | DOAJ |
| description | Escherichia coli is commensal bacteria in the intestinal of broiler chickens with low virulence. However, mostly pathogenic and cause colibacillosis illness. Inappropriate use of antibiotics in broiler chickens has been widely reported. This research aims to determine the presence of E. coli contamination in the poultry environment and determine the levels of antibiotics resistance of erythromycin, doxycycline, oxytetracycline and neomycin. The research was carried out through isolation and identification of E. coli using EMB media, Gram staining, and biochemical tests. Then, an antibiotic resistance test was carried out using by Kirby-Bauer method. From a total of 10 samples (3 coops), 7 (70%) were positive for E. coli. The positive of 7 which for E. coli, 43% of the isolates came from fresh feces obtained from coops 1, 2, and 3; 43% of the isolates came from litter from coops 1, 2, and 3; and 14% of isolates from soil outside the coop 3. The antibiotic resistance results by Kirby-Bauer method showed that E. coli was 100% resistant to erythromycin, 100% to neomycin, 66.7% to doxycycline, and 66.7% to oxytetracycline throughout isolates from feces and litter. Other results showed that isolates from soil outside coop 3 showed resistance to all antibiotics. Taken together, we concluded the E. coli has been contamination which is proven by antibiotic resistance. |
| format | Article |
| id | doaj-art-1903e80abc864cddbc66d23d7ab530c3 |
| institution | Kabale University |
| issn | 2407-3733 |
| language | Indonesian |
| publishDate | 2024-12-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Sain Veteriner |
| spelling | doaj-art-1903e80abc864cddbc66d23d7ab530c32025-01-20T02:12:10ZindUniversitas Gadjah MadaJurnal Sain Veteriner2407-37332024-12-0142331732610.22146/jsv.9262436140Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler PoultryFatia Nasyna Afifa Kantamaryana0Roostita L Balia1Wendry Setiyadi Putranto2Pajadjaran UniversityPadjadjaran UniversityPadjadjaran UniversityEscherichia coli is commensal bacteria in the intestinal of broiler chickens with low virulence. However, mostly pathogenic and cause colibacillosis illness. Inappropriate use of antibiotics in broiler chickens has been widely reported. This research aims to determine the presence of E. coli contamination in the poultry environment and determine the levels of antibiotics resistance of erythromycin, doxycycline, oxytetracycline and neomycin. The research was carried out through isolation and identification of E. coli using EMB media, Gram staining, and biochemical tests. Then, an antibiotic resistance test was carried out using by Kirby-Bauer method. From a total of 10 samples (3 coops), 7 (70%) were positive for E. coli. The positive of 7 which for E. coli, 43% of the isolates came from fresh feces obtained from coops 1, 2, and 3; 43% of the isolates came from litter from coops 1, 2, and 3; and 14% of isolates from soil outside the coop 3. The antibiotic resistance results by Kirby-Bauer method showed that E. coli was 100% resistant to erythromycin, 100% to neomycin, 66.7% to doxycycline, and 66.7% to oxytetracycline throughout isolates from feces and litter. Other results showed that isolates from soil outside coop 3 showed resistance to all antibiotics. Taken together, we concluded the E. coli has been contamination which is proven by antibiotic resistance.https://jurnal.ugm.ac.id/jsv/article/view/92624 |
| spellingShingle | Fatia Nasyna Afifa Kantamaryana Roostita L Balia Wendry Setiyadi Putranto Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry Jurnal Sain Veteriner |
| title | Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry |
| title_full | Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry |
| title_fullStr | Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry |
| title_full_unstemmed | Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry |
| title_short | Isolation, Identification, and Resistance Test of Escherichia coli to Antibiotics in Ujung Berung Broiler Poultry |
| title_sort | isolation identification and resistance test of escherichia coli to antibiotics in ujung berung broiler poultry |
| url | https://jurnal.ugm.ac.id/jsv/article/view/92624 |
| work_keys_str_mv | AT fatianasynaafifakantamaryana isolationidentificationandresistancetestofescherichiacolitoantibioticsinujungberungbroilerpoultry AT roostitalbalia isolationidentificationandresistancetestofescherichiacolitoantibioticsinujungberungbroilerpoultry AT wendrysetiyadiputranto isolationidentificationandresistancetestofescherichiacolitoantibioticsinujungberungbroilerpoultry |