Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients
BackgroundClinically diagnosed pulmonary tuberculosis (TB) (CDPTB) patients account for a huge proportion of TB. However, little is known about the genetic diversity and drug resistance profile of Mycobacterium tuberculosis Complex (MTBC) strains in this group of patients.MethodUnmatched case–contro...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-12-01
|
| Series: | Frontiers in Public Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2024.1420685/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850058170963066880 |
|---|---|
| author | Alem Alemayehu Alem Alemayehu Alem Alemayehu Liya Wassie Dawit Hailu Alemayehu Bethlehem Adnew Sebsib Neway Dessalegn Abeje Tefera Sosina Ayalew Elena Hailu Samuel Ayele Berhanu Seyoum Kidist Bobosha Markos Abebe Abraham Aseffa Beyene Petros Rawleigh Howe |
| author_facet | Alem Alemayehu Alem Alemayehu Alem Alemayehu Liya Wassie Dawit Hailu Alemayehu Bethlehem Adnew Sebsib Neway Dessalegn Abeje Tefera Sosina Ayalew Elena Hailu Samuel Ayele Berhanu Seyoum Kidist Bobosha Markos Abebe Abraham Aseffa Beyene Petros Rawleigh Howe |
| author_sort | Alem Alemayehu |
| collection | DOAJ |
| description | BackgroundClinically diagnosed pulmonary tuberculosis (TB) (CDPTB) patients account for a huge proportion of TB. However, little is known about the genetic diversity and drug resistance profile of Mycobacterium tuberculosis Complex (MTBC) strains in this group of patients.MethodUnmatched case–control study was conducted among 313 PTB patients to compare the genetic diversity of MTBC and their drug resistance profiles among CDPTB (n = 173) and bacteriologically confirmed pulmonary TB (BCPTB) (n = 140) patients. Lowenstein-Jensen (LJ) culture, geneXpert and acid fast staining were performed on sputum specimen collected from both CDPTB and BCPTB patients. Spoligotyping, whole genome sequencing (WGS) and phenotypic drug resistance testing (DST) were done for a subset of LJ grown MTBC isolates. Data was analyzed by STATA version 17 software and a p-value <0.05 were considered statistically significant.ResultsThe proportion of lineage 3 was larger among CDPTB patients (31%, 13/42) compared to BCPTB patients (15%, 11/74) (p-value <0.05). A higher proportion of MTBC isolates from CDPTB 16.6% (3/18) were phenotypically resistant to one or more anti-TB drugs than BCPTB 12% (4/33) (p-value >0.05). A single lineage 3 strain resistant to all the primary anti-TB drugs was detected in one CDPTB by both DST methods.ConclusionThe observed differences in the genotypes of MTBC isolates between CDPTB and BCPTB patients may be attributed to challenges in the identification of CDPTB that requires further investigation on sequenced genome of the MTBC strains for better understanding and recommendation based on the current finding. There was also primary drug resistant TB among culture positive CDPTB patients which would be otherwise missed by current national protocols. |
| format | Article |
| id | doaj-art-18fbb1fa9f1d4104a7c883d9bcf69629 |
| institution | DOAJ |
| issn | 2296-2565 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj-art-18fbb1fa9f1d4104a7c883d9bcf696292025-08-20T02:51:14ZengFrontiers Media S.A.Frontiers in Public Health2296-25652024-12-011210.3389/fpubh.2024.14206851420685Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patientsAlem Alemayehu0Alem Alemayehu1Alem Alemayehu2Liya Wassie3Dawit Hailu Alemayehu4Bethlehem Adnew5Sebsib Neway6Dessalegn Abeje Tefera7Sosina Ayalew8Elena Hailu9Samuel Ayele10Berhanu Seyoum11Kidist Bobosha12Markos Abebe13Abraham Aseffa14Beyene Petros15Rawleigh Howe16Department of Microbial, Cellular and Molecular Biology, College of Natural and Computational Sciences, Addis Ababa University, Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaSchool of Medial Laboratory Science, College of Health and Medical Sciences, Haramaya University, Dire Dawa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaDepartment of Microbial, Cellular and Molecular Biology, College of Natural and Computational Sciences, Addis Ababa University, Addis Ababa, EthiopiaArmauer Hansen Research Institute (AHRI), Addis Ababa, EthiopiaBackgroundClinically diagnosed pulmonary tuberculosis (TB) (CDPTB) patients account for a huge proportion of TB. However, little is known about the genetic diversity and drug resistance profile of Mycobacterium tuberculosis Complex (MTBC) strains in this group of patients.MethodUnmatched case–control study was conducted among 313 PTB patients to compare the genetic diversity of MTBC and their drug resistance profiles among CDPTB (n = 173) and bacteriologically confirmed pulmonary TB (BCPTB) (n = 140) patients. Lowenstein-Jensen (LJ) culture, geneXpert and acid fast staining were performed on sputum specimen collected from both CDPTB and BCPTB patients. Spoligotyping, whole genome sequencing (WGS) and phenotypic drug resistance testing (DST) were done for a subset of LJ grown MTBC isolates. Data was analyzed by STATA version 17 software and a p-value <0.05 were considered statistically significant.ResultsThe proportion of lineage 3 was larger among CDPTB patients (31%, 13/42) compared to BCPTB patients (15%, 11/74) (p-value <0.05). A higher proportion of MTBC isolates from CDPTB 16.6% (3/18) were phenotypically resistant to one or more anti-TB drugs than BCPTB 12% (4/33) (p-value >0.05). A single lineage 3 strain resistant to all the primary anti-TB drugs was detected in one CDPTB by both DST methods.ConclusionThe observed differences in the genotypes of MTBC isolates between CDPTB and BCPTB patients may be attributed to challenges in the identification of CDPTB that requires further investigation on sequenced genome of the MTBC strains for better understanding and recommendation based on the current finding. There was also primary drug resistant TB among culture positive CDPTB patients which would be otherwise missed by current national protocols.https://www.frontiersin.org/articles/10.3389/fpubh.2024.1420685/fullBCPTBCDPTBEthiopiageneXpertsmear-microscopyMTBC |
| spellingShingle | Alem Alemayehu Alem Alemayehu Alem Alemayehu Liya Wassie Dawit Hailu Alemayehu Bethlehem Adnew Sebsib Neway Dessalegn Abeje Tefera Sosina Ayalew Elena Hailu Samuel Ayele Berhanu Seyoum Kidist Bobosha Markos Abebe Abraham Aseffa Beyene Petros Rawleigh Howe Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients Frontiers in Public Health BCPTB CDPTB Ethiopia geneXpert smear-microscopy MTBC |
| title | Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients |
| title_full | Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients |
| title_fullStr | Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients |
| title_full_unstemmed | Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients |
| title_short | Genotypes and drug resistance pattern of Mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients |
| title_sort | genotypes and drug resistance pattern of mycobacterium tuberculosis complex among clinically diagnosed pulmonary tuberculosis patients |
| topic | BCPTB CDPTB Ethiopia geneXpert smear-microscopy MTBC |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2024.1420685/full |
| work_keys_str_mv | AT alemalemayehu genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT alemalemayehu genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT alemalemayehu genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT liyawassie genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT dawithailualemayehu genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT bethlehemadnew genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT sebsibneway genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT dessalegnabejetefera genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT sosinaayalew genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT elenahailu genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT samuelayele genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT berhanuseyoum genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT kidistbobosha genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT markosabebe genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT abrahamaseffa genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT beyenepetros genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients AT rawleighhowe genotypesanddrugresistancepatternofmycobacteriumtuberculosiscomplexamongclinicallydiagnosedpulmonarytuberculosispatients |