MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
Gastric cancer (GC) accounts for a main cause of cancer-related deaths. This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC. Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA databa...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2021/2894117 |
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| _version_ | 1849309221667995648 |
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| author | Jianjie Li Xiaoyue Xu Chunhui Liu Xiaoxue Xi Yang Wang Xiaotang Wu Hua Li |
| author_facet | Jianjie Li Xiaoyue Xu Chunhui Liu Xiaoxue Xi Yang Wang Xiaotang Wu Hua Li |
| author_sort | Jianjie Li |
| collection | DOAJ |
| description | Gastric cancer (GC) accounts for a main cause of cancer-related deaths. This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC. Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA database were analyzed. MiR-490-5p and DTL mRNA expression levels in GC were evaluated with qRT-PCR. Cell viability was confirmed with CCK-8 method. Cell cycle distribution and apoptosis were analyzed with flow cytometry. Cell migratory and invasive potential was proved with Transwell assay. The targeted relationship between DTL and miR-490-5p was analyzed with dual-luciferase assay. The results indicated a decreased miR-490-5p level in GC cells. MiR-490-5p upregulation hampered proliferation, migration, invasion and promote cell apoptosis. DTL was the target of and inversely associated with miR-490-5p, and it could remarkably induce the carcinogenesis of GC. MiR-490-5p mediated GC cell progression by DTL repression. In conclusion, miR-490-5p and DTL may be valuable in diagnosis and treatment for GC. |
| format | Article |
| id | doaj-art-18f0ce9671fc4bdc87f8b772a19edb82 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-18f0ce9671fc4bdc87f8b772a19edb822025-08-20T03:54:14ZengWileyGastroenterology Research and Practice1687-61211687-630X2021-01-01202110.1155/2021/28941172894117MiR-490-5p Restrains Progression of Gastric cancer through DTL RepressionJianjie Li0Xiaoyue Xu1Chunhui Liu2Xiaoxue Xi3Yang Wang4Xiaotang Wu5Hua Li6Department of Gastrointestinal Surgery, Tangshan Central Hospital, Tangshan, 06300 Hebei, ChinaDepartment of Gastrointestinal Surgery, Tangshan Gongren Hospital, Tangshan, 063000 Hebei, ChinaDepartment of General Surgery, North China University of Science and Technology Affiliated Hospital, 063000 Hebei, ChinaDepartment of Gastrointestinal Surgery, Tangshan Central Hospital, Tangshan, 06300 Hebei, ChinaDepartment of Gastrointestinal Surgery, Tangshan Central Hospital, Tangshan, 06300 Hebei, ChinaShanghai Engineering Research Center of Pharmaceutical Translation, 200231, ChinaDepartment of Gastrointestinal Surgery, Tangshan Central Hospital, Tangshan, 06300 Hebei, ChinaGastric cancer (GC) accounts for a main cause of cancer-related deaths. This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC. Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA database were analyzed. MiR-490-5p and DTL mRNA expression levels in GC were evaluated with qRT-PCR. Cell viability was confirmed with CCK-8 method. Cell cycle distribution and apoptosis were analyzed with flow cytometry. Cell migratory and invasive potential was proved with Transwell assay. The targeted relationship between DTL and miR-490-5p was analyzed with dual-luciferase assay. The results indicated a decreased miR-490-5p level in GC cells. MiR-490-5p upregulation hampered proliferation, migration, invasion and promote cell apoptosis. DTL was the target of and inversely associated with miR-490-5p, and it could remarkably induce the carcinogenesis of GC. MiR-490-5p mediated GC cell progression by DTL repression. In conclusion, miR-490-5p and DTL may be valuable in diagnosis and treatment for GC.http://dx.doi.org/10.1155/2021/2894117 |
| spellingShingle | Jianjie Li Xiaoyue Xu Chunhui Liu Xiaoxue Xi Yang Wang Xiaotang Wu Hua Li MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression Gastroenterology Research and Practice |
| title | MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression |
| title_full | MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression |
| title_fullStr | MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression |
| title_full_unstemmed | MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression |
| title_short | MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression |
| title_sort | mir 490 5p restrains progression of gastric cancer through dtl repression |
| url | http://dx.doi.org/10.1155/2021/2894117 |
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