Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony

The peroxidase isozymes of Hangzhou white peony were isolated by polyacrylamide gel electrophoresis. Up to 2-5 active bands of peroxidase isozyme were observed in 13 lines after active dyeing, and the rate of relative migration was located in the range from 0.567 to 0.685. Based on the differences i...

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Main Authors: SONG Qin, HU Jia-shu, TONG Fu-dan, HU Qiao-ping
Format: Article
Language:English
Published: Zhejiang University Press 2005-07-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2005.04.0454
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author SONG Qin
HU Jia-shu
TONG Fu-dan
HU Qiao-ping
author_facet SONG Qin
HU Jia-shu
TONG Fu-dan
HU Qiao-ping
author_sort SONG Qin
collection DOAJ
description The peroxidase isozymes of Hangzhou white peony were isolated by polyacrylamide gel electrophoresis. Up to 2-5 active bands of peroxidase isozyme were observed in 13 lines after active dyeing, and the rate of relative migration was located in the range from 0.567 to 0.685. Based on the differences in number and location of bands the individuals were divided into 3 groups. The results of HPLC showed that paeoniflorin was the primary component in Hangzhou white peony, and only a little albiflorin with no oxypaeoniflorin was observed. The paeoniflorin content of Hangzhou white peony as detected after different processing was different, although the HPLC chromatograms of paeoniflorin were similar.
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issn 1008-9209
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language English
publishDate 2005-07-01
publisher Zhejiang University Press
record_format Article
series 浙江大学学报. 农业与生命科学版
spelling doaj-art-18eb48099fbf403bb3e01e400e09aede2025-08-20T03:16:04ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552005-07-013145445810.3785/1008-9209.2005.04.045410089209Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peonySONG QinHU Jia-shuTONG Fu-danHU Qiao-pingThe peroxidase isozymes of Hangzhou white peony were isolated by polyacrylamide gel electrophoresis. Up to 2-5 active bands of peroxidase isozyme were observed in 13 lines after active dyeing, and the rate of relative migration was located in the range from 0.567 to 0.685. Based on the differences in number and location of bands the individuals were divided into 3 groups. The results of HPLC showed that paeoniflorin was the primary component in Hangzhou white peony, and only a little albiflorin with no oxypaeoniflorin was observed. The paeoniflorin content of Hangzhou white peony as detected after different processing was different, although the HPLC chromatograms of paeoniflorin were similar.https://www.academax.com/doi/10.3785/1008-9209.2005.04.0454Hangzhou white peonyperoxidaseisozymepaeoniflorinHPLC
spellingShingle SONG Qin
HU Jia-shu
TONG Fu-dan
HU Qiao-ping
Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony
浙江大学学报. 农业与生命科学版
Hangzhou white peony
peroxidase
isozyme
paeoniflorin
HPLC
title Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony
title_full Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony
title_fullStr Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony
title_full_unstemmed Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony
title_short Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony
title_sort analysis of peroxidase isozymes and hplc chromatographic study of paeoniflorin in hangzhou white peony
topic Hangzhou white peony
peroxidase
isozyme
paeoniflorin
HPLC
url https://www.academax.com/doi/10.3785/1008-9209.2005.04.0454
work_keys_str_mv AT songqin analysisofperoxidaseisozymesandhplcchromatographicstudyofpaeoniflorininhangzhouwhitepeony
AT hujiashu analysisofperoxidaseisozymesandhplcchromatographicstudyofpaeoniflorininhangzhouwhitepeony
AT tongfudan analysisofperoxidaseisozymesandhplcchromatographicstudyofpaeoniflorininhangzhouwhitepeony
AT huqiaoping analysisofperoxidaseisozymesandhplcchromatographicstudyofpaeoniflorininhangzhouwhitepeony