Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system

Maintaining low nitrate concentrations in aquaponic systems is crucial for improving water quality and maximizing the growth efficiency of fish and vegetables. Downflow hanging sponge (DHS) and upflow sludge blanket (USB) reactors have shown potential for wastewater treatment, but their use in aquap...

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Main Authors: Limin Teng, Takahiro Watari, Mami Nagai, Nur Adlin, Penpicha Satanwat, Masashi Hatamoto, Takashi Yamaguchi
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Water Science and Engineering
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Online Access:http://www.sciencedirect.com/science/article/pii/S1674237025000298
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author Limin Teng
Takahiro Watari
Mami Nagai
Nur Adlin
Penpicha Satanwat
Masashi Hatamoto
Takashi Yamaguchi
author_facet Limin Teng
Takahiro Watari
Mami Nagai
Nur Adlin
Penpicha Satanwat
Masashi Hatamoto
Takashi Yamaguchi
author_sort Limin Teng
collection DOAJ
description Maintaining low nitrate concentrations in aquaponic systems is crucial for improving water quality and maximizing the growth efficiency of fish and vegetables. Downflow hanging sponge (DHS) and upflow sludge blanket (USB) reactors have shown potential for wastewater treatment, but their use in aquaponic systems is relatively underexplored, particularly for overall performance and efficiency. In this study, a DHS reactor was coupled with a denitrifying USB reactor in an aquaponic system comprising Nile tilapia (Oreochromis niloticus) and kale (Brassica oleracea L. var. acephala DC). The USB reactor achieved a nitrate removal rate of 80.8% ± 20.5%. The specific growth rate of tilapia was 6.11% per day from day 16 to day 30. On day 45, kale growth achieved stem lengths of (4.1 ± 1.2) cm, root lengths of (12.2 ± 6.0) cm, and leaf counts of (6.3 ± 2.0) leaves per plant. Changes in the microbial communities within the reactors positively contributed to denitrification, resulting in a nitrogen utilization efficiency of 88.3%. The DHS–USB aquaponic system effectively maintained optimal water quality and stable parameters (pH, dissolved oxygen, and temperature). It regulated ammonia levels well and achieved 80.8% ± 20.5% removal rates for nitrite and nitrate after day 10. Microbial analysis highlighted significant shifts in the microbial communities within the DHS and USB reactors, underscoring their critical roles in nitrification and denitrification. Therefore, the DHS–USB aquatic system has the potential to improve agricultural production efficiency and promote sustainable development.
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spelling doaj-art-18e04b56c9a8460a8e68ce760b7fc02a2025-08-23T04:47:52ZengElsevierWater Science and Engineering1674-23702025-09-0118334535310.1016/j.wse.2025.04.004Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic systemLimin Teng0Takahiro Watari1Mami Nagai2Nur Adlin3Penpicha Satanwat4Masashi Hatamoto5Takashi Yamaguchi6Department of Energy and Environment Science, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, JapanDepartment of Civil and Environmental Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan; Corresponding author.Department of Civil and Environmental Engineering, National Institute of Technology, Oita College, Oita 870-0152, Japan; Department of Science of Technology Innovation, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, JapanDepartment of Science of Technology Innovation, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, JapanDepartment of Civil Engineering, Faculty of Engineering, Thammasat School of Engineering, Thammasat University, Pathum Thani 12120, ThailandDepartment of Civil and Environmental Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, JapanDepartment of Civil and Environmental Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan; Department of Science of Technology Innovation, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, JapanMaintaining low nitrate concentrations in aquaponic systems is crucial for improving water quality and maximizing the growth efficiency of fish and vegetables. Downflow hanging sponge (DHS) and upflow sludge blanket (USB) reactors have shown potential for wastewater treatment, but their use in aquaponic systems is relatively underexplored, particularly for overall performance and efficiency. In this study, a DHS reactor was coupled with a denitrifying USB reactor in an aquaponic system comprising Nile tilapia (Oreochromis niloticus) and kale (Brassica oleracea L. var. acephala DC). The USB reactor achieved a nitrate removal rate of 80.8% ± 20.5%. The specific growth rate of tilapia was 6.11% per day from day 16 to day 30. On day 45, kale growth achieved stem lengths of (4.1 ± 1.2) cm, root lengths of (12.2 ± 6.0) cm, and leaf counts of (6.3 ± 2.0) leaves per plant. Changes in the microbial communities within the reactors positively contributed to denitrification, resulting in a nitrogen utilization efficiency of 88.3%. The DHS–USB aquaponic system effectively maintained optimal water quality and stable parameters (pH, dissolved oxygen, and temperature). It regulated ammonia levels well and achieved 80.8% ± 20.5% removal rates for nitrite and nitrate after day 10. Microbial analysis highlighted significant shifts in the microbial communities within the DHS and USB reactors, underscoring their critical roles in nitrification and denitrification. Therefore, the DHS–USB aquatic system has the potential to improve agricultural production efficiency and promote sustainable development.http://www.sciencedirect.com/science/article/pii/S1674237025000298AquaponicsDenitrificationDHS reactorMicrobial communityNitrogen removal
spellingShingle Limin Teng
Takahiro Watari
Mami Nagai
Nur Adlin
Penpicha Satanwat
Masashi Hatamoto
Takashi Yamaguchi
Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system
Water Science and Engineering
Aquaponics
Denitrification
DHS reactor
Microbial community
Nitrogen removal
title Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system
title_full Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system
title_fullStr Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system
title_full_unstemmed Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system
title_short Performance evaluation of downflow hanging sponge–upflow sludge blanket system for Oreochromis niloticus–Brassica oleracea aquaponic system
title_sort performance evaluation of downflow hanging sponge upflow sludge blanket system for oreochromis niloticus brassica oleracea aquaponic system
topic Aquaponics
Denitrification
DHS reactor
Microbial community
Nitrogen removal
url http://www.sciencedirect.com/science/article/pii/S1674237025000298
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