First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes
Objective: VanD-type vancomycin resistance is rarely reported in Enterococcus faecalis. This study aimed to characterise five VanD-type vancomycin-resistant E. faecalis strains isolated over 8 years from three hospitals in a local city in Japan, with a focus on resistance mechanisms, genetic backgro...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716525001791 |
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| author | Kensuke Mimura Yusuke Hashimoto Jun Kurushima Hidetada Hirakawa Takahiro Nomura Koichi Tanimoto Tetsuro Muratani Daisuke Todokoro Hideo Akiyama Haruyoshi Tomita |
| author_facet | Kensuke Mimura Yusuke Hashimoto Jun Kurushima Hidetada Hirakawa Takahiro Nomura Koichi Tanimoto Tetsuro Muratani Daisuke Todokoro Hideo Akiyama Haruyoshi Tomita |
| author_sort | Kensuke Mimura |
| collection | DOAJ |
| description | Objective: VanD-type vancomycin resistance is rarely reported in Enterococcus faecalis. This study aimed to characterise five VanD-type vancomycin-resistant E. faecalis strains isolated over 8 years from three hospitals in a local city in Japan, with a focus on resistance mechanisms, genetic background, and phylogenetic classification. Methods: Five E. faecalis strains (SVR2085, SVR2281, SVR2330, SVR2331, and SVR2332) were analysed using antimicrobial susceptibility testing, plasmid profiling, and whole-genome sequencing. Genomic islands (GIs) containing vanD gene clusters were characterised. Core GI gene and core genome phylogenies were compared, and vanD homologues were classified using public database sequences. Results: All strains exhibited high-level resistance to vancomycin (MIC >1024–64 mg/L), multiple drug resistance, and carried pheromone-responsive plasmids encoding bacteriocin 41 as a colonisation factor. Four strains shared nearly identical GIs (126–185 kbp), while SVR2330 had a structurally distinct GI. Phylogenetic analysis showed that the four similar strains formed a single cluster, suggesting a common ancestor, whereas SVR2330 was divergent. Comparative analysis of 37 vanD homologues revealed high genetic diversity, allowing classification into three subgroups – vanD(I), vanD(II), and vanD(III). The vanD of SVR2330 was assigned as vanD(III)-4, and the others as vanD(II)-6, -11, -15, and -17. Conclusions: This study proposes a new classification scheme for diverse vanD genes and provides the first evidence of the regional spread and long-term persistence of VanD-type vancomycin-resistant E. faecalis in Japan. |
| format | Article |
| id | doaj-art-18cfb46d8eb24a028d1349424d27901d |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-18cfb46d8eb24a028d1349424d27901d2025-08-23T04:48:23ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-09-014439841010.1016/j.jgar.2025.07.021First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypesKensuke Mimura0Yusuke Hashimoto1Jun Kurushima2Hidetada Hirakawa3Takahiro Nomura4Koichi Tanimoto5Tetsuro Muratani6Daisuke Todokoro7Hideo Akiyama8Haruyoshi Tomita9Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanLaboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanFaculty of medical technology, Gunma University of Health and Welfare, 191-1 Kawamagari-cho, Maebashi City, Gunma, JapanHibiki AMR Laboratory, 1-12-16 Mitsusadadai, Yahatanishi-ku, Kitakyushu, Fukuoka, Japan; Aisihinkai Kokura Itozu Hospital, 5-10-31 Shimoitozu, Kokurakita-ku, Kitakyushu, Fukuoka, JapanDepartment of Ophthalmology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanDepartment of Ophthalmology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, Japan; Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, Japan; Corresponding author. Mailing address: Department of Bacteriology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, Japan 371-8511.Objective: VanD-type vancomycin resistance is rarely reported in Enterococcus faecalis. This study aimed to characterise five VanD-type vancomycin-resistant E. faecalis strains isolated over 8 years from three hospitals in a local city in Japan, with a focus on resistance mechanisms, genetic background, and phylogenetic classification. Methods: Five E. faecalis strains (SVR2085, SVR2281, SVR2330, SVR2331, and SVR2332) were analysed using antimicrobial susceptibility testing, plasmid profiling, and whole-genome sequencing. Genomic islands (GIs) containing vanD gene clusters were characterised. Core GI gene and core genome phylogenies were compared, and vanD homologues were classified using public database sequences. Results: All strains exhibited high-level resistance to vancomycin (MIC >1024–64 mg/L), multiple drug resistance, and carried pheromone-responsive plasmids encoding bacteriocin 41 as a colonisation factor. Four strains shared nearly identical GIs (126–185 kbp), while SVR2330 had a structurally distinct GI. Phylogenetic analysis showed that the four similar strains formed a single cluster, suggesting a common ancestor, whereas SVR2330 was divergent. Comparative analysis of 37 vanD homologues revealed high genetic diversity, allowing classification into three subgroups – vanD(I), vanD(II), and vanD(III). The vanD of SVR2330 was assigned as vanD(III)-4, and the others as vanD(II)-6, -11, -15, and -17. Conclusions: This study proposes a new classification scheme for diverse vanD genes and provides the first evidence of the regional spread and long-term persistence of VanD-type vancomycin-resistant E. faecalis in Japan.http://www.sciencedirect.com/science/article/pii/S2213716525001791VancomycinVREVanD typeEnterococcus faecalisGenomic islandNew classification |
| spellingShingle | Kensuke Mimura Yusuke Hashimoto Jun Kurushima Hidetada Hirakawa Takahiro Nomura Koichi Tanimoto Tetsuro Muratani Daisuke Todokoro Hideo Akiyama Haruyoshi Tomita First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes Journal of Global Antimicrobial Resistance Vancomycin VRE VanD type Enterococcus faecalis Genomic island New classification |
| title | First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes |
| title_full | First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes |
| title_fullStr | First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes |
| title_full_unstemmed | First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes |
| title_short | First report of regional spreading and long-term inhabitation of VanD-type vancomycin-resistant Enterococcus faecalis clinical strains: A proposal of new classification of vanD gene subtypes |
| title_sort | first report of regional spreading and long term inhabitation of vand type vancomycin resistant enterococcus faecalis clinical strains a proposal of new classification of vand gene subtypes |
| topic | Vancomycin VRE VanD type Enterococcus faecalis Genomic island New classification |
| url | http://www.sciencedirect.com/science/article/pii/S2213716525001791 |
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