Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan
Orthohantavirus infection is a zoonotic disease transmitted to humans through contact with infected rodents. In Eurasia, Old World Orthohantaviruses can cause haemorrhagic fever with renal syndrome (HFRS), while in the Americas, New World Orthohantaviruses are responsible for hantavirus cardiopulmon...
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| Format: | Article |
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MDPI AG
2025-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/7/925 |
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| author | Nur Tukhanova Anna Shin Abhishek Bakuli Lyazzat Yeraliyeva Nurbek Maikanov Guenter Froeschl Zauresh Zhumadilova Gulnara Tokmurziyeva Edith Wagner Sandra Essbauer Lukas Peintner |
| author_facet | Nur Tukhanova Anna Shin Abhishek Bakuli Lyazzat Yeraliyeva Nurbek Maikanov Guenter Froeschl Zauresh Zhumadilova Gulnara Tokmurziyeva Edith Wagner Sandra Essbauer Lukas Peintner |
| author_sort | Nur Tukhanova |
| collection | DOAJ |
| description | Orthohantavirus infection is a zoonotic disease transmitted to humans through contact with infected rodents. In Eurasia, Old World Orthohantaviruses can cause haemorrhagic fever with renal syndrome (HFRS), while in the Americas, New World Orthohantaviruses are responsible for hantavirus cardiopulmonary syndrome (HCPS). In Kazakhstan, the first recorded cases of HFRS appeared in the West Kazakhstan region in 2000, which has since then been established as an endemic area due to the presence of stable rodent reservoirs and recurring human infections. Routine diagnosis of HFRS in this region relies primarily on immunoassays. To enhance diagnostic precision, we aimed to implement both serological and molecular methods on samples from suspected HFRS cases in the endemic West Kazakhstan region and non-endemic Almaty City. A total of 139 paired serum, saliva, and urine samples were analysed using IgM/IgG ELISA, immunoblot assays, and qPCR. Our findings confirm that suspected HFRS cases in West Kazakhstan are associated with the Puumala virus serotype. |
| format | Article |
| id | doaj-art-18cc960bab9d40bf8bb48bc3e66a46b5 |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-18cc960bab9d40bf8bb48bc3e66a46b52025-08-20T02:47:18ZengMDPI AGViruses1999-49152025-06-0117792510.3390/v17070925Characterisation of Orthohantavirus Serotypes in Human Infections in KazakhstanNur Tukhanova0Anna Shin1Abhishek Bakuli2Lyazzat Yeraliyeva3Nurbek Maikanov4Guenter Froeschl5Zauresh Zhumadilova6Gulnara Tokmurziyeva7Edith Wagner8Sandra Essbauer9Lukas Peintner10M. Aikimbayev’s National Scientific Center of Especially Dangerous Infections, 050054 Almaty, KazakhstanDepartment of General Medical Practice, Al-Farabi Kazakh National University, 050040 Almaty, KazakhstanDivision of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, 80802 Munich, GermanyNational Scientific Center of Phthisiopulmonology, 050010 Almaty, KazakhstanOral Antiplague Station, 090001 Oral, KazakhstanDivision of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, 80802 Munich, GermanyM. Aikimbayev’s National Scientific Center of Especially Dangerous Infections, 050054 Almaty, KazakhstanM. Aikimbayev’s National Scientific Center of Especially Dangerous Infections, 050054 Almaty, KazakhstanInstitute of Medical Microbiology, Jena University Hospital, 07747 Jena, GermanyDepartment of Virology and Intracellular Agents, Bundeswehr Institute of Microbiology, 80937 Munich, GermanyInstitute of Molecular Medicine and Cell Research, University of Freiburg, 79104 Freiburg im Breisgau, GermanyOrthohantavirus infection is a zoonotic disease transmitted to humans through contact with infected rodents. In Eurasia, Old World Orthohantaviruses can cause haemorrhagic fever with renal syndrome (HFRS), while in the Americas, New World Orthohantaviruses are responsible for hantavirus cardiopulmonary syndrome (HCPS). In Kazakhstan, the first recorded cases of HFRS appeared in the West Kazakhstan region in 2000, which has since then been established as an endemic area due to the presence of stable rodent reservoirs and recurring human infections. Routine diagnosis of HFRS in this region relies primarily on immunoassays. To enhance diagnostic precision, we aimed to implement both serological and molecular methods on samples from suspected HFRS cases in the endemic West Kazakhstan region and non-endemic Almaty City. A total of 139 paired serum, saliva, and urine samples were analysed using IgM/IgG ELISA, immunoblot assays, and qPCR. Our findings confirm that suspected HFRS cases in West Kazakhstan are associated with the Puumala virus serotype.https://www.mdpi.com/1999-4915/17/7/925Kazakhstanorthohantavirusserologyhuman infections |
| spellingShingle | Nur Tukhanova Anna Shin Abhishek Bakuli Lyazzat Yeraliyeva Nurbek Maikanov Guenter Froeschl Zauresh Zhumadilova Gulnara Tokmurziyeva Edith Wagner Sandra Essbauer Lukas Peintner Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan Viruses Kazakhstan orthohantavirus serology human infections |
| title | Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan |
| title_full | Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan |
| title_fullStr | Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan |
| title_full_unstemmed | Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan |
| title_short | Characterisation of Orthohantavirus Serotypes in Human Infections in Kazakhstan |
| title_sort | characterisation of orthohantavirus serotypes in human infections in kazakhstan |
| topic | Kazakhstan orthohantavirus serology human infections |
| url | https://www.mdpi.com/1999-4915/17/7/925 |
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