Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG
Tissue-resident memory T cells (TRM) are different from effector memory T cells (TEM) and central memory T cells (TCM) and contribute to the protective immunity against local challenges. Currently, we found that CD4+ and CD8+ TRM cells in the nasal mucosa, trachea, lungs, and lavage fluids were hete...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/6660379 |
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| author | Qiongli Wu Shuangpeng Kang Jun Huang Shunqiao Wan Binyan Yang Changyou Wu |
| author_facet | Qiongli Wu Shuangpeng Kang Jun Huang Shunqiao Wan Binyan Yang Changyou Wu |
| author_sort | Qiongli Wu |
| collection | DOAJ |
| description | Tissue-resident memory T cells (TRM) are different from effector memory T cells (TEM) and central memory T cells (TCM) and contribute to the protective immunity against local challenges. Currently, we found that CD4+ and CD8+ TRM cells in the nasal mucosa, trachea, lungs, and lavage fluids were heterogeneous on the expression of CD69 and CD103 as well as the production of cytokines including IFN-γ, IL-2, and TNF-α. After intranasal vaccination of mice with BCG, respiratory tissues expressed higher levels of the chemokine CXCL16 and TRM cells expressed CXCR6 to CXCL16. In addition, antigen-specific CD4+ and CD8+ TRM cells expressed cytokines following the stimulation with BCG and persisted in the nasal mucosa, trachea, and lungs for more than a hundred days. At the same time, mice were infected intranasally with live BCG and the results showed that vaccinated mice cleared up live BCG faster than nonvaccinated mice in the respiratory system. Taken together, our data demonstrated that intranasal vaccination of mice with BCG could induce antigen-specific CD4+ and CD8+ TRM cells in the respiratory system and have the ability to provide protection against pulmonary reinfection. |
| format | Article |
| id | doaj-art-18cb87156bd244588294729fdffb0183 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-18cb87156bd244588294729fdffb01832025-02-03T06:05:36ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/66603796660379Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCGQiongli Wu0Shuangpeng Kang1Jun Huang2Shunqiao Wan3Binyan Yang4Changyou Wu5Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaInstitute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaKey Laboratory of Immunology, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou, ChinaInstitute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaInstitute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaInstitute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaTissue-resident memory T cells (TRM) are different from effector memory T cells (TEM) and central memory T cells (TCM) and contribute to the protective immunity against local challenges. Currently, we found that CD4+ and CD8+ TRM cells in the nasal mucosa, trachea, lungs, and lavage fluids were heterogeneous on the expression of CD69 and CD103 as well as the production of cytokines including IFN-γ, IL-2, and TNF-α. After intranasal vaccination of mice with BCG, respiratory tissues expressed higher levels of the chemokine CXCL16 and TRM cells expressed CXCR6 to CXCL16. In addition, antigen-specific CD4+ and CD8+ TRM cells expressed cytokines following the stimulation with BCG and persisted in the nasal mucosa, trachea, and lungs for more than a hundred days. At the same time, mice were infected intranasally with live BCG and the results showed that vaccinated mice cleared up live BCG faster than nonvaccinated mice in the respiratory system. Taken together, our data demonstrated that intranasal vaccination of mice with BCG could induce antigen-specific CD4+ and CD8+ TRM cells in the respiratory system and have the ability to provide protection against pulmonary reinfection.http://dx.doi.org/10.1155/2021/6660379 |
| spellingShingle | Qiongli Wu Shuangpeng Kang Jun Huang Shunqiao Wan Binyan Yang Changyou Wu Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG Journal of Immunology Research |
| title | Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG |
| title_full | Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG |
| title_fullStr | Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG |
| title_full_unstemmed | Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG |
| title_short | Antigen-Specific Tissue-Resident Memory T Cells in the Respiratory System Were Generated following Intranasal Vaccination of Mice with BCG |
| title_sort | antigen specific tissue resident memory t cells in the respiratory system were generated following intranasal vaccination of mice with bcg |
| url | http://dx.doi.org/10.1155/2021/6660379 |
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