Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces
Actinomycetes are a vital group of Gram-positive bacteria known for producing a wide range of bioactive secondary metabolites, including important antibiotics. These microorganisms play an essential role in the degradation of organic matter and nutrient cycling, contributing significantly to soil he...
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Ferdowsi University of Mashhad
2025-03-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
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| Online Access: | https://ijvst.um.ac.ir/article_46507_452dbd9427fd685b02d7552d20d16c99.pdf |
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| author | Talieh Mohammadi Fazel Pourahmad Khalil Saleki |
| author_facet | Talieh Mohammadi Fazel Pourahmad Khalil Saleki |
| author_sort | Talieh Mohammadi |
| collection | DOAJ |
| description | Actinomycetes are a vital group of Gram-positive bacteria known for producing a wide range of bioactive secondary metabolites, including important antibiotics. These microorganisms play an essential role in the degradation of organic matter and nutrient cycling, contributing significantly to soil health and fertility. Their capacity to synthesize diverse compounds and the presence of key biosynthetic pathways involving polyketide synthases and non-ribosomal peptide synthetases highlights their potential in antibiotic discovery, particularly against antibiotic-resistant pathogens. This study aimed to isolate and characterize Actinomycetes from fresh sheep feces collected in Ilam Province, Iran, focusing on their antibacterial activity and biosynthetic potential. A total of 86 actinomycete isolates were obtained from fecal samples collected from sheep in 2021. Morphological characterization confirmed all isolates as Gram-positive and filamentous. Molecular identification through PCR amplification of the 16S rRNA gene yielded a product of approximately 640 base pairs for all isolates. Antibacterial screening revealed that 17 isolates exhibited activity against various pathogens, with the highest efficacy observed against Bacillus cereus (62.1%). Molecular analysis also indicated the presence of biosynthetic gene clusters, with 31 isolates (36.05%) bearing non-ribosomal peptide synthetase (NRPS) gene, 15 isolates (17.44%) containing polyketide synthase I (PKS-I), and 16 isolates (18.6%) with polyketide synthase II (PKS-II) genes. This study highlights the significant antibacterial properties and biosynthetic capabilities of actinomycetes from sheep feces, suggesting their potential use in therapeutic, agriculture, and biotechnological applications. |
| format | Article |
| id | doaj-art-18c30547cded43a19c0657be89fc0a97 |
| institution | DOAJ |
| issn | 2008-465X 2423-6306 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj-art-18c30547cded43a19c0657be89fc0a972025-08-20T03:18:33ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062025-03-011719410110.22067/ijvst.2025.91803.146646507Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep FecesTalieh Mohammadi0Fazel Pourahmad1Khalil Saleki2Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, IranDepartment of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, IranDepartment of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, IranActinomycetes are a vital group of Gram-positive bacteria known for producing a wide range of bioactive secondary metabolites, including important antibiotics. These microorganisms play an essential role in the degradation of organic matter and nutrient cycling, contributing significantly to soil health and fertility. Their capacity to synthesize diverse compounds and the presence of key biosynthetic pathways involving polyketide synthases and non-ribosomal peptide synthetases highlights their potential in antibiotic discovery, particularly against antibiotic-resistant pathogens. This study aimed to isolate and characterize Actinomycetes from fresh sheep feces collected in Ilam Province, Iran, focusing on their antibacterial activity and biosynthetic potential. A total of 86 actinomycete isolates were obtained from fecal samples collected from sheep in 2021. Morphological characterization confirmed all isolates as Gram-positive and filamentous. Molecular identification through PCR amplification of the 16S rRNA gene yielded a product of approximately 640 base pairs for all isolates. Antibacterial screening revealed that 17 isolates exhibited activity against various pathogens, with the highest efficacy observed against Bacillus cereus (62.1%). Molecular analysis also indicated the presence of biosynthetic gene clusters, with 31 isolates (36.05%) bearing non-ribosomal peptide synthetase (NRPS) gene, 15 isolates (17.44%) containing polyketide synthase I (PKS-I), and 16 isolates (18.6%) with polyketide synthase II (PKS-II) genes. This study highlights the significant antibacterial properties and biosynthetic capabilities of actinomycetes from sheep feces, suggesting their potential use in therapeutic, agriculture, and biotechnological applications.https://ijvst.um.ac.ir/article_46507_452dbd9427fd685b02d7552d20d16c99.pdfactinomycetespolyketide synthasenon-ribosomal peptide synthetaseantibacterial activitysheep feces |
| spellingShingle | Talieh Mohammadi Fazel Pourahmad Khalil Saleki Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces The Iranian Journal of Veterinary Science and Technology actinomycetes polyketide synthase non-ribosomal peptide synthetase antibacterial activity sheep feces |
| title | Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces |
| title_full | Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces |
| title_fullStr | Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces |
| title_full_unstemmed | Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces |
| title_short | Isolation, Characterization, and Antibacterial Activity of Actinomycetes from Sheep Feces |
| title_sort | isolation characterization and antibacterial activity of actinomycetes from sheep feces |
| topic | actinomycetes polyketide synthase non-ribosomal peptide synthetase antibacterial activity sheep feces |
| url | https://ijvst.um.ac.ir/article_46507_452dbd9427fd685b02d7552d20d16c99.pdf |
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