Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis
Objective: Hyaluronic acid (HA) is a naturally occurring, biocompatible polysaccharide with unique viscoelastic and hygroscopic properties. Its role as a natural lubricant and its excellent water-retaining properties make it well-suited for use in ophthalmic products. The objective of this study is...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Atatürk University
2024-07-01
|
| Series: | Pharmata |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/4043433 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850141776759750656 |
|---|---|
| author | Durişehvar Ünal Merve Arslan |
| author_facet | Durişehvar Ünal Merve Arslan |
| author_sort | Durişehvar Ünal |
| collection | DOAJ |
| description | Objective: Hyaluronic acid (HA) is a naturally occurring, biocompatible polysaccharide with unique viscoelastic and hygroscopic properties. Its role as a natural lubricant and its excellent water-retaining properties make it well-suited for use in ophthalmic products. The objective of this study is determination of sodium hyaluronate in ophthalmic solution liquid chromatography (HPLC) method with UV detection and, stability of the product. Methods: The analysis was carried out using a polymer column (PolySep-GFC- P5000) with a mobile phase consisting of (dH2O: 20 mM Phosphate Buffer pH:6.5), (95:5, v:v) at a flow rate of 1.2 mL/min. UV detection was set 205 nm. Results: Calibration curve was linear over the concentration range of 80%-120% concentration of the ophthalmic product. The back calculated concentrations of the calibration standards were within ±15% of the nominal value (±20% for LOQ). The stability of ophtalmic solution was found to be 92.5% at the end of the days at room tempereture. Conclusion: The developed method is fast and reliable for HA analysis in ophthalmic products. Accordingt to stability study, these products must be stored at +4 C during usage and storage. |
| format | Article |
| id | doaj-art-18b2fbbb9b144112a7eb92393c16a42f |
| institution | OA Journals |
| issn | 2980-1966 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Atatürk University |
| record_format | Article |
| series | Pharmata |
| spelling | doaj-art-18b2fbbb9b144112a7eb92393c16a42f2025-08-20T02:29:19ZengAtatürk UniversityPharmata2980-19662024-07-0143778010.5281/zenodo.1262629255Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability AnalysisDurişehvar Ünal0https://orcid.org/0000-0003-0754-1240Merve Arslan1https://orcid.org/0000-0002-5758-9528ISTANBUL UNIVERSITY, FACULTY OF PHARMACYISTANBUL UNIVERSITY, FACULTY OF PHARMACYObjective: Hyaluronic acid (HA) is a naturally occurring, biocompatible polysaccharide with unique viscoelastic and hygroscopic properties. Its role as a natural lubricant and its excellent water-retaining properties make it well-suited for use in ophthalmic products. The objective of this study is determination of sodium hyaluronate in ophthalmic solution liquid chromatography (HPLC) method with UV detection and, stability of the product. Methods: The analysis was carried out using a polymer column (PolySep-GFC- P5000) with a mobile phase consisting of (dH2O: 20 mM Phosphate Buffer pH:6.5), (95:5, v:v) at a flow rate of 1.2 mL/min. UV detection was set 205 nm. Results: Calibration curve was linear over the concentration range of 80%-120% concentration of the ophthalmic product. The back calculated concentrations of the calibration standards were within ±15% of the nominal value (±20% for LOQ). The stability of ophtalmic solution was found to be 92.5% at the end of the days at room tempereture. Conclusion: The developed method is fast and reliable for HA analysis in ophthalmic products. Accordingt to stability study, these products must be stored at +4 C during usage and storage.https://dergipark.org.tr/en/download/article-file/4043433hyaluronic acidophthalmic producthplc |
| spellingShingle | Durişehvar Ünal Merve Arslan Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis Pharmata hyaluronic acid ophthalmic product hplc |
| title | Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis |
| title_full | Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis |
| title_fullStr | Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis |
| title_full_unstemmed | Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis |
| title_short | Determination of Hyaluronic Acid in Ophthalmic Solution by HPLC–UV and Stability Analysis |
| title_sort | determination of hyaluronic acid in ophthalmic solution by hplc uv and stability analysis |
| topic | hyaluronic acid ophthalmic product hplc |
| url | https://dergipark.org.tr/en/download/article-file/4043433 |
| work_keys_str_mv | AT durisehvarunal determinationofhyaluronicacidinophthalmicsolutionbyhplcuvandstabilityanalysis AT mervearslan determinationofhyaluronicacidinophthalmicsolutionbyhplcuvandstabilityanalysis |