Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions
Mitochondria are central coordinators of energy metabolism, and changes of their physiology have long been associated with metabolic disorders. Thus, observations of energy dynamics in different cell types are of utmost importance. Therefore, tools with quick and easy handling are needed for consist...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/629326 |
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| author | Erika Cortez Fabiana A. Neves Amélia F. Bernardo Ana Carolina Stumbo Laís Carvalho Érica Garcia-Souza Rosely Sichieri Anibal S. Moura |
| author_facet | Erika Cortez Fabiana A. Neves Amélia F. Bernardo Ana Carolina Stumbo Laís Carvalho Érica Garcia-Souza Rosely Sichieri Anibal S. Moura |
| author_sort | Erika Cortez |
| collection | DOAJ |
| description | Mitochondria are central coordinators of energy metabolism, and changes of their physiology have long been associated with metabolic disorders. Thus, observations of energy dynamics in different cell types are of utmost importance. Therefore, tools with quick and easy handling are needed for consistent evaluations of such interventions. In this paper, our main hypothesis is that during different nutritional situations lymphocytes mitochondrial physiology could be associated with the metabolism of other cell types, such as cardiomyocytes, and consequently be used as metabolic biomarker. Blood lymphocytes and heart muscle fibers were obtained from both fed and 24 h-fasted mice, and mitochondrial analysis was assessed by high-resolution respirometry and western blotting. Carbohydrate-linked oxidation and fatty acid oxidation were significantly higher after fasting. Carnitine palmitoil transferase 1 and uncouple protein 2 contents were increased in the fasted group, while the glucose transporters 1 and 4 and the ratio phosphorylated AMP-activated protein kinase/AMPK did not change between groups. In summary, under a nutritional status modification, mitochondria demonstrated earlier adaptive capacity than other metabolic sensors such as glucose transporters and AMPK, suggesting the accuracy of mitochondria physiology of lymphocytes as biomarker for metabolic changes. |
| format | Article |
| id | doaj-art-189fb280b7b64904a5c1bdc6dcf65114 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-189fb280b7b64904a5c1bdc6dcf651142025-08-20T03:54:12ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/629326629326Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed ConditionsErika Cortez0Fabiana A. Neves1Amélia F. Bernardo2Ana Carolina Stumbo3Laís Carvalho4Érica Garcia-Souza5Rosely Sichieri6Anibal S. Moura7Laboratory of Physiology of Nutrition and Development, Department of Physiological Sciences, Institute of Biology, Rio de Janeiro State University, Avenue 28 de Setembro 87, 5th Floor, Vila Isabel, 20551-030 Rio de Janeiro, RJ, BrazilLaboratory of Physiology of Nutrition and Development, Department of Physiological Sciences, Institute of Biology, Rio de Janeiro State University, Avenue 28 de Setembro 87, 5th Floor, Vila Isabel, 20551-030 Rio de Janeiro, RJ, BrazilLaboratory of Physiology of Nutrition and Development, Department of Physiological Sciences, Institute of Biology, Rio de Janeiro State University, Avenue 28 de Setembro 87, 5th Floor, Vila Isabel, 20551-030 Rio de Janeiro, RJ, BrazilLaboratory of Cell Culture, Department of Histology and Embryology, Institute of Biology, Rio de Janeiro State University, 20551-030 Rio de Janeiro, RJ, BrazilLaboratory of Cell Culture, Department of Histology and Embryology, Institute of Biology, Rio de Janeiro State University, 20551-030 Rio de Janeiro, RJ, BrazilLaboratory of Physiology of Nutrition and Development, Department of Physiological Sciences, Institute of Biology, Rio de Janeiro State University, Avenue 28 de Setembro 87, 5th Floor, Vila Isabel, 20551-030 Rio de Janeiro, RJ, BrazilDepartment of Epidemiology, Institute of Social Medicine, Rio de Janeiro State University, 20551-030 Rio de Janeiro, RJ, BrazilLaboratory of Physiology of Nutrition and Development, Department of Physiological Sciences, Institute of Biology, Rio de Janeiro State University, Avenue 28 de Setembro 87, 5th Floor, Vila Isabel, 20551-030 Rio de Janeiro, RJ, BrazilMitochondria are central coordinators of energy metabolism, and changes of their physiology have long been associated with metabolic disorders. Thus, observations of energy dynamics in different cell types are of utmost importance. Therefore, tools with quick and easy handling are needed for consistent evaluations of such interventions. In this paper, our main hypothesis is that during different nutritional situations lymphocytes mitochondrial physiology could be associated with the metabolism of other cell types, such as cardiomyocytes, and consequently be used as metabolic biomarker. Blood lymphocytes and heart muscle fibers were obtained from both fed and 24 h-fasted mice, and mitochondrial analysis was assessed by high-resolution respirometry and western blotting. Carbohydrate-linked oxidation and fatty acid oxidation were significantly higher after fasting. Carnitine palmitoil transferase 1 and uncouple protein 2 contents were increased in the fasted group, while the glucose transporters 1 and 4 and the ratio phosphorylated AMP-activated protein kinase/AMPK did not change between groups. In summary, under a nutritional status modification, mitochondria demonstrated earlier adaptive capacity than other metabolic sensors such as glucose transporters and AMPK, suggesting the accuracy of mitochondria physiology of lymphocytes as biomarker for metabolic changes.http://dx.doi.org/10.1100/2012/629326 |
| spellingShingle | Erika Cortez Fabiana A. Neves Amélia F. Bernardo Ana Carolina Stumbo Laís Carvalho Érica Garcia-Souza Rosely Sichieri Anibal S. Moura Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions The Scientific World Journal |
| title | Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions |
| title_full | Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions |
| title_fullStr | Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions |
| title_full_unstemmed | Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions |
| title_short | Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions |
| title_sort | lymphocytes mitochondrial physiology as biomarker of energy metabolism during fasted and fed conditions |
| url | http://dx.doi.org/10.1100/2012/629326 |
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