Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment
Introduction: Chicken farms are nowadays regarded as reservoirs of multi-drug resistance. Studies have shown that resistant organisms can be readily transferred from animals to their surrounding ecosystem. The aim of this study is to determine if any link exists between the prevalence of multi-drug...
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The Journal of Infection in Developing Countries
2018-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10041 |
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| author | Iman Dandachi Elie Fayad Bassel El-Bazzal Ahmad Sleiman Jean-Marc Rolain Ziad Daoud |
| author_facet | Iman Dandachi Elie Fayad Bassel El-Bazzal Ahmad Sleiman Jean-Marc Rolain Ziad Daoud |
| author_sort | Iman Dandachi |
| collection | DOAJ |
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Introduction: Chicken farms are nowadays regarded as reservoirs of multi-drug resistance. Studies have shown that resistant organisms can be readily transferred from animals to their surrounding ecosystem. The aim of this study is to determine if any link exists between the prevalence of multi-drug resistance in chicken farms and their surrounding environment.
Methodology: In May-2017, 200 fecal swabs were collected from a chicken farm in Lebanon. Fecal samples from six workers and 41 environmental samples surrounding the farm were also taken. Three different selective media were used for the screening of multi-drug resistant and colistin resistant organisms. MALDI-TOF was used for bacterial identification. Double disk synergy test and ampC disk test were used for the screening of ESBL and ampC producers respectively. Furthermore, RT-PCR was performed for the detection of beta lactamase and mcr colistin resistance genes.
Results: In chicken, 315 E.coli strains were isolated: 53% were ESBL/ampC co-producers, 27% ampC and 42.5% mcr-1 positive isolates. Furthermore, 29 K.pneumoniae harboring mcr-1 were also isolated. In workers, ESBL producing E.coli were detected in 4/6 workers whereas mcr-1 carrying E.coli were detected in all workers. In the environment, ESBLs and mcr-1 positives were detected in 95% and 7% of the samples respectively. RT-PCR revealed the detection of B-lactamase genes in all samples at different rates.
Conclusions: This study showed a relatively high prevalence of ESBL and mcr-1 positive isolates in chicken and their environment. MLST is in progress to determine if any link exists between multi-drug resistant organisms in these ecosystems investigated.
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| format | Article |
| id | doaj-art-18902bdbef4043b9b505deb78f9bc1f4 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-18902bdbef4043b9b505deb78f9bc1f42025-08-20T03:52:43ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-02-011202.110.3855/jidc.10041Multi Drug resistant Organisms in Chicken farms and their Surrounding EnvironmentIman Dandachi0Elie Fayad1Bassel El-Bazzal2Ahmad Sleiman3Jean-Marc Rolain4Ziad Daoud5Clinical Microbiology Laboratory, Faculty of Medicine and Medical Sciences, University of Balamand, Amioun, Beirut, LebanonClinical Microbiology Laboratory, Faculty of Medicine and Medical Sciences, University of Balamand, Amioun, Beirut, LebanonMinistry of Agriculture, Beirut , LebanonClinical Microbiology Laboratory, Faculty of Medicine and Medical Sciences, University of Balamand, Amioun, Beirut, LebanonUnité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Univ, Marseille, FranceClinical Microbiology Laboratory, Faculty of Medicine and Medical Sciences, University of Balamand, Amioun, Beirut, Lebanon Introduction: Chicken farms are nowadays regarded as reservoirs of multi-drug resistance. Studies have shown that resistant organisms can be readily transferred from animals to their surrounding ecosystem. The aim of this study is to determine if any link exists between the prevalence of multi-drug resistance in chicken farms and their surrounding environment. Methodology: In May-2017, 200 fecal swabs were collected from a chicken farm in Lebanon. Fecal samples from six workers and 41 environmental samples surrounding the farm were also taken. Three different selective media were used for the screening of multi-drug resistant and colistin resistant organisms. MALDI-TOF was used for bacterial identification. Double disk synergy test and ampC disk test were used for the screening of ESBL and ampC producers respectively. Furthermore, RT-PCR was performed for the detection of beta lactamase and mcr colistin resistance genes. Results: In chicken, 315 E.coli strains were isolated: 53% were ESBL/ampC co-producers, 27% ampC and 42.5% mcr-1 positive isolates. Furthermore, 29 K.pneumoniae harboring mcr-1 were also isolated. In workers, ESBL producing E.coli were detected in 4/6 workers whereas mcr-1 carrying E.coli were detected in all workers. In the environment, ESBLs and mcr-1 positives were detected in 95% and 7% of the samples respectively. RT-PCR revealed the detection of B-lactamase genes in all samples at different rates. Conclusions: This study showed a relatively high prevalence of ESBL and mcr-1 positive isolates in chicken and their environment. MLST is in progress to determine if any link exists between multi-drug resistant organisms in these ecosystems investigated. https://jidc.org/index.php/journal/article/view/10041mcr-1chickenESBL |
| spellingShingle | Iman Dandachi Elie Fayad Bassel El-Bazzal Ahmad Sleiman Jean-Marc Rolain Ziad Daoud Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment Journal of Infection in Developing Countries mcr-1 chicken ESBL |
| title | Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment |
| title_full | Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment |
| title_fullStr | Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment |
| title_full_unstemmed | Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment |
| title_short | Multi Drug resistant Organisms in Chicken farms and their Surrounding Environment |
| title_sort | multi drug resistant organisms in chicken farms and their surrounding environment |
| topic | mcr-1 chicken ESBL |
| url | https://jidc.org/index.php/journal/article/view/10041 |
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