Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood
Abstract Although chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy against leukemic cells, it still has critical limitations. CAR macrophage has been regarded as a potential alternative to CAR T cells. However, due to the difficulties in gene transduction into macrophages...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Biomarker Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40364-024-00703-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559192253497344 |
|---|---|
| author | Ji U Choi Yeongrin Kim Da Yeon Lee Jin Song Park Moonjung Jeun Heung Kyoung Lee Chi Hoon Park |
| author_facet | Ji U Choi Yeongrin Kim Da Yeon Lee Jin Song Park Moonjung Jeun Heung Kyoung Lee Chi Hoon Park |
| author_sort | Ji U Choi |
| collection | DOAJ |
| description | Abstract Although chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy against leukemic cells, it still has critical limitations. CAR macrophage has been regarded as a potential alternative to CAR T cells. However, due to the difficulties in gene transduction into macrophages, the production of primary human CAR macrophages from peripheral blood mononuclear cells (PBMC) using lentivirus is highly challenging. Here, we report on how to generate CAR macrophages from human PBMC with lentiviral particles. Using our lentiviral protocol, we produced functional CAR macrophages to lyse and phagocytose target cancer cells efficiently. |
| format | Article |
| id | doaj-art-188c7d6ddb2e4cf7a153fa09fb12a26d |
| institution | Kabale University |
| issn | 2050-7771 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biomarker Research |
| spelling | doaj-art-188c7d6ddb2e4cf7a153fa09fb12a26d2025-01-05T12:42:02ZengBMCBiomarker Research2050-77712025-01-011311510.1186/s40364-024-00703-9Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral bloodJi U Choi0Yeongrin Kim1Da Yeon Lee2Jin Song Park3Moonjung Jeun4Heung Kyoung Lee5Chi Hoon Park6Bio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyBio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyBio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyBio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyBio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyBio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyBio & Drug Discovery Division, Korea Research Institute of Chemical TechnologyAbstract Although chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy against leukemic cells, it still has critical limitations. CAR macrophage has been regarded as a potential alternative to CAR T cells. However, due to the difficulties in gene transduction into macrophages, the production of primary human CAR macrophages from peripheral blood mononuclear cells (PBMC) using lentivirus is highly challenging. Here, we report on how to generate CAR macrophages from human PBMC with lentiviral particles. Using our lentiviral protocol, we produced functional CAR macrophages to lyse and phagocytose target cancer cells efficiently.https://doi.org/10.1186/s40364-024-00703-9CAR macrophagesLentivirusHuman PBMCPrimary human macrophages |
| spellingShingle | Ji U Choi Yeongrin Kim Da Yeon Lee Jin Song Park Moonjung Jeun Heung Kyoung Lee Chi Hoon Park Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood Biomarker Research CAR macrophages Lentivirus Human PBMC Primary human macrophages |
| title | Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood |
| title_full | Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood |
| title_fullStr | Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood |
| title_full_unstemmed | Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood |
| title_short | Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood |
| title_sort | lentivirus based production of human chimeric antigen receptor macrophages from peripheral blood |
| topic | CAR macrophages Lentivirus Human PBMC Primary human macrophages |
| url | https://doi.org/10.1186/s40364-024-00703-9 |
| work_keys_str_mv | AT jiuchoi lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood AT yeongrinkim lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood AT dayeonlee lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood AT jinsongpark lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood AT moonjungjeun lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood AT heungkyounglee lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood AT chihoonpark lentivirusbasedproductionofhumanchimericantigenreceptormacrophagesfromperipheralblood |