The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM)
Abstract TGF-β1 has been reported to suppress miR-130a expression, while being elevated in patients with type 2 diabetes mellitus (T2DM). And IL-18, a potential target of miR-130a, is also up-regulated in T2DM patients. In this study, we aim to investigate the potential involvement of the TGFβ1/miR-...
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Nature Portfolio
2025-04-01
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| author | Yawei Cai Yao Zhu Ning Xu Tongen Chen |
| author_facet | Yawei Cai Yao Zhu Ning Xu Tongen Chen |
| author_sort | Yawei Cai |
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| description | Abstract TGF-β1 has been reported to suppress miR-130a expression, while being elevated in patients with type 2 diabetes mellitus (T2DM). And IL-18, a potential target of miR-130a, is also up-regulated in T2DM patients. In this study, we aim to investigate the potential involvement of the TGFβ1/miR-130a/IL-18 axis underlying the dysfunction of endothelial progenitor cells (EPCs) in T2DM patients. We performed luciferase assays to confirm the molecular binding between miR-130a, TGF-β1 and IL-18, and real-time PCR and ELISA were performed to observe the changes of TGF-β1, miR-130a, IL-18 and IFN-γ in different cell groups. Tube formation assay, cell adhesion assay and Transwell assay were performed to evaluate effect of TGF-β1/miR-130a/IL-18 axis on the EPCs functions. Accordingly, in EPCs treated with TGF-β1, we found that the levels of miR-130a were reduced, and its expressions were also negatively correlated with the expressions of IL-18 in the EPC groups. Luciferase assays validated IL-18 as a target gene of miR-130a. The over-expression of IL-18, as well as the knockdown of miR-130a, not only increased the expressions of TGF-β1 in EPCs, but also promoted the tube formation, adhesion and migration of EPCs. By contrast, the knockdown of IL-18, as well as the over-regulation of miR-130a, exhibited suppressive effect on the levels of TGF-β1, while inhibiting the tube formation, adhesion and migration of EPCs. In this study, we elucidated the impact of the TGFβ1/miR-130a/IL-18 signaling pathway on the function of EPCs, thus providing theoretical basis for the development of miRNA-targeted therapeutic strategies for patients withT2DM and associated complications. |
| format | Article |
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| institution | DOAJ |
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| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-1879ba7caf1b4df59d3e0fde53ceecac2025-08-20T03:07:41ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-88507-1The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM)Yawei Cai0Yao Zhu1Ning Xu2Tongen Chen3Department of Geriatrics, Ningbo No. 2 HospitalDepartment of Geriatrics, Ningbo No. 2 HospitalDepartment of Geriatrics, Ningbo No. 2 HospitalDepartment of General Practice, Ningbo No. 2 HospitalAbstract TGF-β1 has been reported to suppress miR-130a expression, while being elevated in patients with type 2 diabetes mellitus (T2DM). And IL-18, a potential target of miR-130a, is also up-regulated in T2DM patients. In this study, we aim to investigate the potential involvement of the TGFβ1/miR-130a/IL-18 axis underlying the dysfunction of endothelial progenitor cells (EPCs) in T2DM patients. We performed luciferase assays to confirm the molecular binding between miR-130a, TGF-β1 and IL-18, and real-time PCR and ELISA were performed to observe the changes of TGF-β1, miR-130a, IL-18 and IFN-γ in different cell groups. Tube formation assay, cell adhesion assay and Transwell assay were performed to evaluate effect of TGF-β1/miR-130a/IL-18 axis on the EPCs functions. Accordingly, in EPCs treated with TGF-β1, we found that the levels of miR-130a were reduced, and its expressions were also negatively correlated with the expressions of IL-18 in the EPC groups. Luciferase assays validated IL-18 as a target gene of miR-130a. The over-expression of IL-18, as well as the knockdown of miR-130a, not only increased the expressions of TGF-β1 in EPCs, but also promoted the tube formation, adhesion and migration of EPCs. By contrast, the knockdown of IL-18, as well as the over-regulation of miR-130a, exhibited suppressive effect on the levels of TGF-β1, while inhibiting the tube formation, adhesion and migration of EPCs. In this study, we elucidated the impact of the TGFβ1/miR-130a/IL-18 signaling pathway on the function of EPCs, thus providing theoretical basis for the development of miRNA-targeted therapeutic strategies for patients withT2DM and associated complications.https://doi.org/10.1038/s41598-025-88507-1DiabetesType 2 diabetes mellitusEndothelial progenitor cellsMiRNAPeripheral blood |
| spellingShingle | Yawei Cai Yao Zhu Ning Xu Tongen Chen The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM) Scientific Reports Diabetes Type 2 diabetes mellitus Endothelial progenitor cells MiRNA Peripheral blood |
| title | The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM) |
| title_full | The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM) |
| title_fullStr | The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM) |
| title_full_unstemmed | The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM) |
| title_short | The study of miR-130a expression and its mechanism of action in peripheral blood endothelial progenitor cells (EPCs) in type 2 diabetes mellitus (T2DM) |
| title_sort | study of mir 130a expression and its mechanism of action in peripheral blood endothelial progenitor cells epcs in type 2 diabetes mellitus t2dm |
| topic | Diabetes Type 2 diabetes mellitus Endothelial progenitor cells MiRNA Peripheral blood |
| url | https://doi.org/10.1038/s41598-025-88507-1 |
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