Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems
Soil microbiomes play pivotal roles in mediating plant diversity maintenance by regulating multifunctional ecosystem services during plant development. However, how different stand age of plants influence soil microbial communities in various soil compartments remains poorly understood. Through Illu...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1626892/full |
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| author | Bin He Bin He Ping Zhang Xiaolong Bai Xiaolong Bai Wangjun Li Wangjun Li Shun Zou Shun Zou |
| author_facet | Bin He Bin He Ping Zhang Xiaolong Bai Xiaolong Bai Wangjun Li Wangjun Li Shun Zou Shun Zou |
| author_sort | Bin He |
| collection | DOAJ |
| description | Soil microbiomes play pivotal roles in mediating plant diversity maintenance by regulating multifunctional ecosystem services during plant development. However, how different stand age of plants influence soil microbial communities in various soil compartments remains poorly understood. Through Illumina-based 16S rRNA and ITS amplicon sequencing, we systematically investigated the successional trajectories of soil microbiome in Pinus armandii plantations spanning various developmental phases. Key findings revealed that stand age exerted a stronger influence on microbial restructuring than soil compartment, significantly altering community composition in both soil types. Alpha diversity (Shannon and Chao1 indices) exhibited a U-shaped trajectory with stand age, except for fungal Chao1 in bulk soil. While dominant bacterial and fungal phyla remained relatively stable, community composition displayed significant stage-dependent variations. Co-occurrence network analysis demonstrated lower fungal network complexity compared to bacterial networks, with rhizosphere soils harboring more intricate interactions compared to bulk soils. Community assembly mechanisms diverged: deterministic processes dominated bacterial assembly, whereas stochasticity governed fungal communities. Soil properties exerted significant influences on microbial composition and diversity: bacterial composition correlated strongly with pH and stoichiometric ratios (C/N, C/P, N/P), while fungal composition showed stronger associations with TN, TP, and AN. Our results demonstrate that P. armandii plantations maintain core phylum-level microbial populations while developing stage-specific diversity patterns. Crucially, bacteria and fungi exhibit divergent responses to stand development, highlighting their divergent ecological strategies in adapting to nutrient-limited karst ecosystems. |
| format | Article |
| id | doaj-art-18729346c16e40f6884df5959d40a9fe |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-18729346c16e40f6884df5959d40a9fe2025-08-20T02:37:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16268921626892Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystemsBin He0Bin He1Ping Zhang2Xiaolong Bai3Xiaolong Bai4Wangjun Li5Wangjun Li6Shun Zou7Shun Zou8College of Ecological Engineering, Guizhou University of Engineering Science, Bijie, ChinaGuizhou Province Key Laboratory of Ecological Protection and Restoration of Typical Plateau Wetlands, Bijie, ChinaCollege of Ecological Engineering, Guizhou University of Engineering Science, Bijie, ChinaCollege of Ecological Engineering, Guizhou University of Engineering Science, Bijie, ChinaGuizhou Province Key Laboratory of Ecological Protection and Restoration of Typical Plateau Wetlands, Bijie, ChinaCollege of Ecological Engineering, Guizhou University of Engineering Science, Bijie, ChinaGuizhou Province Key Laboratory of Ecological Protection and Restoration of Typical Plateau Wetlands, Bijie, ChinaCollege of Ecological Engineering, Guizhou University of Engineering Science, Bijie, ChinaGuizhou Province Key Laboratory of Ecological Protection and Restoration of Typical Plateau Wetlands, Bijie, ChinaSoil microbiomes play pivotal roles in mediating plant diversity maintenance by regulating multifunctional ecosystem services during plant development. However, how different stand age of plants influence soil microbial communities in various soil compartments remains poorly understood. Through Illumina-based 16S rRNA and ITS amplicon sequencing, we systematically investigated the successional trajectories of soil microbiome in Pinus armandii plantations spanning various developmental phases. Key findings revealed that stand age exerted a stronger influence on microbial restructuring than soil compartment, significantly altering community composition in both soil types. Alpha diversity (Shannon and Chao1 indices) exhibited a U-shaped trajectory with stand age, except for fungal Chao1 in bulk soil. While dominant bacterial and fungal phyla remained relatively stable, community composition displayed significant stage-dependent variations. Co-occurrence network analysis demonstrated lower fungal network complexity compared to bacterial networks, with rhizosphere soils harboring more intricate interactions compared to bulk soils. Community assembly mechanisms diverged: deterministic processes dominated bacterial assembly, whereas stochasticity governed fungal communities. Soil properties exerted significant influences on microbial composition and diversity: bacterial composition correlated strongly with pH and stoichiometric ratios (C/N, C/P, N/P), while fungal composition showed stronger associations with TN, TP, and AN. Our results demonstrate that P. armandii plantations maintain core phylum-level microbial populations while developing stage-specific diversity patterns. Crucially, bacteria and fungi exhibit divergent responses to stand development, highlighting their divergent ecological strategies in adapting to nutrient-limited karst ecosystems.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1626892/fullkarst mountain ecosystemssoil microbial communitycompartment-specific dynamicsstand age chronosequenceplantation |
| spellingShingle | Bin He Bin He Ping Zhang Xiaolong Bai Xiaolong Bai Wangjun Li Wangjun Li Shun Zou Shun Zou Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems Frontiers in Microbiology karst mountain ecosystems soil microbial community compartment-specific dynamics stand age chronosequence plantation |
| title | Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems |
| title_full | Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems |
| title_fullStr | Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems |
| title_full_unstemmed | Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems |
| title_short | Compartment-specific dynamics of soil microbiota along a Pinus armandii plantation chronosequence in karst mountain ecosystems |
| title_sort | compartment specific dynamics of soil microbiota along a pinus armandii plantation chronosequence in karst mountain ecosystems |
| topic | karst mountain ecosystems soil microbial community compartment-specific dynamics stand age chronosequence plantation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1626892/full |
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