Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana

WRKY transcription factors are a class of DNA-binding proteins that bind with a specific sequence C/TTGACT/C known as W-Box found in promoters of genes which are regulated by these WRKYs. From previous studies, 43 different stress responsive WRKY transcription factors in Arabidopsis thaliana, identi...

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Main Authors: Shaiq Sultan, Muhammad Amjid Ali, Rana Muhammad Atif, Farrukh Azeem, Habibullah Nadeem, M. Hussnain Siddique, Ertuğrul Filiz, Khadim Hussain, Amjad Abbas
Format: Article
Language:English
Published: Hasan Eleroğlu 2016-04-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/580
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author Shaiq Sultan
Muhammad Amjid Ali
Rana Muhammad Atif
Farrukh Azeem
Habibullah Nadeem
M. Hussnain Siddique
Ertuğrul Filiz
Khadim Hussain
Amjad Abbas
author_facet Shaiq Sultan
Muhammad Amjid Ali
Rana Muhammad Atif
Farrukh Azeem
Habibullah Nadeem
M. Hussnain Siddique
Ertuğrul Filiz
Khadim Hussain
Amjad Abbas
author_sort Shaiq Sultan
collection DOAJ
description WRKY transcription factors are a class of DNA-binding proteins that bind with a specific sequence C/TTGACT/C known as W-Box found in promoters of genes which are regulated by these WRKYs. From previous studies, 43 different stress responsive WRKY transcription factors in Arabidopsis thaliana, identified and then categorized in three groups viz., abiotic, biotic and both of these stresses. A comprehensive genome wide analysis including chromosomal localization, gene structure analysis, multiple sequence alignment, phylogenetic analysis and promoter analysis of these WRKY genes was carried out in this study to determine the functional homology in Arabidopsis. This analysis led to the classification of these WRKY family members into 3 major groups and subgroups and showed evolutionary relationship among these groups on the base of their functional WRKY domain, chromosomal localization and intron/exon structure. The proposed groups of these stress responsive WRKY genes and annotation based on their position on chromosomes can also be explored to determine their functional homology in other plant species in relation to different stresses. The result of the present study provides indispensable genomic information for the stress responsive WRKY transcription factors in Arabidopsis and will pave the way to explain the precise role of various AtWRKYs in plant growth and development under stressed conditions.
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publisher Hasan Eleroğlu
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spelling doaj-art-186969ec7855413289a2ff7b66112b272025-08-20T02:02:16ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2016-04-014427929010.24925/turjaf.v4i4.279-290.580301Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thalianaShaiq Sultan0Muhammad Amjid Ali1Rana Muhammad Atif2Farrukh Azeem3Habibullah Nadeem4M. Hussnain Siddique5Ertuğrul Filiz6Khadim Hussain7Amjad Abbas8Department of Bioinformatics & Biotechnology, Government College University, 38000 FaisalabadDepartment of Plant Pathology, University of Agriculture, 38040 Faisalabad,Centre of Agricultural Biochemistry & Biotechnology, University of Agriculture, 38040 Faisalabad,Department of Bioinformatics & Biotechnology, Government College University, 38000 FaisalabadDepartment of Bioinformatics & Biotechnology, Government College University, 38000 FaisalabadDepartment of Bioinformatics & Biotechnology, Government College University, 38000 FaisalabadDepartment of Crop and Animal Production, Cilimli Vocational School, Duzce University, 81750 Cilimli/DuzceDepartment of Bioinformatics & Biotechnology, Government College University, 38000 FaisalabadDepartment of Plant Pathology, University of Agriculture, 38040 FaisalabadWRKY transcription factors are a class of DNA-binding proteins that bind with a specific sequence C/TTGACT/C known as W-Box found in promoters of genes which are regulated by these WRKYs. From previous studies, 43 different stress responsive WRKY transcription factors in Arabidopsis thaliana, identified and then categorized in three groups viz., abiotic, biotic and both of these stresses. A comprehensive genome wide analysis including chromosomal localization, gene structure analysis, multiple sequence alignment, phylogenetic analysis and promoter analysis of these WRKY genes was carried out in this study to determine the functional homology in Arabidopsis. This analysis led to the classification of these WRKY family members into 3 major groups and subgroups and showed evolutionary relationship among these groups on the base of their functional WRKY domain, chromosomal localization and intron/exon structure. The proposed groups of these stress responsive WRKY genes and annotation based on their position on chromosomes can also be explored to determine their functional homology in other plant species in relation to different stresses. The result of the present study provides indispensable genomic information for the stress responsive WRKY transcription factors in Arabidopsis and will pave the way to explain the precise role of various AtWRKYs in plant growth and development under stressed conditions.http://www.agrifoodscience.com/index.php/TURJAF/article/view/580WRKY, Arabidopsis thaliana, stress, W-Box, phylogenetic analysis, transcription factor
spellingShingle Shaiq Sultan
Muhammad Amjid Ali
Rana Muhammad Atif
Farrukh Azeem
Habibullah Nadeem
M. Hussnain Siddique
Ertuğrul Filiz
Khadim Hussain
Amjad Abbas
Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana
Turkish Journal of Agriculture: Food Science and Technology
WRKY, Arabidopsis thaliana, stress, W-Box, phylogenetic analysis, transcription factor
title Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana
title_full Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana
title_fullStr Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana
title_full_unstemmed Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana
title_short Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana
title_sort genome wide analysis of stress responsive wrky transcription factors in arabidopsis thaliana
topic WRKY, Arabidopsis thaliana, stress, W-Box, phylogenetic analysis, transcription factor
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/580
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