Analysis of diagnostic parameters of Truenat HPV for detecting Human papilloma virus: A study from a tertiary care hospital
Background: Human Papillomavirus (HPV) is a major etiological factor in cervical cancer, making rapid and accurate detection crucial for diagnosis. Prolonged processing times, high costs, and advanced infrastructures are the drawbacks of conventional diagnostic techniques. Aim and Objectives: To co...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Krishna Vishwa Vidyapeeth (Deemed to be University), Karad
2025-01-01
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| Series: | Journal of Krishna Institute of Medical Sciences University |
| Subjects: | |
| Online Access: | https://www.jkimsu.com/jkimsu-vol14no1/JKIMSU,%20Vol.%2014,%20No.%201,%20January-March%202025%20Page%2057-65.pdf |
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| Summary: | Background: Human Papillomavirus (HPV) is a major etiological factor in cervical cancer, making rapid and accurate detection crucial for diagnosis. Prolonged processing times, high costs, and advanced infrastructures are the drawbacks
of conventional diagnostic techniques. Aim and Objectives: To compare the diagnostic performance of the portable, chip-based Truenat HPV-HR kit with TRUPCR HPV real-time polymerase chain reaction (RT-PCR) and Pap smear
tests. Material and Methods: Cervical swabs were obtained from women aged 20-69 years for a comparative diagnostic study for the detection of HPV. The diagnostic parameters were computed using SPSS version 29.0.2.0 and Microsoft Excel. Concordance between the methods was calculated using Cohen's kappa coefficient. Results: HPV was detected in 12.07% using TRUPCR HPV and 5.17% using Truenat HPV-HR. Truenat showed a sensitivity and specificity of 42.86% and 100% for 16/31 and 18/45 HPV genotypes, respectively. Concordance between the methods was 93.1% (κ = 0.57), indicating moderate agreement. HPV prevalence was highest among individuals aged 31–40 years (42.86%) and in women with normal cytology (15.38%). Conclusion: The Truenat HPV-HR test showed significant concurrence with TRUPCR HPV, although no association with cytological results was found. This suggests that incorporating PCR could potentially improve early diagnosis, bridge diagnostic gaps, and enhance patient outcomes. |
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| ISSN: | 2231-4261 |