The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression
Background: Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cancer. 7-Methylguanosine (m7G), one of the most prevalent RNA modifications, has been reported to play an important role in ccRCC progression; however, the specific regulators of m7G modification that are involv...
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Elsevier
2025-01-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523324003280 |
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| author | Yi Liu Yanji Zhan Jiao Liu Zhengze Shen Yudong Hu Ling Zhong Yuan Yu Bin Tang Jing Guo |
| author_facet | Yi Liu Yanji Zhan Jiao Liu Zhengze Shen Yudong Hu Ling Zhong Yuan Yu Bin Tang Jing Guo |
| author_sort | Yi Liu |
| collection | DOAJ |
| description | Background: Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cancer. 7-Methylguanosine (m7G), one of the most prevalent RNA modifications, has been reported to play an important role in ccRCC progression; however, the specific regulators of m7G modification that are involved in this function remain unclear. This study aimed to explore the correlation between regulators of m7G methylation and ccRCC progression using unsupervised machine learning methods.Methods: Transcriptome and clinical data of ccRCC were retrieved from The Cancer Genome Atlas (TCGA) database to identify differentially expressed m7G-related genes associated with the overall survival of patients with ccRCC. To construct and validate a prognostic risk model, TCGA dataset samples were divided into training and test sets. A multiple-gene risk signature was constructed using least absolute shrinkage and selection operator Cox regression analysis, and its prognostic significance was assessed using Cox regression and survival analyses. Finally, immunohistochemistry was performed to verify the prognostic significance of this signature.Results: In total, 537 patients with ccRCC were included in this study. We found that 26 m7G RNA methylation regulators that were significantly differentially expressed. Univariate and multifactorial Cox regression analyses revealed that METTL1 expression was associated with ccRCC progression.Conclusions: METTL1 associated with m7G may serve as a potential biomarker for ccRCC prognosis and diagnosis. Moreover, it may affect the prognosis of ccRCC by regulating the tumor immune microenvironment, providing a potential therapeutic target for immunotherapy. These results provide a new perspective on the role of M7G-related RNAs in ccRCC pathogenesis. |
| format | Article |
| id | doaj-art-1858fd01a5e543fc8dd07de45bc6bda2 |
| institution | OA Journals |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-1858fd01a5e543fc8dd07de45bc6bda22025-08-20T02:20:40ZengElsevierTranslational Oncology1936-52332025-01-015110220210.1016/j.tranon.2024.102202The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progressionYi Liu0Yanji Zhan1Jiao Liu2Zhengze Shen3Yudong Hu4Ling Zhong5Yuan Yu6Bin Tang7Jing Guo8Department of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China; The 3rd Affiliated Hospital, Chengdu Medical College, Chengdu Pidu District People's Hospital, Chengdu, 611730, ChinaDepartment of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, ChinaDepartment of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China; Department of Nephrology, Wushan County Peopleʼs Hospital of Chongqing, 404700, ChinaDepartment of Pharmacy, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160, ChinaDepartment of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, ChinaDepartment of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, ChinaDepartment of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, ChinaDepartment of Nephrology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China; Corresponding authors.Radiation Oncology Center, Chongqing University Cancer Hospital, Chongqing University, Chongqing 400030, China; Corresponding authors.Background: Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cancer. 7-Methylguanosine (m7G), one of the most prevalent RNA modifications, has been reported to play an important role in ccRCC progression; however, the specific regulators of m7G modification that are involved in this function remain unclear. This study aimed to explore the correlation between regulators of m7G methylation and ccRCC progression using unsupervised machine learning methods.Methods: Transcriptome and clinical data of ccRCC were retrieved from The Cancer Genome Atlas (TCGA) database to identify differentially expressed m7G-related genes associated with the overall survival of patients with ccRCC. To construct and validate a prognostic risk model, TCGA dataset samples were divided into training and test sets. A multiple-gene risk signature was constructed using least absolute shrinkage and selection operator Cox regression analysis, and its prognostic significance was assessed using Cox regression and survival analyses. Finally, immunohistochemistry was performed to verify the prognostic significance of this signature.Results: In total, 537 patients with ccRCC were included in this study. We found that 26 m7G RNA methylation regulators that were significantly differentially expressed. Univariate and multifactorial Cox regression analyses revealed that METTL1 expression was associated with ccRCC progression.Conclusions: METTL1 associated with m7G may serve as a potential biomarker for ccRCC prognosis and diagnosis. Moreover, it may affect the prognosis of ccRCC by regulating the tumor immune microenvironment, providing a potential therapeutic target for immunotherapy. These results provide a new perspective on the role of M7G-related RNAs in ccRCC pathogenesis.http://www.sciencedirect.com/science/article/pii/S1936523324003280METTL17-methylguanosine (m7G)Clear cell renal cell carcinomaPrognosisTumor immune microenvironment |
| spellingShingle | Yi Liu Yanji Zhan Jiao Liu Zhengze Shen Yudong Hu Ling Zhong Yuan Yu Bin Tang Jing Guo The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression Translational Oncology METTL1 7-methylguanosine (m7G) Clear cell renal cell carcinoma Prognosis Tumor immune microenvironment |
| title | The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression |
| title_full | The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression |
| title_fullStr | The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression |
| title_full_unstemmed | The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression |
| title_short | The 7-Methylguanosine (m7G) methylation METTL1 acts as a potential biomarker of clear cell renal cell carcinoma progression |
| title_sort | 7 methylguanosine m7g methylation mettl1 acts as a potential biomarker of clear cell renal cell carcinoma progression |
| topic | METTL1 7-methylguanosine (m7G) Clear cell renal cell carcinoma Prognosis Tumor immune microenvironment |
| url | http://www.sciencedirect.com/science/article/pii/S1936523324003280 |
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