TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep.
The development of secondary hair follicles influences the quality of sheep wool. However, the mechanism by which proteins mediate the fetal development of secondary hair follicles remains unknown. In this study, the histomorphology of secondary hair follicles was analyzed over four stages of fetal...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0315637 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856802904670208 |
|---|---|
| author | Li-Xia Qiu Qian Yu Hua-Qian Zhou Wen-Hua Fan Jing-Jing Zheng Yong-Lin Yang Wen-Zhe Zhang Xin Cao Hua Yang |
| author_facet | Li-Xia Qiu Qian Yu Hua-Qian Zhou Wen-Hua Fan Jing-Jing Zheng Yong-Lin Yang Wen-Zhe Zhang Xin Cao Hua Yang |
| author_sort | Li-Xia Qiu |
| collection | DOAJ |
| description | The development of secondary hair follicles influences the quality of sheep wool. However, the mechanism by which proteins mediate the fetal development of secondary hair follicles remains unknown. In this study, the histomorphology of secondary hair follicles was analyzed over four stages of fetal development (75, 85, 95, and 105 gestational days). TMT-based quantitative proteomics was used to compare the differential protein profiles of skin tissues between consecutive developmental periods (75 versus 85, 85 versus 95, and 95 versus 105 gestational days). We found that the density of secondary hair follicles and the secondary hair follicles/primary hair follicles ratio increased from 85 to 105 gestational days. Bioinformatic analysis identified 238, 35, and 348 differentially expressed proteins in the respective comparison periods. Focal adhesion, ECM-receptor interaction, and the estrogen signaling pathway all played important roles in secondary hair follicle development. COL1A1, THBS3, ITGA6, COL6A1, and THBS4 were identified as potential candidate proteins in the initiation of secondary hair follicles. This study provides valuable proteomics data on secondary hair follicle development and thus has deepened our understanding of the molecular mechanisms underlying wool quality traits in fine-wool sheep. |
| format | Article |
| id | doaj-art-1850d981956d4438b0bd5b03ff094c62 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1850d981956d4438b0bd5b03ff094c622025-02-12T05:30:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031563710.1371/journal.pone.0315637TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep.Li-Xia QiuQian YuHua-Qian ZhouWen-Hua FanJing-Jing ZhengYong-Lin YangWen-Zhe ZhangXin CaoHua YangThe development of secondary hair follicles influences the quality of sheep wool. However, the mechanism by which proteins mediate the fetal development of secondary hair follicles remains unknown. In this study, the histomorphology of secondary hair follicles was analyzed over four stages of fetal development (75, 85, 95, and 105 gestational days). TMT-based quantitative proteomics was used to compare the differential protein profiles of skin tissues between consecutive developmental periods (75 versus 85, 85 versus 95, and 95 versus 105 gestational days). We found that the density of secondary hair follicles and the secondary hair follicles/primary hair follicles ratio increased from 85 to 105 gestational days. Bioinformatic analysis identified 238, 35, and 348 differentially expressed proteins in the respective comparison periods. Focal adhesion, ECM-receptor interaction, and the estrogen signaling pathway all played important roles in secondary hair follicle development. COL1A1, THBS3, ITGA6, COL6A1, and THBS4 were identified as potential candidate proteins in the initiation of secondary hair follicles. This study provides valuable proteomics data on secondary hair follicle development and thus has deepened our understanding of the molecular mechanisms underlying wool quality traits in fine-wool sheep.https://doi.org/10.1371/journal.pone.0315637 |
| spellingShingle | Li-Xia Qiu Qian Yu Hua-Qian Zhou Wen-Hua Fan Jing-Jing Zheng Yong-Lin Yang Wen-Zhe Zhang Xin Cao Hua Yang TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep. PLoS ONE |
| title | TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep. |
| title_full | TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep. |
| title_fullStr | TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep. |
| title_full_unstemmed | TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep. |
| title_short | TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep. |
| title_sort | tmt based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine wool sheep |
| url | https://doi.org/10.1371/journal.pone.0315637 |
| work_keys_str_mv | AT lixiaqiu tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT qianyu tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT huaqianzhou tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT wenhuafan tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT jingjingzheng tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT yonglinyang tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT wenzhezhang tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT xincao tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep AT huayang tmtbasedquantitativeproteomicsrevealsthegeneticmechanismsofsecondaryhairfollicledevelopmentinfinewoolsheep |