Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering
This study investigated the role of the <i>Zymomonas mobilis recA</i> gene in conferring stress resistance when expressed in <i>Escherichia coli</i>. The <i>recA</i> gene was cloned and expressed in <i>E. coli</i> BL21(DE3), producing a 39 kDa polypept...
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2024-12-01
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| Series: | Fermentation |
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| author | Yupaporn Phannarangsee Haruthairat Kitwetcharoen Sudarat Thanonkeo Preekamol Klanrit Mamoru Yamada Pornthap Thanonkeo |
| author_facet | Yupaporn Phannarangsee Haruthairat Kitwetcharoen Sudarat Thanonkeo Preekamol Klanrit Mamoru Yamada Pornthap Thanonkeo |
| author_sort | Yupaporn Phannarangsee |
| collection | DOAJ |
| description | This study investigated the role of the <i>Zymomonas mobilis recA</i> gene in conferring stress resistance when expressed in <i>Escherichia coli</i>. The <i>recA</i> gene was cloned and expressed in <i>E. coli</i> BL21(DE3), producing a 39 kDa polypeptide. The results of comparative analyses demonstrated that the recombinant strain significantly enhanced survival rates under various stress conditions. In oxidative stress tests, the recombinant <i>E. coli</i> pET-22b(+)-recA exhibited superior survival at 3 mM and 5 mM H<sub>2</sub>O<sub>2</sub> concentrations. Heat stress experiments at 50 °C and 55 °C revealed increased survival for the recombinant strain. Under ethanol stress, particularly at 20% (<i>v</i>/<i>v</i>), <i>E. coli</i> pET-22b(+)-recA displayed higher viability than controls. UV-C exposure tests further highlighted the protective effect of <i>recA</i> expression, with the recombinant strain maintaining viability after 60 min of exposure, while control strains showed no survival. These results indicate that the <i>Z. mobilis recA</i> gene product enhances resistance to oxidative, heat, ethanol, and UV-C stresses when expressed in <i>E. coli</i>. This study elucidates the broad stress-protective functions of the RecA protein across bacterial species and suggests potential applications in developing stress-tolerant bacterial strains for biotechnological purposes. |
| format | Article |
| id | doaj-art-184db2f5a4274ca48b5e682b6e064564 |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Fermentation |
| spelling | doaj-art-184db2f5a4274ca48b5e682b6e0645642024-12-27T14:25:25ZengMDPI AGFermentation2311-56372024-12-01101261710.3390/fermentation10120617Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain EngineeringYupaporn Phannarangsee0Haruthairat Kitwetcharoen1Sudarat Thanonkeo2Preekamol Klanrit3Mamoru Yamada4Pornthap Thanonkeo5Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandWalai Rukhavej Botanical Research Institute and Center of Excellence in Biodiversity Research, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, JapanDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandThis study investigated the role of the <i>Zymomonas mobilis recA</i> gene in conferring stress resistance when expressed in <i>Escherichia coli</i>. The <i>recA</i> gene was cloned and expressed in <i>E. coli</i> BL21(DE3), producing a 39 kDa polypeptide. The results of comparative analyses demonstrated that the recombinant strain significantly enhanced survival rates under various stress conditions. In oxidative stress tests, the recombinant <i>E. coli</i> pET-22b(+)-recA exhibited superior survival at 3 mM and 5 mM H<sub>2</sub>O<sub>2</sub> concentrations. Heat stress experiments at 50 °C and 55 °C revealed increased survival for the recombinant strain. Under ethanol stress, particularly at 20% (<i>v</i>/<i>v</i>), <i>E. coli</i> pET-22b(+)-recA displayed higher viability than controls. UV-C exposure tests further highlighted the protective effect of <i>recA</i> expression, with the recombinant strain maintaining viability after 60 min of exposure, while control strains showed no survival. These results indicate that the <i>Z. mobilis recA</i> gene product enhances resistance to oxidative, heat, ethanol, and UV-C stresses when expressed in <i>E. coli</i>. This study elucidates the broad stress-protective functions of the RecA protein across bacterial species and suggests potential applications in developing stress-tolerant bacterial strains for biotechnological purposes.https://www.mdpi.com/2311-5637/10/12/617DNA repairethanol stressgene expressionheat stressoxidative stressSOS response |
| spellingShingle | Yupaporn Phannarangsee Haruthairat Kitwetcharoen Sudarat Thanonkeo Preekamol Klanrit Mamoru Yamada Pornthap Thanonkeo Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering Fermentation DNA repair ethanol stress gene expression heat stress oxidative stress SOS response |
| title | Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering |
| title_full | Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering |
| title_fullStr | Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering |
| title_full_unstemmed | Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering |
| title_short | Enhanced Multi-Stress Tolerance in <i>Escherichia coli</i> via the Heterologous Expression of <i>Zymomonas mobilis recA</i>: Implications for Industrial Strain Engineering |
| title_sort | enhanced multi stress tolerance in i escherichia coli i via the heterologous expression of i zymomonas mobilis reca i implications for industrial strain engineering |
| topic | DNA repair ethanol stress gene expression heat stress oxidative stress SOS response |
| url | https://www.mdpi.com/2311-5637/10/12/617 |
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