Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa
IntroductionMicroorganisms play an important role in the embryonic development of plant seeds; however, there are no existing reports on the microbial communities associated with Fritillaria cirrhosa before and after embryo after-ripening.MethodsIn this study, the microbial communities of Fritillari...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1544052/full |
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| author | Wenshang Li Wenshang Li Can Zhao Can Zhao Qian Tao Qian Tao Weimin Zhang Weimin Zhang Hai Wang Guiqi Han Guiqi Han Zhuyun Yan Zhuyun Yan |
| author_facet | Wenshang Li Wenshang Li Can Zhao Can Zhao Qian Tao Qian Tao Weimin Zhang Weimin Zhang Hai Wang Guiqi Han Guiqi Han Zhuyun Yan Zhuyun Yan |
| author_sort | Wenshang Li |
| collection | DOAJ |
| description | IntroductionMicroorganisms play an important role in the embryonic development of plant seeds; however, there are no existing reports on the microbial communities associated with Fritillaria cirrhosa before and after embryo after-ripening.MethodsIn this study, the microbial communities of Fritillaria cirrhosa seeds before and after after-ripening were analyzed using the Illumina MiSeq platform, targeting the V4–V5 region of the bacterial 16S rRNA gene and the ITS1 and ITS2 regions of fungal ribosomal RNA.ResultsThe results showed that bacterial communities were more susceptible to environmental stress and exhibited greater fluctuations compared to fungal communities, as reflected in higher diversity and significant changes in the relative abundance of dominant genera and species. After embryo after-ripening, the dominant fungal genera were Botrytis (SBAR, 29.35%), Tetracladium (SBAR, 15.86%), Ilyonectria (SBAR, 15.35%), and Mrakia (SBAR, 13.14%), while the dominant bacterial genera were Pseudomonas (SBAR, 26.69%) and Stenotrophomonas (SBAR, 16.30%).Prediction results suggested that the bacterial communities with sharply increased relative abundance after embryo after-ripening may interact with seeds through various pathways, including carbohydrate metabolism, absorption and utilization of nitrogen (N), sulfur (S), phosphorus (P), and iron (Fe), as well as secretion of antibiotics, vitamins, cytokinins, and amino acids. Functional validation revealed that most culturable fungi with sharply increased relative abundance had cellulase-degrading abilities, while most of the bacterial isolates were capable of absorbing and utilizing C, N, S, P, and Fe elements. Microbial co-occurrence network analysis indicated that the microbiome after embryo after-ripening formed an unstable, expansive, and rapidly changing network.DiscussionIn summary, this study revealed the overall dynamics of the microbiome in Fritillaria cirrhosa seeds after embryo after-ripening and identified key microbial taxa exhibiting sharp shifts in relative abundance. This work provides a foundational understanding of the microbial succession associated with seed embryo after-ripening in Fritillaria cirrhosa, which may support seed after-ripening and germination, and enhance seed stress resistance. |
| format | Article |
| id | doaj-art-184b26668d604ac38e8970c0e91d8036 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-184b26668d604ac38e8970c0e91d80362025-08-20T02:15:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15440521544052Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosaWenshang Li0Wenshang Li1Can Zhao2Can Zhao3Qian Tao4Qian Tao5Weimin Zhang6Weimin Zhang7Hai Wang8Guiqi Han9Guiqi Han10Zhuyun Yan11Zhuyun Yan12State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaIntroductionMicroorganisms play an important role in the embryonic development of plant seeds; however, there are no existing reports on the microbial communities associated with Fritillaria cirrhosa before and after embryo after-ripening.MethodsIn this study, the microbial communities of Fritillaria cirrhosa seeds before and after after-ripening were analyzed using the Illumina MiSeq platform, targeting the V4–V5 region of the bacterial 16S rRNA gene and the ITS1 and ITS2 regions of fungal ribosomal RNA.ResultsThe results showed that bacterial communities were more susceptible to environmental stress and exhibited greater fluctuations compared to fungal communities, as reflected in higher diversity and significant changes in the relative abundance of dominant genera and species. After embryo after-ripening, the dominant fungal genera were Botrytis (SBAR, 29.35%), Tetracladium (SBAR, 15.86%), Ilyonectria (SBAR, 15.35%), and Mrakia (SBAR, 13.14%), while the dominant bacterial genera were Pseudomonas (SBAR, 26.69%) and Stenotrophomonas (SBAR, 16.30%).Prediction results suggested that the bacterial communities with sharply increased relative abundance after embryo after-ripening may interact with seeds through various pathways, including carbohydrate metabolism, absorption and utilization of nitrogen (N), sulfur (S), phosphorus (P), and iron (Fe), as well as secretion of antibiotics, vitamins, cytokinins, and amino acids. Functional validation revealed that most culturable fungi with sharply increased relative abundance had cellulase-degrading abilities, while most of the bacterial isolates were capable of absorbing and utilizing C, N, S, P, and Fe elements. Microbial co-occurrence network analysis indicated that the microbiome after embryo after-ripening formed an unstable, expansive, and rapidly changing network.DiscussionIn summary, this study revealed the overall dynamics of the microbiome in Fritillaria cirrhosa seeds after embryo after-ripening and identified key microbial taxa exhibiting sharp shifts in relative abundance. This work provides a foundational understanding of the microbial succession associated with seed embryo after-ripening in Fritillaria cirrhosa, which may support seed after-ripening and germination, and enhance seed stress resistance.https://www.frontiersin.org/articles/10.3389/fpls.2025.1544052/fullFritillaria cirrhosaseed embryo after-ripeningmicrobiomeseed germinationbacterial communitiesfungal communities |
| spellingShingle | Wenshang Li Wenshang Li Can Zhao Can Zhao Qian Tao Qian Tao Weimin Zhang Weimin Zhang Hai Wang Guiqi Han Guiqi Han Zhuyun Yan Zhuyun Yan Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa Frontiers in Plant Science Fritillaria cirrhosa seed embryo after-ripening microbiome seed germination bacterial communities fungal communities |
| title | Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa |
| title_full | Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa |
| title_fullStr | Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa |
| title_full_unstemmed | Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa |
| title_short | Study on the changes in the microbiome before and after seed embryo after-ripening of Fritillaria cirrhosa |
| title_sort | study on the changes in the microbiome before and after seed embryo after ripening of fritillaria cirrhosa |
| topic | Fritillaria cirrhosa seed embryo after-ripening microbiome seed germination bacterial communities fungal communities |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1544052/full |
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