Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies
Abstract African swine fever virus (ASFV), the causative agent of African swine fever, is a fatal haemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs); however, PAM isolation and maintenance are technically cha...
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| Format: | Article |
| Language: | English |
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BMC
2025-06-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-025-01543-7 |
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| author | Eun-Gyeong Lee Sang-Min Kang Dongseob Tark |
| author_facet | Eun-Gyeong Lee Sang-Min Kang Dongseob Tark |
| author_sort | Eun-Gyeong Lee |
| collection | DOAJ |
| description | Abstract African swine fever virus (ASFV), the causative agent of African swine fever, is a fatal haemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs); however, PAM isolation and maintenance are technically challenging, and genetic manipulation of these cells is difficult. The lack of suitable cell lines that support ASFV infection and replication has significantly hindered vaccine development. This study aimed to overcome these limitations and advance ASFV research. The results demonstrate that the foetal porcine kidney cells (LFBK) are suitable for ASFV studies. We observed that ASFV replicated significantly more efficiently in LFBK cells than in PAMs. Furthermore, LFBK cells exhibited antiviral immune responses similar to PAMs following ASFV infection or DNA analog. These findings suggest that the LFBK cell line could provide a much-needed platform for studying ASFV replication and pathogenesis while serving as a valuable tool for understanding ASFV-induced immune mechanisms. |
| format | Article |
| id | doaj-art-18433bb375154184a539a22444ec0ee6 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-18433bb375154184a539a22444ec0ee62025-08-20T03:43:15ZengBMCVeterinary Research1297-97162025-06-0156111110.1186/s13567-025-01543-7Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studiesEun-Gyeong Lee0Sang-Min Kang1Dongseob Tark2Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National UniversityLaboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National UniversityLaboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National UniversityAbstract African swine fever virus (ASFV), the causative agent of African swine fever, is a fatal haemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs); however, PAM isolation and maintenance are technically challenging, and genetic manipulation of these cells is difficult. The lack of suitable cell lines that support ASFV infection and replication has significantly hindered vaccine development. This study aimed to overcome these limitations and advance ASFV research. The results demonstrate that the foetal porcine kidney cells (LFBK) are suitable for ASFV studies. We observed that ASFV replicated significantly more efficiently in LFBK cells than in PAMs. Furthermore, LFBK cells exhibited antiviral immune responses similar to PAMs following ASFV infection or DNA analog. These findings suggest that the LFBK cell line could provide a much-needed platform for studying ASFV replication and pathogenesis while serving as a valuable tool for understanding ASFV-induced immune mechanisms.https://doi.org/10.1186/s13567-025-01543-7ASFVLFBKPAMsType I IFNsuitable cell line |
| spellingShingle | Eun-Gyeong Lee Sang-Min Kang Dongseob Tark Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies Veterinary Research ASFV LFBK PAMs Type I IFN suitable cell line |
| title | Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies |
| title_full | Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies |
| title_fullStr | Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies |
| title_full_unstemmed | Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies |
| title_short | Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies |
| title_sort | highly suitable lfbk cells for african swine fever virus replication and type i interferon induced immune studies |
| topic | ASFV LFBK PAMs Type I IFN suitable cell line |
| url | https://doi.org/10.1186/s13567-025-01543-7 |
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