The great escape: Pseudomonas breaks out of the lung
The Gram-negative bacterium Pseudomonas aeruginosa is a major cause of hospital-acquired infections and the focus of much attention due to its resistance to many conventional antibiotics. It harbors a wide range of disease-promoting virulence factors, including a type III secretion...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2015-09-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/the-great-escape-pseudomonas-breaks-out-of-the-lung/ |
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| author | Angelica Zhang Stephanie M. Rangel Alan R. Hauser |
| author_facet | Angelica Zhang Stephanie M. Rangel Alan R. Hauser |
| author_sort | Angelica Zhang |
| collection | DOAJ |
| description | The Gram-negative bacterium Pseudomonas aeruginosa is a major cause of hospital-acquired infections and the focus of much attention due to its resistance to many conventional antibiotics. It harbors a wide range of disease-promoting virulence factors, including a type III secretion system. Here we review our recent study of ExoS, one of the effector proteins exported by this type III secretion system. Using a mouse model of pneumonia, we showed that the ADP-ribosyltransferase (ADPRT) activity of ExoS caused formation of “fields of cell injection” (FOCI) in the lungs. These FOCI represented ExoS-injected clusters of type I pneumocytes that became compromised, leading to disruption of the pulmonary-vascular barrier and subsequent bacterial dissemination from the lungs to the bloodstream. We discuss the potential mechanisms by which these processes occur as well as the novel techniques used to study ExoS function in vivo. |
| format | Article |
| id | doaj-art-183c290d6562455d8efa69b058b2d5b4 |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-183c290d6562455d8efa69b058b2d5b42025-08-20T02:53:21ZengShared Science Publishers OGMicrobial Cell2311-26382015-09-0121040941110.15698/mic2015.10.234123455678The great escape: Pseudomonas breaks out of the lungAngelica Zhang0Stephanie M. Rangel1Alan R. Hauser2Department of Microbiology/Immunology, Northwestern University, Feinberg School of Medicine, Chicago Illinois, USA.Department of Microbiology/Immunology, Northwestern University, Feinberg School of Medicine, Chicago Illinois, USA.Department of Microbiology/Immunology, Northwestern University, Feinberg School of Medicine, Chicago Illinois, USA.The Gram-negative bacterium Pseudomonas aeruginosa is a major cause of hospital-acquired infections and the focus of much attention due to its resistance to many conventional antibiotics. It harbors a wide range of disease-promoting virulence factors, including a type III secretion system. Here we review our recent study of ExoS, one of the effector proteins exported by this type III secretion system. Using a mouse model of pneumonia, we showed that the ADP-ribosyltransferase (ADPRT) activity of ExoS caused formation of “fields of cell injection” (FOCI) in the lungs. These FOCI represented ExoS-injected clusters of type I pneumocytes that became compromised, leading to disruption of the pulmonary-vascular barrier and subsequent bacterial dissemination from the lungs to the bloodstream. We discuss the potential mechanisms by which these processes occur as well as the novel techniques used to study ExoS function in vivo.http://microbialcell.com/researcharticles/the-great-escape-pseudomonas-breaks-out-of-the-lung/Pseudomonas aeruginosaExoSpneumoniadissemination |
| spellingShingle | Angelica Zhang Stephanie M. Rangel Alan R. Hauser The great escape: Pseudomonas breaks out of the lung Microbial Cell Pseudomonas aeruginosa ExoS pneumonia dissemination |
| title | The great escape: Pseudomonas breaks out of the lung |
| title_full | The great escape: Pseudomonas breaks out of the lung |
| title_fullStr | The great escape: Pseudomonas breaks out of the lung |
| title_full_unstemmed | The great escape: Pseudomonas breaks out of the lung |
| title_short | The great escape: Pseudomonas breaks out of the lung |
| title_sort | great escape pseudomonas breaks out of the lung |
| topic | Pseudomonas aeruginosa ExoS pneumonia dissemination |
| url | http://microbialcell.com/researcharticles/the-great-escape-pseudomonas-breaks-out-of-the-lung/ |
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