Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies
ABSTRACT Recent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision. This review focuses on two powerful methodologies: deep mutational scanning and CRISPR-based genome editing, that enable comprehensiv...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2025-05-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00508-24 |
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| _version_ | 1850257140064714752 |
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| author | Mercedes Paz Gonzalo Moratorio |
| author_facet | Mercedes Paz Gonzalo Moratorio |
| author_sort | Mercedes Paz |
| collection | DOAJ |
| description | ABSTRACT Recent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision. This review focuses on two powerful methodologies: deep mutational scanning and CRISPR-based genome editing, that enable comprehensive mutagenesis and detailed functional characterization of viral proteins. These approaches have significantly deepened our understanding of the molecular determinants driving viral evolution and adaptation. Furthermore, we discuss how these advances provide transformative insights for future vaccine development and therapeutic strategies. |
| format | Article |
| id | doaj-art-18379f5d3e5d442eb9070bfdd93b1302 |
| institution | OA Journals |
| issn | 2379-5042 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj-art-18379f5d3e5d442eb9070bfdd93b13022025-08-20T01:56:29ZengAmerican Society for MicrobiologymSphere2379-50422025-05-0110510.1128/msphere.00508-24Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studiesMercedes Paz0Gonzalo Moratorio1Laboratory of Experimental Virus Evolution, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratory of Experimental Virus Evolution, Institut Pasteur de Montevideo, Montevideo, UruguayABSTRACT Recent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision. This review focuses on two powerful methodologies: deep mutational scanning and CRISPR-based genome editing, that enable comprehensive mutagenesis and detailed functional characterization of viral proteins. These approaches have significantly deepened our understanding of the molecular determinants driving viral evolution and adaptation. Furthermore, we discuss how these advances provide transformative insights for future vaccine development and therapeutic strategies.https://journals.asm.org/doi/10.1128/msphere.00508-24deep mutational scanningCRISPR-engineered virusesvirus evolution |
| spellingShingle | Mercedes Paz Gonzalo Moratorio Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies mSphere deep mutational scanning CRISPR-engineered viruses virus evolution |
| title | Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies |
| title_full | Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies |
| title_fullStr | Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies |
| title_full_unstemmed | Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies |
| title_short | Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies |
| title_sort | deep mutational scanning and crispr engineered viruses tools for evolutionary and functional genomics studies |
| topic | deep mutational scanning CRISPR-engineered viruses virus evolution |
| url | https://journals.asm.org/doi/10.1128/msphere.00508-24 |
| work_keys_str_mv | AT mercedespaz deepmutationalscanningandcrisprengineeredvirusestoolsforevolutionaryandfunctionalgenomicsstudies AT gonzalomoratorio deepmutationalscanningandcrisprengineeredvirusestoolsforevolutionaryandfunctionalgenomicsstudies |