Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications
Polyrhodanines have been broadly utilized in diverse fields due to their attractive features. The effect of polyrhodanine- (PR-) based materials on human cells can be considered a controversial matter, while many contradictions exist. In this study, we focused on the synthesis of polyrhodanine/Fe3O4...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2021/9972664 |
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| author | Seyyed Mojtaba Mousavi Seyyed Alireza Hashemi Ahmad Gholami Navid Omidifar Maryam Zarei Sonia Bahrani Khadije Yousefi Wei-Hung Chiang Aziz Babapoor |
| author_facet | Seyyed Mojtaba Mousavi Seyyed Alireza Hashemi Ahmad Gholami Navid Omidifar Maryam Zarei Sonia Bahrani Khadije Yousefi Wei-Hung Chiang Aziz Babapoor |
| author_sort | Seyyed Mojtaba Mousavi |
| collection | DOAJ |
| description | Polyrhodanines have been broadly utilized in diverse fields due to their attractive features. The effect of polyrhodanine- (PR-) based materials on human cells can be considered a controversial matter, while many contradictions exist. In this study, we focused on the synthesis of polyrhodanine/Fe3O4 modified by graphene oxide and the effect of kombucha (Ko) supernatant on results. The general structure of synthetic compounds was determined in detail through Fourier-transform infrared spectroscopy (FT-IR). Also, obtained compounds were morphologically, magnetically, and chemically characterized using scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM), energy dispersive X-ray (EDX) analysis. The antibacterial effects of all synthesized nanomaterials were done according to CLSI against four infamous pathogens. Also, the cytotoxic effects of the synthesized compounds on the human liver cancer cell line (Hep-G2) were assessed by MTT assay. Our results showed that Go/Fe has the highest average inhibitory effect against Escherichia coli and Pseudomonas aeruginosa, and this compound possesses the least antimicrobial effect on Staphylococcus aureus. Considering the viability percent of cells in the PR/GO/Fe3O4 compound and comparing it with GO/Fe3O4, it can be understood that the toxic effects of polyrhodanine can diminish the metabolic activity of cells at higher concentrations (mostly more than 50 µg/mL), and PR/Fe3O4/Ko exhibited some promotive effects on cell growth, which enhanced the viability percent to more than 100%. Similarly, the cell viability percent of PR/GO/Fe3O4/KO compared to PR/GO/Fe3O4 is much higher, which can be attributed to the presence of kombucha in the compound. Consequently, based on the results, it can be concluded that this novel polyrhodanine-based nanocompound can act as drug carriers due to their low toxic effects and may open a new window on the antibacterial agents. |
| format | Article |
| id | doaj-art-1830ccfae8e5471497b8f7c18e39cbfd |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-1830ccfae8e5471497b8f7c18e39cbfd2025-02-03T01:27:24ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2021-01-01202110.1155/2021/99726649972664Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial ApplicationsSeyyed Mojtaba Mousavi0Seyyed Alireza Hashemi1Ahmad Gholami2Navid Omidifar3Maryam Zarei4Sonia Bahrani5Khadije Yousefi6Wei-Hung Chiang7Aziz Babapoor8Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanNanomaterials and Polymer Nanocomposites Laboratory, School of Engineering, University of British Columbia, Kelowna, BC V1V 1V7, CanadaPharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBiotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, IranBiotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, IranBiotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanDepartment of Chemical Engineering, University of Mohaghegh Ardabili (UMA), Ardabil, IranPolyrhodanines have been broadly utilized in diverse fields due to their attractive features. The effect of polyrhodanine- (PR-) based materials on human cells can be considered a controversial matter, while many contradictions exist. In this study, we focused on the synthesis of polyrhodanine/Fe3O4 modified by graphene oxide and the effect of kombucha (Ko) supernatant on results. The general structure of synthetic compounds was determined in detail through Fourier-transform infrared spectroscopy (FT-IR). Also, obtained compounds were morphologically, magnetically, and chemically characterized using scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM), energy dispersive X-ray (EDX) analysis. The antibacterial effects of all synthesized nanomaterials were done according to CLSI against four infamous pathogens. Also, the cytotoxic effects of the synthesized compounds on the human liver cancer cell line (Hep-G2) were assessed by MTT assay. Our results showed that Go/Fe has the highest average inhibitory effect against Escherichia coli and Pseudomonas aeruginosa, and this compound possesses the least antimicrobial effect on Staphylococcus aureus. Considering the viability percent of cells in the PR/GO/Fe3O4 compound and comparing it with GO/Fe3O4, it can be understood that the toxic effects of polyrhodanine can diminish the metabolic activity of cells at higher concentrations (mostly more than 50 µg/mL), and PR/Fe3O4/Ko exhibited some promotive effects on cell growth, which enhanced the viability percent to more than 100%. Similarly, the cell viability percent of PR/GO/Fe3O4/KO compared to PR/GO/Fe3O4 is much higher, which can be attributed to the presence of kombucha in the compound. Consequently, based on the results, it can be concluded that this novel polyrhodanine-based nanocompound can act as drug carriers due to their low toxic effects and may open a new window on the antibacterial agents.http://dx.doi.org/10.1155/2021/9972664 |
| spellingShingle | Seyyed Mojtaba Mousavi Seyyed Alireza Hashemi Ahmad Gholami Navid Omidifar Maryam Zarei Sonia Bahrani Khadije Yousefi Wei-Hung Chiang Aziz Babapoor Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications Bioinorganic Chemistry and Applications |
| title | Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications |
| title_full | Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications |
| title_fullStr | Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications |
| title_full_unstemmed | Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications |
| title_short | Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications |
| title_sort | bioinorganic synthesis of polyrhodanine stabilized fe3o4 graphene oxide in microbial supernatant media for anticancer and antibacterial applications |
| url | http://dx.doi.org/10.1155/2021/9972664 |
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