RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway

Accumulating evidence indicates that ER-phagy serves as a key adaptive regulatory mechanism in response to various stress conditions. However, the exact mechanisms underlying ER-phagy in the pathogenesis of intervertebral disc degeneration remain largely unclear. In the present study, we demonstrate...

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Main Authors: Luo Rongjin, Liang Huaizhen, Zhang Weifeng, Li Gaocai, Zhao Kangcheng, Hua Wenbin, Song Yu, Yang Cao
Format: Article
Language:English
Published: China Science Publishing & Media Ltd. 2022-03-01
Series:Acta Biochimica et Biophysica Sinica
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Online Access:https://www.sciengine.com/doi/10.3724/abbs.2022024
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author Luo Rongjin
Liang Huaizhen
Zhang Weifeng
Li Gaocai
Zhao Kangcheng
Hua Wenbin
Song Yu
Yang Cao
author_facet Luo Rongjin
Liang Huaizhen
Zhang Weifeng
Li Gaocai
Zhao Kangcheng
Hua Wenbin
Song Yu
Yang Cao
author_sort Luo Rongjin
collection DOAJ
description Accumulating evidence indicates that ER-phagy serves as a key adaptive regulatory mechanism in response to various stress conditions. However, the exact mechanisms underlying ER-phagy in the pathogenesis of intervertebral disc degeneration remain largely unclear. In the present study, we demonstrated that RETREG1-mediated ER-phagy is induced by glucose deprivation (GD) treatment, along with ER stress activation and cell function decline. Importantly, ER-phagy was shown to be crucial for cell survival under GD conditions. Furthermore, ER stress was suggested as an upstream event of ER-phagy upon GD treatment and upregulation of ER-phagy could counteract the ER stress response. Therefore, our findings indicate that RETREG1-mediated ER-phagy activation protects against GD treatment-induced cell injury via modulating ER stress in human nucleus pulposus cells.
format Article
id doaj-art-182faffad82943a2bd1339a3ebcaeb24
institution OA Journals
issn 1672-9145
language English
publishDate 2022-03-01
publisher China Science Publishing & Media Ltd.
record_format Article
series Acta Biochimica et Biophysica Sinica
spelling doaj-art-182faffad82943a2bd1339a3ebcaeb242025-08-20T01:52:15ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-03-015452453610.3724/abbs.202202420d259ccRETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathwayLuo Rongjin0Liang Huaizhen1Zhang Weifeng2Li Gaocai3Zhao Kangcheng4Hua Wenbin5Song Yu6Yang Cao7[][][][][][][][]Accumulating evidence indicates that ER-phagy serves as a key adaptive regulatory mechanism in response to various stress conditions. However, the exact mechanisms underlying ER-phagy in the pathogenesis of intervertebral disc degeneration remain largely unclear. In the present study, we demonstrated that RETREG1-mediated ER-phagy is induced by glucose deprivation (GD) treatment, along with ER stress activation and cell function decline. Importantly, ER-phagy was shown to be crucial for cell survival under GD conditions. Furthermore, ER stress was suggested as an upstream event of ER-phagy upon GD treatment and upregulation of ER-phagy could counteract the ER stress response. Therefore, our findings indicate that RETREG1-mediated ER-phagy activation protects against GD treatment-induced cell injury via modulating ER stress in human nucleus pulposus cells.https://www.sciengine.com/doi/10.3724/abbs.2022024intervertebral disc degenerationsenescenceapoptosisER-phagyER stress
spellingShingle Luo Rongjin
Liang Huaizhen
Zhang Weifeng
Li Gaocai
Zhao Kangcheng
Hua Wenbin
Song Yu
Yang Cao
RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway
Acta Biochimica et Biophysica Sinica
intervertebral disc degeneration
senescence
apoptosis
ER-phagy
ER stress
title RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway
title_full RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway
title_fullStr RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway
title_full_unstemmed RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway
title_short RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway
title_sort retreg1 mediated er phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via er stress pathway
topic intervertebral disc degeneration
senescence
apoptosis
ER-phagy
ER stress
url https://www.sciengine.com/doi/10.3724/abbs.2022024
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