Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.

The prospects of using produced water (PW), a by-product of oil extraction, as a cultivation medium to not only grow microalgae but also generate value-added by-products has not been much investigated. This study demonstrates the ability of a newly isolated microalga, Asterarcys sp. RA100, for growt...

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Main Authors: Raeid M M Abed, Huda Al Battashi, Thirumahal Muthukrishnan, Hamzah Al-Bartamani, Mahmood Al-Hinai, Maryam Al-Baluchi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0325759
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author Raeid M M Abed
Huda Al Battashi
Thirumahal Muthukrishnan
Hamzah Al-Bartamani
Mahmood Al-Hinai
Maryam Al-Baluchi
author_facet Raeid M M Abed
Huda Al Battashi
Thirumahal Muthukrishnan
Hamzah Al-Bartamani
Mahmood Al-Hinai
Maryam Al-Baluchi
author_sort Raeid M M Abed
collection DOAJ
description The prospects of using produced water (PW), a by-product of oil extraction, as a cultivation medium to not only grow microalgae but also generate value-added by-products has not been much investigated. This study demonstrates the ability of a newly isolated microalga, Asterarcys sp. RA100, for growth in PW and biodiesel production. Although the used PW was slightly alkaline (pH < 10), nutrient deficient and high in boron content, Asterarcys sp. RA100 exhibited good growth with phosphate, and nitrate, reaching optimal growth at 1% salinity, 25°C, 150 rpm, and 4000-8000 Lux LED light intensity. To test for its scalability in a greenhouse, Asterarcys sp. RA100 exhibited areal productivity of 10.3 ± 0.5 g m-2 day-1. Lipid accumulation in Asterarcys sp. RA100 reached 27.0 ± 5.1% of dry weight when grown in PW. The resulting fatty acids methyl esters (FAME) displayed properties aligning with international biodiesel standards. The FAME profiles showed elevated contents of palmitic acid (C16:0), elaidic acid (C18:1n9t), stearic acid (C18:0) and palmitoleic acid (C16:1n7C). This study demonstrates the immense potential of Asterarcys sp. RA100 to grow in PW and to serve as valuable feedstock for biodiesel production thereby, providing an eco-friendly method to re-use PW and sustain future energy demands.
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spelling doaj-art-180ecc128bf04026a1107e9812f867d52025-08-20T02:10:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032575910.1371/journal.pone.0325759Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.Raeid M M AbedHuda Al BattashiThirumahal MuthukrishnanHamzah Al-BartamaniMahmood Al-HinaiMaryam Al-BaluchiThe prospects of using produced water (PW), a by-product of oil extraction, as a cultivation medium to not only grow microalgae but also generate value-added by-products has not been much investigated. This study demonstrates the ability of a newly isolated microalga, Asterarcys sp. RA100, for growth in PW and biodiesel production. Although the used PW was slightly alkaline (pH < 10), nutrient deficient and high in boron content, Asterarcys sp. RA100 exhibited good growth with phosphate, and nitrate, reaching optimal growth at 1% salinity, 25°C, 150 rpm, and 4000-8000 Lux LED light intensity. To test for its scalability in a greenhouse, Asterarcys sp. RA100 exhibited areal productivity of 10.3 ± 0.5 g m-2 day-1. Lipid accumulation in Asterarcys sp. RA100 reached 27.0 ± 5.1% of dry weight when grown in PW. The resulting fatty acids methyl esters (FAME) displayed properties aligning with international biodiesel standards. The FAME profiles showed elevated contents of palmitic acid (C16:0), elaidic acid (C18:1n9t), stearic acid (C18:0) and palmitoleic acid (C16:1n7C). This study demonstrates the immense potential of Asterarcys sp. RA100 to grow in PW and to serve as valuable feedstock for biodiesel production thereby, providing an eco-friendly method to re-use PW and sustain future energy demands.https://doi.org/10.1371/journal.pone.0325759
spellingShingle Raeid M M Abed
Huda Al Battashi
Thirumahal Muthukrishnan
Hamzah Al-Bartamani
Mahmood Al-Hinai
Maryam Al-Baluchi
Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.
PLoS ONE
title Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.
title_full Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.
title_fullStr Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.
title_full_unstemmed Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.
title_short Growth of a newly isolated oleaginous microalgal strain (Asterarcys sp. RA100) in oil produced water and its potential for biodiesel production.
title_sort growth of a newly isolated oleaginous microalgal strain asterarcys sp ra100 in oil produced water and its potential for biodiesel production
url https://doi.org/10.1371/journal.pone.0325759
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