Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea
<p>Effective extraction of anticancer and antioxidant principles from Turkish green tea were main purpose of this work. The pre-optimized experimental condition for liquid extraction was employed for comparative appraisal. Not only extraction methods also nature of the green tea samples (fres...
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| Language: | English |
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izzet kara
2015-07-01
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| Series: | International Journal of Secondary Metabolite |
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| Online Access: | http://ijate.net/index.php/ijsm/article/view/82 |
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| author | Ezgi DEMİR Gönül SERDAR Münevver SÖKMEN |
| author_facet | Ezgi DEMİR Gönül SERDAR Münevver SÖKMEN |
| author_sort | Ezgi DEMİR |
| collection | DOAJ |
| description | <p>Effective extraction of anticancer and antioxidant principles from Turkish green tea were main purpose of this work. The pre-optimized experimental condition for liquid extraction was employed for comparative appraisal. Not only extraction methods also nature of the green tea samples (fresh, dried or frozen) and quantitative yields related to collection periods were investigated. After extraction of the green tea with various techniques the extract was partitioned with chloroform to remove caffeine, after that the extract was partitioned with ethyl acetate to obtain catechin mixture. Quantification of individual catechins was carried out by HPLC and analysis results proved that epigallocatechin gallate (EGCG) was main catechin specie present in all extracts. The results indicate that hot water extraction (at 80 <sup>0</sup>C) provides higher catechin yield when compared to other methods. The highest extract yields were obtained with dried leaves collected in second collection period. The crude catechin mixture contains high amount of EGCG and might be used as raw material for production of plant remedies at industrial scale.</p> |
| format | Article |
| id | doaj-art-180bde65d697430e8efbae580507e5bc |
| institution | OA Journals |
| issn | 2148-6905 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | izzet kara |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj-art-180bde65d697430e8efbae580507e5bc2025-08-20T02:13:01Zengizzet karaInternational Journal of Secondary Metabolite2148-69052015-07-012261Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green TeaEzgi DEMİR0Gönül SERDAR1Münevver SÖKMEN2Karadeniz Technical UniversityKaradeniz Technical UniversityKaradeniz Technical University<p>Effective extraction of anticancer and antioxidant principles from Turkish green tea were main purpose of this work. The pre-optimized experimental condition for liquid extraction was employed for comparative appraisal. Not only extraction methods also nature of the green tea samples (fresh, dried or frozen) and quantitative yields related to collection periods were investigated. After extraction of the green tea with various techniques the extract was partitioned with chloroform to remove caffeine, after that the extract was partitioned with ethyl acetate to obtain catechin mixture. Quantification of individual catechins was carried out by HPLC and analysis results proved that epigallocatechin gallate (EGCG) was main catechin specie present in all extracts. The results indicate that hot water extraction (at 80 <sup>0</sup>C) provides higher catechin yield when compared to other methods. The highest extract yields were obtained with dried leaves collected in second collection period. The crude catechin mixture contains high amount of EGCG and might be used as raw material for production of plant remedies at industrial scale.</p>http://ijate.net/index.php/ijsm/article/view/82Turkish green teaEGCGextractionHPLCanticancer |
| spellingShingle | Ezgi DEMİR Gönül SERDAR Münevver SÖKMEN Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea International Journal of Secondary Metabolite Turkish green tea EGCG extraction HPLC anticancer |
| title | Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea |
| title_full | Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea |
| title_fullStr | Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea |
| title_full_unstemmed | Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea |
| title_short | Comparison of Some Extraction Methods for Isolation of Catechins and Caffeine from Turkish Green Tea |
| title_sort | comparison of some extraction methods for isolation of catechins and caffeine from turkish green tea |
| topic | Turkish green tea EGCG extraction HPLC anticancer |
| url | http://ijate.net/index.php/ijsm/article/view/82 |
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