Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation

In eukaryotes, the ribosomal small subunit (40S) is composed of 18S rRNA and 33 ribosomal proteins. 18S rRNA has a special secondary structure and is an indispensable part of the translation process. Herein, a special sequence located in mammalian 18S rRNA named Poly(G)7box, which is composed of sev...

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Main Authors: Dahao Wei, Zhangyu Mai, Xinan Li, Tianli Yu, Jiangchao Li
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:RNA Biology
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Online Access:https://www.tandfonline.com/doi/10.1080/15476286.2024.2399310
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author Dahao Wei
Zhangyu Mai
Xinan Li
Tianli Yu
Jiangchao Li
author_facet Dahao Wei
Zhangyu Mai
Xinan Li
Tianli Yu
Jiangchao Li
author_sort Dahao Wei
collection DOAJ
description In eukaryotes, the ribosomal small subunit (40S) is composed of 18S rRNA and 33 ribosomal proteins. 18S rRNA has a special secondary structure and is an indispensable part of the translation process. Herein, a special sequence located in mammalian 18S rRNA named Poly(G)7box, which is composed of seven guanines, was found. Poly(G)7 can form a special and stable secondary structure by binding to the translation elongation factor subunit eEF1D and the ribosomal protein RPL32. Poly(G)7box was transfected into cells, and the translation efficiency of cells was inhibited. We believe that Poly(G)7box is an important translation-related functional element located on mammalian 18S rRNA, meanwhile the Poly(G)7 located on mRNA 5’ and 3’ box does not affect mRNA translation.
format Article
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institution DOAJ
issn 1547-6286
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language English
publishDate 2024-12-01
publisher Taylor & Francis Group
record_format Article
series RNA Biology
spelling doaj-art-1806f98e5d33452fa060b5be4993cb1a2025-08-20T02:50:04ZengTaylor & Francis GroupRNA Biology1547-62861555-85842024-12-0121189090010.1080/15476286.2024.2399310Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translationDahao Wei0Zhangyu Mai1Xinan Li2Tianli Yu3Jiangchao Li4School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, ChinaLaboratory of Oncology and Immunology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, ChinaLaboratory of Oncology and Immunology, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, ChinaLaboratory of Oncology and Immunology, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, ChinaIn eukaryotes, the ribosomal small subunit (40S) is composed of 18S rRNA and 33 ribosomal proteins. 18S rRNA has a special secondary structure and is an indispensable part of the translation process. Herein, a special sequence located in mammalian 18S rRNA named Poly(G)7box, which is composed of seven guanines, was found. Poly(G)7 can form a special and stable secondary structure by binding to the translation elongation factor subunit eEF1D and the ribosomal protein RPL32. Poly(G)7box was transfected into cells, and the translation efficiency of cells was inhibited. We believe that Poly(G)7box is an important translation-related functional element located on mammalian 18S rRNA, meanwhile the Poly(G)7 located on mRNA 5’ and 3’ box does not affect mRNA translation.https://www.tandfonline.com/doi/10.1080/15476286.2024.2399310Poly(G)7box18S rRNATranslation regulatory elementsRibosomeConserved sequence
spellingShingle Dahao Wei
Zhangyu Mai
Xinan Li
Tianli Yu
Jiangchao Li
Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation
RNA Biology
Poly(G)7box
18S rRNA
Translation regulatory elements
Ribosome
Conserved sequence
title Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation
title_full Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation
title_fullStr Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation
title_full_unstemmed Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation
title_short Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation
title_sort poly g 7 box a functional element of mammalian 18s rrna involved in translation
topic Poly(G)7box
18S rRNA
Translation regulatory elements
Ribosome
Conserved sequence
url https://www.tandfonline.com/doi/10.1080/15476286.2024.2399310
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