Generation of functional eyes from pluripotent cells.
Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this...
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Public Library of Science (PLoS)
2009-08-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1000174&type=printable |
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| author | Andrea S Viczian Eduardo C Solessio Yung Lyou Michael E Zuber |
| author_facet | Andrea S Viczian Eduardo C Solessio Yung Lyou Michael E Zuber |
| author_sort | Andrea S Viczian |
| collection | DOAJ |
| description | Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage embryos are pluripotent. Untreated, these cells formed only epidermis, when transplanted to either the flank or eye field. In contrast, misexpression of seven transcription factors induced the formation of retinal cell types. Induced retinal cells were committed to a retinal lineage as they formed eyes when transplanted to the flanks of developing embryos. When the endogenous eye field was replaced with induced retinal cells, they formed eyes that were molecularly, anatomically, and electrophysiologically similar to normal eyes. Importantly, induced eyes could guide a vision-based behavior. These results suggest the fate of pluripotent cells may be purposely altered to generate multipotent retinal progenitor cells, which differentiate into functional retinal cell classes and form a neural circuitry sufficient for vision. |
| format | Article |
| id | doaj-art-17d5aa042b354458b2bb383346ae07b3 |
| institution | OA Journals |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2009-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-17d5aa042b354458b2bb383346ae07b32025-08-20T02:00:50ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852009-08-0178e100017410.1371/journal.pbio.1000174Generation of functional eyes from pluripotent cells.Andrea S ViczianEduardo C SolessioYung LyouMichael E ZuberPluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage embryos are pluripotent. Untreated, these cells formed only epidermis, when transplanted to either the flank or eye field. In contrast, misexpression of seven transcription factors induced the formation of retinal cell types. Induced retinal cells were committed to a retinal lineage as they formed eyes when transplanted to the flanks of developing embryos. When the endogenous eye field was replaced with induced retinal cells, they formed eyes that were molecularly, anatomically, and electrophysiologically similar to normal eyes. Importantly, induced eyes could guide a vision-based behavior. These results suggest the fate of pluripotent cells may be purposely altered to generate multipotent retinal progenitor cells, which differentiate into functional retinal cell classes and form a neural circuitry sufficient for vision.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1000174&type=printable |
| spellingShingle | Andrea S Viczian Eduardo C Solessio Yung Lyou Michael E Zuber Generation of functional eyes from pluripotent cells. PLoS Biology |
| title | Generation of functional eyes from pluripotent cells. |
| title_full | Generation of functional eyes from pluripotent cells. |
| title_fullStr | Generation of functional eyes from pluripotent cells. |
| title_full_unstemmed | Generation of functional eyes from pluripotent cells. |
| title_short | Generation of functional eyes from pluripotent cells. |
| title_sort | generation of functional eyes from pluripotent cells |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1000174&type=printable |
| work_keys_str_mv | AT andreasviczian generationoffunctionaleyesfrompluripotentcells AT eduardocsolessio generationoffunctionaleyesfrompluripotentcells AT yunglyou generationoffunctionaleyesfrompluripotentcells AT michaelezuber generationoffunctionaleyesfrompluripotentcells |