The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms
Abstract Purpose To investigate the therapeutic potential of Acrorus tatarinowii in oligoasthenozoospermia and its related mechanism through modulation of the BCL2/Bax/Caspase3 signaling pathway. Methods Initially, using the TCMSP and Disgenet databases, active ingredients of Acrorus tatarinowii wer...
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BMC
2025-01-01
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| Series: | Basic and Clinical Andrology |
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| Online Access: | https://doi.org/10.1186/s12610-024-00247-w |
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| author | Zonglin Lu Haiyang Zhao Hui Wang Xin Wang Zixue Sun |
| author_facet | Zonglin Lu Haiyang Zhao Hui Wang Xin Wang Zixue Sun |
| author_sort | Zonglin Lu |
| collection | DOAJ |
| description | Abstract Purpose To investigate the therapeutic potential of Acrorus tatarinowii in oligoasthenozoospermia and its related mechanism through modulation of the BCL2/Bax/Caspase3 signaling pathway. Methods Initially, using the TCMSP and Disgenet databases, active ingredients of Acrorus tatarinowii were identified and their target genes associated with sperm-related diseases were elucidated.Subsequently, an oligoasthenozoospermia mouse model was induced and treated with Acrorus tatarinowii. Serum hormone levels were assessed by ELISA, testicular histopathology by HE staining, and target gene expression by qPCR and Western blotting. Results Acrorus tatarinowii treatment significantly upregulated BCL2 expression in the testes of oligoasthenozoospermic rat. This was accompanied by improved histopathological features in testicular tissues, reduced LH and FSH levels in serum. Conclusion Acrorus tatarinowii exerts therapeutic effects in oligoasthenozoospermia by regulating the BCL2/Bax/Caspase3 pathway, maybe by inhibiting apoptosis, and promoting germ cell proliferation. These findings highlight its potential as a natural remedy for male infertility associated with sperm function disorders. |
| format | Article |
| id | doaj-art-17cb4379493e49ec892afef34aeb31e9 |
| institution | Kabale University |
| issn | 2051-4190 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Basic and Clinical Andrology |
| spelling | doaj-art-17cb4379493e49ec892afef34aeb31e92025-01-26T12:19:53ZengBMCBasic and Clinical Andrology2051-41902025-01-0135111110.1186/s12610-024-00247-wThe protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanismsZonglin Lu0Haiyang Zhao1Hui Wang2Xin Wang3Zixue Sun4The Second Clinical Medical College, Henan University of Traditional Chinese MedicineEngineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine, Ministry of Education, Shanghai University of Traditional Chinese MedicineDepartment of Histology and Embryology, National Key Laboratory of Reproductive Medicine and Offspring Health, School of Basic Medical SciencesDepartment of Histology and Embryology, National Key Laboratory of Reproductive Medicine and Offspring Health, School of Basic Medical SciencesDepartment of Reproductive Medicine, Henan Provincial Hospital of Traditional Chinese MedicineAbstract Purpose To investigate the therapeutic potential of Acrorus tatarinowii in oligoasthenozoospermia and its related mechanism through modulation of the BCL2/Bax/Caspase3 signaling pathway. Methods Initially, using the TCMSP and Disgenet databases, active ingredients of Acrorus tatarinowii were identified and their target genes associated with sperm-related diseases were elucidated.Subsequently, an oligoasthenozoospermia mouse model was induced and treated with Acrorus tatarinowii. Serum hormone levels were assessed by ELISA, testicular histopathology by HE staining, and target gene expression by qPCR and Western blotting. Results Acrorus tatarinowii treatment significantly upregulated BCL2 expression in the testes of oligoasthenozoospermic rat. This was accompanied by improved histopathological features in testicular tissues, reduced LH and FSH levels in serum. Conclusion Acrorus tatarinowii exerts therapeutic effects in oligoasthenozoospermia by regulating the BCL2/Bax/Caspase3 pathway, maybe by inhibiting apoptosis, and promoting germ cell proliferation. These findings highlight its potential as a natural remedy for male infertility associated with sperm function disorders.https://doi.org/10.1186/s12610-024-00247-wAcrorus tatarinowiiSperm functionBCL2/Bax/Caspase3Apoptosis |
| spellingShingle | Zonglin Lu Haiyang Zhao Hui Wang Xin Wang Zixue Sun The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms Basic and Clinical Andrology Acrorus tatarinowii Sperm function BCL2/Bax/Caspase3 Apoptosis |
| title | The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms |
| title_full | The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms |
| title_fullStr | The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms |
| title_full_unstemmed | The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms |
| title_short | The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms |
| title_sort | protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms |
| topic | Acrorus tatarinowii Sperm function BCL2/Bax/Caspase3 Apoptosis |
| url | https://doi.org/10.1186/s12610-024-00247-w |
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