The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress
In this study, we first thoroughly assayed the response of the key enzymes of energy metabolism and the antioxidant system in <i>Yarrowia lipolytica</i> yeast at extreme pH. The activity of the tricarboxylic acid cycle enzymes, namely NAD-dependent isocitrate dehydrogenase, aconitate hyd...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/10/11/747 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850267027483131904 |
|---|---|
| author | Tatyana I. Rakhmanova Natalia N. Gessler Elena P. Isakova Olga I. Klein Yulia I. Deryabina Tatyana N. Popova |
| author_facet | Tatyana I. Rakhmanova Natalia N. Gessler Elena P. Isakova Olga I. Klein Yulia I. Deryabina Tatyana N. Popova |
| author_sort | Tatyana I. Rakhmanova |
| collection | DOAJ |
| description | In this study, we first thoroughly assayed the response of the key enzymes of energy metabolism and the antioxidant system in <i>Yarrowia lipolytica</i> yeast at extreme pH. The activity of the tricarboxylic acid cycle enzymes, namely NAD-dependent isocitrate dehydrogenase, aconitate hydratase, NAD-dependent malate dehydrogenase, and fumarate hydratase, NADPH-producing enzymes of glucose-6-P dehydrogenase and NADP-dependent isocitrate dehydrogenase, and the enzymes of the glutathione system was assessed. All the enzymes that were tested showed a significant induction contrary to some decrease in the aconitate hydratase activity with acidic and alkaline stress. It is probable that a change in the enzyme activity in the mitochondria matrix is involved in the regulation of the cellular metabolism of <i>Y. lipolytica</i>, which allows the species to prosper at an extreme ambient pH. It distinguishes it from any other type of ascomycete. A close relationship between the induction of the Krebs cycle enzymes and the key enzymes of the glutathione system accompanied by an increased level of reduced glutathione was shown. The assumption that the increased activity of the Krebs cycle dehydrogenases and promotion of the pentose phosphate pathway at pH stress launches a set of events determining the adaptive response of <i>Y. lipolytica</i> yeast. |
| format | Article |
| id | doaj-art-17c2bb9f3c8649a1a2b8171755e231cf |
| institution | OA Journals |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-17c2bb9f3c8649a1a2b8171755e231cf2025-08-20T01:53:57ZengMDPI AGJournal of Fungi2309-608X2024-10-01101174710.3390/jof10110747The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced StressTatyana I. Rakhmanova0Natalia N. Gessler1Elena P. Isakova2Olga I. Klein3Yulia I. Deryabina4Tatyana N. Popova5Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty, Voronezh State University, Universitetskaya pl., 1, 394000 Voronezh, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, A.N. Bach Institute of Biochemistry, Leninsky Ave. 33/2, 119071 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, A.N. Bach Institute of Biochemistry, Leninsky Ave. 33/2, 119071 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, A.N. Bach Institute of Biochemistry, Leninsky Ave. 33/2, 119071 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, A.N. Bach Institute of Biochemistry, Leninsky Ave. 33/2, 119071 Moscow, RussiaDepartment of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty, Voronezh State University, Universitetskaya pl., 1, 394000 Voronezh, RussiaIn this study, we first thoroughly assayed the response of the key enzymes of energy metabolism and the antioxidant system in <i>Yarrowia lipolytica</i> yeast at extreme pH. The activity of the tricarboxylic acid cycle enzymes, namely NAD-dependent isocitrate dehydrogenase, aconitate hydratase, NAD-dependent malate dehydrogenase, and fumarate hydratase, NADPH-producing enzymes of glucose-6-P dehydrogenase and NADP-dependent isocitrate dehydrogenase, and the enzymes of the glutathione system was assessed. All the enzymes that were tested showed a significant induction contrary to some decrease in the aconitate hydratase activity with acidic and alkaline stress. It is probable that a change in the enzyme activity in the mitochondria matrix is involved in the regulation of the cellular metabolism of <i>Y. lipolytica</i>, which allows the species to prosper at an extreme ambient pH. It distinguishes it from any other type of ascomycete. A close relationship between the induction of the Krebs cycle enzymes and the key enzymes of the glutathione system accompanied by an increased level of reduced glutathione was shown. The assumption that the increased activity of the Krebs cycle dehydrogenases and promotion of the pentose phosphate pathway at pH stress launches a set of events determining the adaptive response of <i>Y. lipolytica</i> yeast.https://www.mdpi.com/2309-608X/10/11/747<i>Yarrowia lipolytica</i>environmental stressextreme pHKrebs cycle enzymesaconitate hydratasestress defense system |
| spellingShingle | Tatyana I. Rakhmanova Natalia N. Gessler Elena P. Isakova Olga I. Klein Yulia I. Deryabina Tatyana N. Popova The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress Journal of Fungi <i>Yarrowia lipolytica</i> environmental stress extreme pH Krebs cycle enzymes aconitate hydratase stress defense system |
| title | The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress |
| title_full | The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress |
| title_fullStr | The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress |
| title_full_unstemmed | The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress |
| title_short | The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress |
| title_sort | key enzymes of carbon metabolism and the glutathione antioxidant system protect i yarrowia lipolytica i yeast against ph induced stress |
| topic | <i>Yarrowia lipolytica</i> environmental stress extreme pH Krebs cycle enzymes aconitate hydratase stress defense system |
| url | https://www.mdpi.com/2309-608X/10/11/747 |
| work_keys_str_mv | AT tatyanairakhmanova thekeyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT nataliangessler thekeyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT elenapisakova thekeyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT olgaiklein thekeyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT yuliaideryabina thekeyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT tatyananpopova thekeyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT tatyanairakhmanova keyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT nataliangessler keyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT elenapisakova keyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT olgaiklein keyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT yuliaideryabina keyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress AT tatyananpopova keyenzymesofcarbonmetabolismandtheglutathioneantioxidantsystemprotectiyarrowialipolyticaiyeastagainstphinducedstress |