Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus
In this study, an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures. By conducting single-factor experiments, Plackett-Burman design, and response surface design, the impact of 12 facto...
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| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020046 |
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| Summary: | In this study, an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures. By conducting single-factor experiments, Plackett-Burman design, and response surface design, the impact of 12 factors on co-culture intracellular polysaccharide (CC-IPS) extraction content, including extraction material-liquid ratios, extraction time, extraction temperature, and extraction solution pH were investigated. The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+ radical scavenging rates. Results showed that, the optimal extraction process parameters for the first, second, and third extractions, respectively, included material-to-liquid ratios of 1:17, 1:40, and 1:30 (g/mL), extraction times of 31, 30, and 30 min, extraction temperatures of 44, 40, and 50 ℃, and extraction solution pH levels of 3, 8, and 10. Under these optimal conditions, the CC-IPS extraction content was 86.4921±1.1924 mg/g, closed to the predicted value of 85.3981 mg/g. Additionally, the IC50 values of CC-IPS in the DPPH and ABTS+ free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL, respectively. This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures. |
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| ISSN: | 1002-0306 |