Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes
The Myrtle plant (Myrtus communis L.) is a plant species of the Myrtaceae family and a member of the maquis community, which naturally spreads in Mediterranean regions. Being resistant to arid conditions, the ecological distribution areas of the myrtle plant have been allowed to expand. The myrtle p...
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Hasan Eleroğlu
2022-08-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4892 |
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| author | Emine Açar Yıldız Aka Kaçar |
| author_facet | Emine Açar Yıldız Aka Kaçar |
| author_sort | Emine Açar |
| collection | DOAJ |
| description | The Myrtle plant (Myrtus communis L.) is a plant species of the Myrtaceae family and a member of the maquis community, which naturally spreads in Mediterranean regions. Being resistant to arid conditions, the ecological distribution areas of the myrtle plant have been allowed to expand. The myrtle plant has been used of medicinal and aromatic plants, having interesting and beautiful flowers, and rich nutrient content of the fruit in terms of valuable phytochemicals, in particular, the nutritional content of its fruits and valuable metabolites have allowed the myrtle plant to be among the healthier foods. Antioxidant activity, which neutralizes reactive oxygen species (ROS), which causes many medical problems, is one of the most important features of the myrtle plant. Investigation of the biosynthesis of anthocyanin, which leads to antioxidant activity, and determination of the biosynthesis in different tissues and genotypes is important, especially in the development of production activities. Furthermore, this study aimed to investigate the anthocyanin biosynthesis in different genotypes with white and black fruits and various tissues of genotypes. For this purpose, the expression levels of CHS, CHI, F3H and PAL genes, which are involved in the anthocyanin biosynthesis pathway, were determined by qRT-PCR. In the study, it was determined that there was an increase in the level of genes related to the biosynthesis of anthocyanin in the leaf and fruit tissues of the genotype with white fruits. It was determined that the expression level of genes related to the biosynthesis of anthocyanin was observed to be higher in the leaf and fruit tissues of the genotype with black fruits, and the highest gene expression level was found in black fruits. It was observed that anthocyanin biosynthesis was synthesized in different tissues of the plant, and anthocyanin biosynthesis was higher in fruits compared to leaves. |
| format | Article |
| id | doaj-art-17a77675aed84b1dad3a557b6ebd4b76 |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-17a77675aed84b1dad3a557b6ebd4b762025-08-20T03:54:20ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-08-011081415141910.24925/turjaf.v10i8.1415-1419.48922553Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) GenotypesEmine Açar0Yıldız Aka Kaçar1Department of Biotechnology, Institute of Natural and Applied Science, Çukurova University, 01330 AdanaDepartment of Horticulture, Faculty of Agriculture, Cukurova University, 01330 AdanaThe Myrtle plant (Myrtus communis L.) is a plant species of the Myrtaceae family and a member of the maquis community, which naturally spreads in Mediterranean regions. Being resistant to arid conditions, the ecological distribution areas of the myrtle plant have been allowed to expand. The myrtle plant has been used of medicinal and aromatic plants, having interesting and beautiful flowers, and rich nutrient content of the fruit in terms of valuable phytochemicals, in particular, the nutritional content of its fruits and valuable metabolites have allowed the myrtle plant to be among the healthier foods. Antioxidant activity, which neutralizes reactive oxygen species (ROS), which causes many medical problems, is one of the most important features of the myrtle plant. Investigation of the biosynthesis of anthocyanin, which leads to antioxidant activity, and determination of the biosynthesis in different tissues and genotypes is important, especially in the development of production activities. Furthermore, this study aimed to investigate the anthocyanin biosynthesis in different genotypes with white and black fruits and various tissues of genotypes. For this purpose, the expression levels of CHS, CHI, F3H and PAL genes, which are involved in the anthocyanin biosynthesis pathway, were determined by qRT-PCR. In the study, it was determined that there was an increase in the level of genes related to the biosynthesis of anthocyanin in the leaf and fruit tissues of the genotype with white fruits. It was determined that the expression level of genes related to the biosynthesis of anthocyanin was observed to be higher in the leaf and fruit tissues of the genotype with black fruits, and the highest gene expression level was found in black fruits. It was observed that anthocyanin biosynthesis was synthesized in different tissues of the plant, and anthocyanin biosynthesis was higher in fruits compared to leaves.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4892anthocyanin biosynthesis pathwayantioxidantgene expressionmyrtus communis lpal gene |
| spellingShingle | Emine Açar Yıldız Aka Kaçar Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes Turkish Journal of Agriculture: Food Science and Technology anthocyanin biosynthesis pathway antioxidant gene expression myrtus communis l pal gene |
| title | Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes |
| title_full | Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes |
| title_fullStr | Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes |
| title_full_unstemmed | Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes |
| title_short | Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes |
| title_sort | determination of expression level of genes associated with anthocyanin biosynthesis in some myrtle myrtus communis l genotypes |
| topic | anthocyanin biosynthesis pathway antioxidant gene expression myrtus communis l pal gene |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4892 |
| work_keys_str_mv | AT emineacar determinationofexpressionlevelofgenesassociatedwithanthocyaninbiosynthesisinsomemyrtlemyrtuscommunislgenotypes AT yıldızakakacar determinationofexpressionlevelofgenesassociatedwithanthocyaninbiosynthesisinsomemyrtlemyrtuscommunislgenotypes |