Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection

Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection

Abstract The CRISPR-based detection methods have been widely applied, yet they remain limited by the non-universal nature of one-pot diagnostic approaches. Here, we report a universal one-pot fluorescent method for the detection of epidemic pathogens, delivering results within 15-20 min. This method...

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Bibliographic Details
Main Authors: Zhou-Hua Cheng, Xi-Yan Luo, Sheng-Song Yu, Di Min, Shu-Xia Zhang, Xiao-Fan Li, Jie-Jie Chen, Dong-Feng Liu, Han-Qing Yu
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-56516-3
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