Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana.
Culture of Mycobacterium ulcerans from Buruli ulcer patients has very low sensitivity. Thus confirmation of M. ulcerans infection is primarily based on PCR directed against IS2404. In this study we compare the genotypes obtained by variable number of tandem repeat analysis of DNA from IS2404-PCR pos...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0088007 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849332351730974720 |
|---|---|
| author | Heather Williamson Richard Phillips Stephen Sarfo Mark Wansbrough-Jones Pamela Small |
| author_facet | Heather Williamson Richard Phillips Stephen Sarfo Mark Wansbrough-Jones Pamela Small |
| author_sort | Heather Williamson |
| collection | DOAJ |
| description | Culture of Mycobacterium ulcerans from Buruli ulcer patients has very low sensitivity. Thus confirmation of M. ulcerans infection is primarily based on PCR directed against IS2404. In this study we compare the genotypes obtained by variable number of tandem repeat analysis of DNA from IS2404-PCR positive cultures with that obtained from IS2404 positive, culture-negative tissue. A significantly greater genetic heterogeneity was found among culture-negative samples compared with that found in cultured strains but a single genotype is over-represented in both sample sets. This study provides evidence that both the focal location of bacteria in a lesion as well as differences in the ability to culture a particular genotype may underlie the low sensitivity of culture. Though preliminary, data from this work also suggests that mycobacteria previously associated with fish disease (M. pseudoshottsii) may be pathogenic for humans. |
| format | Article |
| id | doaj-art-1794da31b08b49d6b84ce61948a9cec8 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-1794da31b08b49d6b84ce61948a9cec82025-08-20T03:46:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8800710.1371/journal.pone.0088007Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana.Heather WilliamsonRichard PhillipsStephen SarfoMark Wansbrough-JonesPamela SmallCulture of Mycobacterium ulcerans from Buruli ulcer patients has very low sensitivity. Thus confirmation of M. ulcerans infection is primarily based on PCR directed against IS2404. In this study we compare the genotypes obtained by variable number of tandem repeat analysis of DNA from IS2404-PCR positive cultures with that obtained from IS2404 positive, culture-negative tissue. A significantly greater genetic heterogeneity was found among culture-negative samples compared with that found in cultured strains but a single genotype is over-represented in both sample sets. This study provides evidence that both the focal location of bacteria in a lesion as well as differences in the ability to culture a particular genotype may underlie the low sensitivity of culture. Though preliminary, data from this work also suggests that mycobacteria previously associated with fish disease (M. pseudoshottsii) may be pathogenic for humans.https://doi.org/10.1371/journal.pone.0088007 |
| spellingShingle | Heather Williamson Richard Phillips Stephen Sarfo Mark Wansbrough-Jones Pamela Small Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana. PLoS ONE |
| title | Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana. |
| title_full | Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana. |
| title_fullStr | Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana. |
| title_full_unstemmed | Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana. |
| title_short | Genetic diversity of PCR-positive, culture-negative and culture-positive Mycobacterium ulcerans isolated from Buruli ulcer patients in Ghana. |
| title_sort | genetic diversity of pcr positive culture negative and culture positive mycobacterium ulcerans isolated from buruli ulcer patients in ghana |
| url | https://doi.org/10.1371/journal.pone.0088007 |
| work_keys_str_mv | AT heatherwilliamson geneticdiversityofpcrpositiveculturenegativeandculturepositivemycobacteriumulceransisolatedfromburuliulcerpatientsinghana AT richardphillips geneticdiversityofpcrpositiveculturenegativeandculturepositivemycobacteriumulceransisolatedfromburuliulcerpatientsinghana AT stephensarfo geneticdiversityofpcrpositiveculturenegativeandculturepositivemycobacteriumulceransisolatedfromburuliulcerpatientsinghana AT markwansbroughjones geneticdiversityofpcrpositiveculturenegativeandculturepositivemycobacteriumulceransisolatedfromburuliulcerpatientsinghana AT pamelasmall geneticdiversityofpcrpositiveculturenegativeandculturepositivemycobacteriumulceransisolatedfromburuliulcerpatientsinghana |