Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria
Curcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/321943 |
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| author | Omer Abdalla Ahmed Hamdi Syarifah Nur Syed Abdul Rahman Khalijah Awang Norhanom Abdul Wahab Chung Yeng Looi Noel Francis Thomas Sri Nurestri Abd Malek |
| author_facet | Omer Abdalla Ahmed Hamdi Syarifah Nur Syed Abdul Rahman Khalijah Awang Norhanom Abdul Wahab Chung Yeng Looi Noel Francis Thomas Sri Nurestri Abd Malek |
| author_sort | Omer Abdalla Ahmed Hamdi |
| collection | DOAJ |
| description | Curcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human cancer cell lines (Ca Ski and MCF-7) and a noncancer cell line (HUVEC) using MTT assay. Investigation on the chemical components in the hexane and dichloromethane fractions gave 19 compounds, namely, labda-8(17),12 diene-15,16 dial (1), dehydrocurdione (2), curcumenone (3), comosone II (4), curcumenol (5), procurcumenol (6), germacrone (7), zerumbone epoxide (8), zederone (9), 9-isopropylidene-2,6-dimethyl-11-oxatricyclo[6.2.1.01,5]undec-6-en-8-ol (10), furanodiene (11), germacrone-4,5-epoxide (12), calcaratarin A (13), isoprocurcumenol (14), germacrone-1,10-epoxide (15), zerumin A (16), curcumanolide A (17), curcuzedoalide (18), and gweicurculactone (19). Compounds (1–19) were evaluated for their antiproliferative effect using MTT assay against four cancer cell lines (Ca Ski, MCF-7, PC-3, and HT-29). Curcumenone (3) and curcumenol (5) displayed strong antiproliferative activity (IC50=8.3±1.0 and 9.3±0.3 μg/mL, resp.) and were found to induce apoptotic cell death on MCF-7 cells using phase contrast and Hoechst 33342/PI double-staining assay. Thus, the present study provides basis for the ethnomedical application of Curcuma zedoaria in the treatment of breast cancer. |
| format | Article |
| id | doaj-art-179423a031a146b0bd6ee0394bdeedcd |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-179423a031a146b0bd6ee0394bdeedcd2025-02-03T01:12:39ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/321943321943Cytotoxic Constituents from the Rhizomes of Curcuma zedoariaOmer Abdalla Ahmed Hamdi0Syarifah Nur Syed Abdul Rahman1Khalijah Awang2Norhanom Abdul Wahab3Chung Yeng Looi4Noel Francis Thomas5Sri Nurestri Abd Malek6Center for Natural Products and Drugs Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaCenter for Natural Products and Drugs Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, MalaysiaCenter for Natural Products and Drugs Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, MalaysiaCenter for Natural Products and Drugs Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaCenter for Natural Products and Drugs Discovery (CENAR), University of Malaya, 50603 Kuala Lumpur, MalaysiaCurcuma zedoaria also known as Temu putih is traditionally used in food preparations and treatment of various ailments including cancer. The cytotoxic activity of hexane, dichloromethane, ethyl acetate, methanol, and the methanol-soxhlet extracts of Curcuma zedoaria rhizomes was tested on two human cancer cell lines (Ca Ski and MCF-7) and a noncancer cell line (HUVEC) using MTT assay. Investigation on the chemical components in the hexane and dichloromethane fractions gave 19 compounds, namely, labda-8(17),12 diene-15,16 dial (1), dehydrocurdione (2), curcumenone (3), comosone II (4), curcumenol (5), procurcumenol (6), germacrone (7), zerumbone epoxide (8), zederone (9), 9-isopropylidene-2,6-dimethyl-11-oxatricyclo[6.2.1.01,5]undec-6-en-8-ol (10), furanodiene (11), germacrone-4,5-epoxide (12), calcaratarin A (13), isoprocurcumenol (14), germacrone-1,10-epoxide (15), zerumin A (16), curcumanolide A (17), curcuzedoalide (18), and gweicurculactone (19). Compounds (1–19) were evaluated for their antiproliferative effect using MTT assay against four cancer cell lines (Ca Ski, MCF-7, PC-3, and HT-29). Curcumenone (3) and curcumenol (5) displayed strong antiproliferative activity (IC50=8.3±1.0 and 9.3±0.3 μg/mL, resp.) and were found to induce apoptotic cell death on MCF-7 cells using phase contrast and Hoechst 33342/PI double-staining assay. Thus, the present study provides basis for the ethnomedical application of Curcuma zedoaria in the treatment of breast cancer.http://dx.doi.org/10.1155/2014/321943 |
| spellingShingle | Omer Abdalla Ahmed Hamdi Syarifah Nur Syed Abdul Rahman Khalijah Awang Norhanom Abdul Wahab Chung Yeng Looi Noel Francis Thomas Sri Nurestri Abd Malek Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria The Scientific World Journal |
| title | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria |
| title_full | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria |
| title_fullStr | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria |
| title_full_unstemmed | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria |
| title_short | Cytotoxic Constituents from the Rhizomes of Curcuma zedoaria |
| title_sort | cytotoxic constituents from the rhizomes of curcuma zedoaria |
| url | http://dx.doi.org/10.1155/2014/321943 |
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