TLC-densitometric analysis of allantoin in Symphytum officinale L. roots
A TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The metho...
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| Format: | Article |
| Language: | English |
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Sciendo
2020-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2020-0014 |
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| author | Kimel Katarzyna Zienkiewicz Magdalena Sparzak-Stefanowska Barbara Krauze-Baranowska Mirosława |
| author_facet | Kimel Katarzyna Zienkiewicz Magdalena Sparzak-Stefanowska Barbara Krauze-Baranowska Mirosława |
| author_sort | Kimel Katarzyna |
| collection | DOAJ |
| description | A TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH2 100A column, with mobile phase consisting of acetonitrile/water 80:20 (V/V) and UV detection at 190 and 210 nm. Allantoin content was determined in two herbal products and it varied from 0.94 to 2.09 %, depending on the producer, and was in agreement with literature reports. |
| format | Article |
| id | doaj-art-178fd8f000bf460ebcb7794f555004a7 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-178fd8f000bf460ebcb7794f555004a72025-02-03T06:59:07ZengSciendoActa Pharmaceutica1846-95582020-03-0170110111010.2478/acph-2020-0014acph-2020-0014TLC-densitometric analysis of allantoin in Symphytum officinale L. rootsKimel Katarzyna0Zienkiewicz Magdalena1Sparzak-Stefanowska Barbara2Krauze-Baranowska Mirosława3Medical University of Gdańsk, Department of Pharmacognosy with the Medicinal Plants Garden, Gdańsk, PolandMedical University of Gdańsk, Department of Pharmacognosy with the Medicinal Plants Garden, Gdańsk, PolandMedical University of Gdańsk, Department of Pharmacognosy with the Medicinal Plants Garden, Gdańsk, PolandMedical University of Gdańsk, Department of Pharmacognosy with the Medicinal Plants Garden, Gdańsk, PolandA TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH2 100A column, with mobile phase consisting of acetonitrile/water 80:20 (V/V) and UV detection at 190 and 210 nm. Allantoin content was determined in two herbal products and it varied from 0.94 to 2.09 %, depending on the producer, and was in agreement with literature reports.https://doi.org/10.2478/acph-2020-0014allantoinsymphytum officinaledensitometrytlchplc |
| spellingShingle | Kimel Katarzyna Zienkiewicz Magdalena Sparzak-Stefanowska Barbara Krauze-Baranowska Mirosława TLC-densitometric analysis of allantoin in Symphytum officinale L. roots Acta Pharmaceutica allantoin symphytum officinale densitometry tlc hplc |
| title | TLC-densitometric analysis of allantoin in Symphytum officinale L. roots |
| title_full | TLC-densitometric analysis of allantoin in Symphytum officinale L. roots |
| title_fullStr | TLC-densitometric analysis of allantoin in Symphytum officinale L. roots |
| title_full_unstemmed | TLC-densitometric analysis of allantoin in Symphytum officinale L. roots |
| title_short | TLC-densitometric analysis of allantoin in Symphytum officinale L. roots |
| title_sort | tlc densitometric analysis of allantoin in symphytum officinale l roots |
| topic | allantoin symphytum officinale densitometry tlc hplc |
| url | https://doi.org/10.2478/acph-2020-0014 |
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